Raman spectroscopic investigation of 13CO2 labeling and leaf dark respiration of Fagus sylvatica L. (European beech)

An important issue, in times of climate change and more extreme weather events, is the investigation of forest ecosystem reactions to these events. Longer drought periods stress the vitality of trees and promote mass insect outbreaks, which strongly affect ecosystem processes and services. Cavity-enhanced Raman gas spectrometry was applied for online multi-gas analysis of the gas exchange rates of O2 and CO2 and the labeling of Fagus sylvatica L. (European beech) seedlings with 13CO2. The rapid monitoring of all these gases simultaneously allowed for the separation of photosynthetic uptake of CO2 by the beech seedlings and a constant 12CO2 efflux via respiration and thus for a correction of the measured 12CO2 concentrations in course of the labeling experiment. The effects of aphid infestation with the woolly beech aphid (Phyllaphis fagi L.) as well as the effect of a drought period on the respirational gas exchange were investigated. A slightly decreased respirational activity of drought-stressed seedlings in comparison to normally watered seedlings was found already for a low drought intensity. Cavity-enhanced Raman gas monitoring of O2, 12CO2, and 13CO2 was proven to be a powerful new tool for studying the effect of drought stress and aphid infestation on the respirational activity of European beech seedlings as an example of important forest species in Central Europe.

[1]  N. Breda,et al.  Water transfer in a mature oak stand (Quercus petraea) : Seasonal evolution and effects of a severe drought , 1993 .

[2]  Jürgen Popp,et al.  Morphology-sensitive Raman modes of the malaria pigment hemozoin. , 2009, The Analyst.

[3]  H. Pretzsch,et al.  Resistance of European tree species to drought stress in mixed versus pure forests: evidence of stress release by inter-specific facilitation. , 2013, Plant biology.

[4]  R. G. Smith.  Wax glands, wax production and the functional significance of wax use in three aphid species (Homoptera: Aphididae) , 1999 .

[5]  J. Popp,et al.  Relationship between molecular structure and Raman spectra of quinolines , 2009 .

[6]  Jürgen Popp,et al.  Fiber-enhanced Raman multigas spectroscopy: a versatile tool for environmental gas sensing and breath analysis. , 2014, Analytical chemistry.

[7]  N. McDowell,et al.  Mechanisms of plant survival and mortality during drought: why do some plants survive while others succumb to drought? , 2008, The New phytologist.

[8]  Jürgen Popp,et al.  Fast and highly sensitive fiber-enhanced Raman spectroscopic monitoring of molecular H2 and CH4 for point-of-care diagnosis of malabsorption disorders in exhaled human breath. , 2015, Analytical chemistry.

[9]  Jürgen Popp,et al.  Enhanced Raman multigas sensing - a novel tool for control and analysis of (13)CO(2) labeling experiments in environmental research. , 2014, The Analyst.

[10]  Jürgen Popp,et al.  Structural analysis of the antimalarial drug halofantrine by means of Raman spectroscopy and density functional theory calculations. , 2010, Journal of biomedical optics.

[11]  A. Lugo,et al.  Climate Change and Forest Disturbances , 2001 .

[12]  Jürgen Popp,et al.  Raman spectroscopy-an innovative and versatile tool to follow the respirational activity and carbonate biomineralization of important cave bacteria. , 2013, Analytical chemistry.

[13]  Manfred J. Lexer,et al.  Impact of bark beetle (Ips typographus L.) disturbance on timber production and carbon sequestration in different management strategies under climate change , 2008 .

[14]  Jürgen Popp,et al.  Ultrasensitive fiber enhanced UV resonance Raman sensing of drugs. , 2013, Analytical chemistry.

[15]  D. A. King,et al.  Simple diode pumping of a power-buildup cavity. , 1998, Optics letters.

[16]  H. Rennenberg,et al.  The effect of drought on C and N stable isotopes in different fractions of leaves, stems and roots of sensitive and tolerant beech ecotypes. , 2006, Plant, cell & environment.

[17]  Jürgen Popp,et al.  Investigation of gas exchange processes in peat bog ecosystems by means of innovative Raman gas spectroscopy. , 2013, Analytical chemistry.

[18]  J. Edwards.  Defence by Smear: Supercooling in the Cornicle Wax of Aphids , 1966, Nature.