MITOSIS AND INTERMEDIATE-SIZED FILAMENTS IN DEVELOPING SKELETAL MUSCLE

A new class of filaments intermediate in diameter between actin and myosin filaments has been demonstrated in skeletal muscle cells cultured from chick embryos. These filaments, which account for the majority of free filaments, average 100 A in diameter. They may run for more than 2 µ in a single section and can be distinguished in size and appearance from the thick and thin filaments assembled into myofibrils. The 100-A filaments are seen scattered throughout the sarcoplasm at all stages of development and show no obvious association with the myofibrils. The 100-A filaments are particularly conspicuous in myotubes fragmented by the mitotic inhibitors, colchicine and Colcemid. In addition, filaments similar in size and appearance to those found in myotubes are present in fibroblasts, chondrocytes, and proliferating mononucleated myoblasts. The 100-A filaments are present in cells arrested in metaphase by mitotic inhibitors. Definitive thick (about 150 A) or thin (about 60 A) myofilaments are not found in skeletal myogenic cells arrested in metaphase. Myogenic cells arrested in metaphase do not bind fluorescein-labeled antibody directed against myosin or actin. For these reasons, it is concluded that not all "thin" filaments in myogenic cells are uniquely associated with myogenesis.

[1]  A. Szent-Györgyi.  A new method for the preparation of actin. , 1951 .

[2]  A. Szent-Gyorgyi.  A new method for the preparation of actin. , 1951, The Journal of biological chemistry.

[3]  G. Godman.  The effect of colchicine on striated muscle in tissue culture. , 1955, Experimental cell research.

[4]  J. Preer.  A quantitative study of a technique of double diffusion in agar. , 1956, Journal of immunology.

[5]  H. Huxley,et al.  Quantitative studies on the structure of cross-striated myofibrils. II. Investigations by biochemical techniques. , 1957, Biochimica et biophysica acta.

[6]  H. Holtzer,et al.  AN ANALYSIS OF MYOGENESIS BY THE USE OF FLUORESCENT ANTIMYOSIN , 1957, The Journal of biophysical and biochemical cytology.

[7]  H. Holtzer,et al.  The distribution of protein antigens in striated myofibrils , 1959 .

[8]  F. Stockdale,et al.  DNA synthesis and myogenesis. , 1961, Experimental cell research.

[9]  Albert Claude.  Mise en évidence par microscopie électronique d'un appareil fibrillaire dans le cytoplasme et le noyau de certaines cellules , 1961 .

[10]  D. Mazia,et al.  Mitosis and the Physiology of Cell Division , 1961 .

[11]  S. de Petris,et al.  Filamentous structures in the cytoplasm of normal mononuclear phagocytes. , 1962, Journal of ultrastructure research.

[12]  B. Strehler,et al.  PLOIDY OF MYOTUBE NUCLEI DEVELOPING IN VITRO AS DETERMINED WITH A RECORDING DOUBLE BEAM MICRO-SPECTROPHOTOMETER. , 1963, Experimental cell research.

[13]  R. Bassleer.  APPLICATION DE TECHNIQUES CYTOLOGIQUES MODERNES À L'ÉTUDE DU PROBLEME DES AMITOSES DANS LES BOURGEONS MUSCULAIRES , 1963 .

[14]  N. Gonatas,et al.  HISTOCHEMICAL AND ULTRASTRUCTURAL STUDIES ON HELA CELL CULTURES EXPOSED TO SPINDLE INHIBITORS WITH SPECIAL REFERENCE TO THE INTERPHASE CELL , 1964, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[15]  K. Okazaki,et al.  5-Bromodeoxyuridine: Effect on Myogenesis in vitro , 1964, Science.

[16]  H. Price,et al.  ULTRASTRUCTURAL ALTERATIONS IN SKELETAL MUSCLE FIBERS INJURED BY COLD. I. THE ACUTE DEGENERATIVE CHANGES. , 1964, Laboratory investigation; a journal of technical methods and pathology.

[17]  H. Price,et al.  ULTRASTRUCTURAL ALTERATIONS IN SKELETAL MUSCLE FIBERS INJURED BY COLD. II. CELLS ON THE SARCOLEMMAL TUBE: OBSERVATIONS ON "DISCONTINUOUS" REGENERATION AND MYOFIBRIL FORMATION. , 1964, Laboratory investigation; a journal of technical methods and pathology.

[18]  K. Wohlfarth-Bottermann.  Differentiations of the Ground Cytoplasm and Their Significance for the Generation of the Motive Force of Ameboid Movement1 , 1964 .

[19]  Y. Tanaka,et al.  FIBRILLAR STRUCTURES IN THE CELLS OF BLOODFORMING ORGANS. , 1964, Journal of the National Cancer Institute.

[20]  L. Wolpert,et al.  Studies on the Isolated Membrane and Cytoplasm of Amoeba proteus in Relation to Ameboid Movement , 1964 .

[21]  E. R. Allen,et al.  ULTRASTRUCTURE OF DEVELOPING MUSCLE CELLS IN THE CHICK EMBRYO. , 1965, The American journal of anatomy.

[22]  K. Okazaki,et al.  AN ANALYSIS OF MYOGENESIS IN VITRO USING FLUORESCEIN-LABELED ANTIMYOSIN , 1965, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.

[23]  P. Biberfeld,et al.  Increased occurrence of cytoplasmic filaments in in vitro propagated rat liver epithelial cells. , 1965, Experimental cell research.

[24]  石川 春律.  The fine structure of myo-tendon junction in some mammalian skeletal muscles , 1965 .

[25]  Przybylski Rj,et al.  Ultrastructural aspects of myogenesis in the chick. , 1966 .

[26]  K. Thimann,et al.  Intracellular fibers in oat coleoptile cells and their possible significance in cytoplasmic streaming. , 1966, Proceedings of the National Academy of Sciences of the United States of America.

[27]  R. Przybylski,et al.  Ultrastructural aspects of myogenesis in the chick. , 1966, Laboratory investigation; a journal of technical methods and pathology.

[28]  K. Okazaki,et al.  Myogenesis: fusion, myosin synthesis, and the mitotic cycle. , 1966, Proceedings of the National Academy of Sciences of the United States of America.

[29]  A. C. Taylor.  MICROTUBULES IN THE MICROSPIKES AND CORTICAL CYTOPLASM OF ISOLATED CELLS , 1966, The Journal of cell biology.

[30]  T. Obinata,et al.  The identification of randomly formed thin filaments in differentiating muscle cells of the chick embryo. , 1966, Developmental biology.

[31]  L. Rebhun,et al.  Cytoplasmic microfilaments in streaming Nitella cells. , 1966, Journal of ultrastructure research.

[32]  E. Robbins,et al.  CHAPTER 18 – Some Macromolecular Characteristics of Synchronized HeLa Cells , 1966 .

[33]  D. Fischman.  AN ELECTRON MICROSCOPE STUDY OF MYOFIBRIL FORMATION IN EMBRYONIC CHICK SKELETAL MUSCLE , 1967, The Journal of cell biology.

[34]  Shinya Inoué,et al.  Cell Motility by Labile Association of Molecules , 1967, The Journal of general physiology.

[35]  H. Ishikawa,et al.  DIFFERENTIATION OF THE SARCOPLASMIC RETICULUM AND T SYSTEM IN DEVELOPING CHICK SKELETAL MUSCLE IN VITRO , 1967, The Journal of cell biology.

[36]  T. Hsu,et al.  The effects of colcemid inhibition and reversal on the fine structure of the mitotic apparatus of Chinese hamster cells in vitro. , 1967, Journal of ultrastructure research.

[37]  M. A. Gorycki,et al.  An electron microscopic study of regeneration and satellite cells in human muscle , 1967, Neurology.

[38]  H. Ishikawa.  FORMATION OF ELABORATE NETWORKS OF T-SYSTEM TUBULES IN CULTURED SKELETAL MUSCLE WITH SPECIAL REFERENCE TO THE T-SYSTEM FORMATION , 1968, The Journal of cell biology.

[39]  F. Manasek,et al.  MITOSIS IN DEVELOPING CARDIAC MUSCLE , 1968, The Journal of cell biology.

[40]  J. Abbott,et al.  Oscillations of the Chondrogenic Phenotype in vitro , 1968 .

[41]  H. Holtzer,et al.  THE EFFECT OF MITOTIC INHIBITORS ON MYOGENESIS IN VITRO , 1968, The Journal of cell biology.