Reactive oxygen species explicit dosimetry for the evaluations of treatment efficiency of single and fractionated ALA-mediated photodynamic therapy

Reactive oxygen species explicit dosimetry (ROSED) has been recently developed and demonstrated to be able to better correlate with treatment outcome in both preclinical type I and type II PDT. ROSED involves direct measurements of in-vivo light fluence (rate), in-vivo photosensitizer concentration and tissue oxygenation to calculate for reacted ROS concentration ([ROS]rx) generated by PDT processes. In this study, we demonstrated ROSED in ALA-mediated PDT for mice bearing radiation-induced fibrosacorma (RIF) tumor. PDT treatments were performed using single or fractionated illumination to a same total fluence of 140Jcm-2. The effects of light fractionation on the total reacted [ROS]rx and treatment outcomes were evaluated.

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