Wetting effects on in vitro bioactivity and in vitro biocompatibility of laser micro-textured Ca-P coating

Calcium phosphate (Ca-P) coating on the Ti-6Al-4V alloy enhances osteoblast adhesion and tissue formation at the bone implant interface. In light of this, in the current work a laser-based coating technique was used to synthesize two different micro-textured (100 microm and 200 microm spaced line patterns) Ca-P coatings on the Ti-6Al-4V alloy and its effect on wettability and osteoblast cell adhesion were systematically studied. X-ray diffraction (XRD) analysis of the coated samples indicated the presence of precursor material, Ca10(PO4)6(OH)2 (HA) and various other additional phases such as CaTiO3, Ca3(PO4)2, TiO2 (anatase) and TiO2 (rutile) owing to the reaction between the precursor (HA) and substrate (Ti-6Al-4V) during laser processing. Confocal laser scanning microscopy-based characterization of coated samples indicated that the samples processed at 100 microm line spacing demonstrated a reduced surface roughness and smaller texture parameter value as compared to the samples processed at 200 microm spacing. The surface energy and wettability of the 100 microm spaced samples measured using a static sessile drop technique demonstrated higher surface energy and increased hydrophilicity as compared to the control (untreated Ti-6Al-4V) and the samples processed at 200 microm spacing. The tendency of coated samples for mineralization through generation of an apatite-like phase during immersion in a simulated body fluid was indicative of their in vitro bioactive nature. In light of higher surface energy and increased hydrophilicity the in vitro biocompatibility of the samples with 100 microm line spacing was demonstrated through increased cell proliferation and cell adhesion of mouse MC3T3-E1 osteoblast-like cells.

[1]  D. Grant,et al.  The effect of surface chemistry and nanotopography of titanium nitride (TiN) films on 3T3-L1 fibroblasts. , 2003, Journal of biomedical materials research. Part A.

[2]  P. Janmey,et al.  Tissue Cells Feel and Respond to the Stiffness of Their Substrate , 2005, Science.

[3]  M. Shirkhanzadeh.  Bioactive calcium phosphate coatings prepared by electrodeposition , 1991 .

[4]  Pedro L Granja,et al.  The correlation between the adsorption of adhesive proteins and cell behaviour on hydroxyl-methyl mixed self-assembled monolayers. , 2009, Biomaterials.

[5]  N. Dahotre,et al.  Laser surface treatment for porous and textured Ca–P bio-ceramic coating on Ti–6Al–4V , 2007, Biomedical materials.

[6]  Lisa A Flanagan,et al.  Neurite branching on deformable substrates , 2002, Neuroreport.

[7]  Boris N. Chichkov,et al.  The hydrophobic properties of femtosecond laser fabricated spike structures and their effects on cell proliferation , 2009 .

[8]  Johanna Ivaska,et al.  Novel functions of vimentin in cell adhesion, migration, and signaling. , 2007, Experimental cell research.

[9]  Melitta Schachner,et al.  Recognition molecules and neural repair , 2007, Journal of neurochemistry.

[10]  P. Roach,et al.  Modern biomaterials: a review—bulk properties and implications of surface modifications , 2007, Journal of materials science. Materials in medicine.

[11]  B. König,et al.  In vitro and in vivo biocompatibility testing of Ti-6Al-7Nb alloy with and without plasma-sprayed hydroxyapatite coating. , 2001, Journal of biomedical materials research.

[12]  N. Dahotre,et al.  Temporally evolved recoil pressure driven melt infiltration during laser surface modifications of porous alumina ceramic , 2007 .

[13]  S. Howdle,et al.  Osteoblast growth on titanium foils coated with hydroxyapatite by pulsed laser ablation. , 2001, Biomaterials.

[14]  Joyce Y Wong,et al.  Evaluation of polydimethylsiloxane scaffolds with physiologically-relevant elastic moduli: interplay of substrate mechanics and surface chemistry effects on vascular smooth muscle cell response. , 2005, Biomaterials.

[15]  Benjamin M. Wu,et al.  Cell interaction with three-dimensional sharp-tip nanotopography. , 2007, Biomaterials.

[16]  S. Olenych,et al.  Vascular smooth muscle cells on polyelectrolyte multilayers: hydrophobicity-directed adhesion and growth. , 2005, Biomacromolecules.

[17]  M. Dembo,et al.  Cell movement is guided by the rigidity of the substrate. , 2000, Biophysical journal.

[18]  J. Itskovitz‐Eldor,et al.  Differentiation of human embryonic stem cells on three-dimensional polymer scaffolds , 2003, Proceedings of the National Academy of Sciences of the United States of America.

[19]  A Ranella,et al.  Biomimetic micro∕nanostructured functional surfaces for microfluidic and tissue engineering applications. , 2011, Biomicrofluidics.

[20]  K. Jandt,et al.  Tuning Cell Adhesion on PTFE Surfaces by Laser Induced Microstructures , 2007 .

[21]  Wei Liu,et al.  Collagen Tissue Engineering: Development of Novel Biomaterials and Applications , 2008, Pediatric Research.

[22]  J. Fischer,et al.  Elastic Fully Three‐dimensional Microstructure Scaffolds for Cell Force Measurements , 2010, Advanced materials.

[23]  S. Sen,et al.  Matrix Elasticity Directs Stem Cell Lineage Specification , 2006, Cell.

[24]  David Boettiger,et al.  Mechanically Activated Integrin Switch Controls α5β1 Function , 2009, Science.

[25]  C. V. Oss,et al.  Reevaluation of the surface tension components and parameters of polyacetylene from contact angles of liquids , 1990 .

[26]  Kheya Sengupta,et al.  Fibroblast adaptation and stiffness matching to soft elastic substrates. , 2007, Biophysical journal.

[27]  Xian-Jin Yang,et al.  Improving the biocompatibility of NiTi alloy by chemical treatments: An in vitro evaluation in 3T3 human fibroblast cell , 2008 .

[28]  Joe Tien,et al.  Repositioning of cells by mechanotaxis on surfaces with micropatterned Young's modulus. , 2003, Journal of biomedical materials research. Part A.

[29]  C. Murphy,et al.  Responses of human keratocytes to micro- and nanostructured substrates. , 2004, Journal of biomedical materials research. Part A.

[30]  C. Murphy,et al.  Epithelial contact guidance on well-defined micro- and nanostructured substrates , 2003, Journal of Cell Science.

[31]  C. Fotakis,et al.  Tailoring the wetting response of silicon surfaces via fs laser structuring , 2008 .

[32]  Xiaolong Zhu,et al.  Effect of hydrothermally treated anodic oxide films on osteoblast attachment and proliferation. , 2003, Biomaterials.

[33]  Matthew John Dalby,et al.  Changes in fibroblast morphology in response to nano-columns produced by colloidal lithography. , 2004, Biomaterials.

[34]  Xiaolong Zhu,et al.  Cellular Reactions of Osteoblasts to Micron- and Submicron-Scale Porous Structures of Titanium Surfaces , 2004, Cells Tissues Organs.

[35]  K. Kendall,et al.  Surface energy and the contact of elastic solids , 1971, Proceedings of the Royal Society of London. A. Mathematical and Physical Sciences.

[36]  Timothy D. Veenstra,et al.  Mechanically Activated Integrin Switch Controls a 5 b 1 Function , 2009 .

[37]  B. Meenan,et al.  Surface characterisation of the evolving nature of radio frequency (RF) magnetron sputter deposited calcium phosphate thin films after exposure to physiological solution , 2006 .

[38]  J. Davies,et al.  Platelet interactions with calcium-phosphate-coated surfaces. , 2005, Biomaterials.

[39]  Ion N. Mihailescu,et al.  Pulsed laser deposition of hydroxyapatite thin films on Ti-5Al-2.5Fe substrates with and without buffer layers , 2000 .

[40]  M. Hindié,et al.  Interactions of B16F10 melanoma cells aggregated on a cellulose substrate , 2006, Journal of cellular biochemistry.

[41]  R. Sandberg,et al.  Gene expression perturbation in vitro--a growing case for three-dimensional (3D) culture systems. , 2005, Seminars in cancer biology.

[42]  Christine E Schmidt,et al.  Nanostructured scaffolds for neural applications. , 2008, Nanomedicine.

[43]  A. Giakoumaki,et al.  Tailor-made three-dimensional hybrid scaffolds for cell cultures , 2011, Biomedical materials.

[44]  P Zioupos,et al.  Mechanical properties and the hierarchical structure of bone. , 1998, Medical engineering & physics.

[45]  S. Bhatia,et al.  Tissue Engineering at the Micro-Scale , 1999 .

[46]  Kenneth M. Yamada,et al.  Taking Cell-Matrix Adhesions to the Third Dimension , 2001, Science.

[47]  P. Martin,et al.  Incorporation of Si and SiO(x) into diamond-like carbon films: impact on surface properties and osteoblast adhesion. , 2009, Acta biomaterialia.

[48]  Olle Inganäs,et al.  The promotion of neuronal maturation on soft substrates. , 2009, Biomaterials.

[49]  Joyce Y Wong,et al.  Neurite outgrowth and branching of PC12 cells on very soft substrates sharply decreases below a threshold of substrate rigidity , 2007, Journal of neural engineering.

[50]  Paolo A Netti,et al.  A multi-functional scaffold for tissue regeneration: the need to engineer a tissue analogue. , 2007, Biomaterials.

[51]  P. Ramires,et al.  The influence of titania/hydroxyapatite composite coatings on in vitro osteoblasts behaviour. , 2001, Biomaterials.

[52]  N. Dahotre,et al.  Fabrication and evaluation of a pulse laser-induced Ca–P coating on a Ti alloy for bioapplication , 2009, Biomedical materials.

[53]  L. Reichardt,et al.  Extracellular Matrix 2: Role of extracellular matrix molecules and their receptors in the nervous system , 1993, FASEB journal : official publication of the Federation of American Societies for Experimental Biology.

[54]  J. Jacobs,et al.  Evaluation of metallic and polymeric biomaterial surface energy and surface roughness characteristics for directed cell adhesion. , 2001, Tissue engineering.

[55]  Donald E Ingber,et al.  Micromechanical control of cell and tissue development: implications for tissue engineering. , 2007, Advanced drug delivery reviews.

[56]  B. Boyan,et al.  Requirement for both micron- and submicron scale structure for synergistic responses of osteoblasts to substrate surface energy and topography. , 2007, Biomaterials.

[57]  Peter Friedl,et al.  Cell migration strategies in 3‐D extracellular matrix: Differences in morphology, cell matrix interactions, and integrin function , 1998, Microscopy research and technique.

[58]  Julian H. George,et al.  Exploring and Engineering the Cell Surface Interface , 2005, Science.

[59]  Costas Fotakis,et al.  Biomimetic Artificial Surfaces Quantitatively Reproduce the Water Repellency of a Lotus Leaf , 2008 .

[60]  J. Vacanti,et al.  Microfabrication Technology for Vascularized Tissue Engineering , 2002 .

[61]  Narendra B. Dahotre,et al.  Prediction of solidification microstructures during laser dressing of alumina-based grinding wheel material , 2006 .

[62]  David J. Mooney,et al.  Growth Factors, Matrices, and Forces Combine and Control Stem Cells , 2009, Science.

[63]  A. Duncan,et al.  Laser microfabricated model surfaces for controlled cell growth. , 2002, Biosensors & bioelectronics.

[64]  D. Ingber,et al.  Cellular tensegrity : defining new rules of biological design that govern the cytoskeleton , 2022 .

[65]  Seeram Ramakrishna,et al.  Potential of nanofiber matrix as tissue-engineering scaffolds. , 2005, Tissue engineering.

[66]  John A. Pedersen,et al.  Mechanobiology in the Third Dimension , 2005, Annals of Biomedical Engineering.

[67]  Marion Ghibaudo,et al.  Rigidity-driven growth and migration of epithelial cells on microstructured anisotropic substrates , 2007, Proceedings of the National Academy of Sciences.

[68]  C. B. Ponton,et al.  Microstructural characterization of hydroxyapatite coating on titanium , 1992 .

[69]  D. Haddow,et al.  Sol-Gel Derived Calcium Phosphate Coatings for Biomedical Applications , 1998 .

[70]  Narendra B. Dahotre,et al.  Review paper: Surface Modification for Bioimplants: The Role of Laser Surface Engineering , 2005, Journal of biomaterials applications.

[71]  Y. Wang,et al.  Cell locomotion and focal adhesions are regulated by substrate flexibility. , 1997, Proceedings of the National Academy of Sciences of the United States of America.

[72]  C J Murphy,et al.  Effects of synthetic micro- and nano-structured surfaces on cell behavior. , 1999, Biomaterials.

[73]  J. Adams.  Cell-matrix contact structures , 2001, Cellular and Molecular Life Sciences CMLS.

[74]  Caterina Minelli,et al.  Substrate stiffness affects early differentiation events in embryonic stem cells. , 2009, European cells & materials.

[75]  Zhong Lin Wang,et al.  Aspect ratio dependence of the elastic properties of ZnO nanobelts. , 2007, Nano letters.

[76]  Costas Fotakis,et al.  Laser-based micro/nanoengineering for biological applications , 2009 .

[77]  W. Wagner,et al.  Effects of hydroxylapatite coating crystallinity on biosolubility, cell attachment efficiency and proliferation in vitro. , 1999, Biomaterials.

[78]  David J. Mooney,et al.  Non-viral gene delivery regulated by stiffness of cell adhesion substrates , 2005, Nature materials.

[79]  J. D. Hosson,et al.  Influence of surface roughness on the wetting angle , 1995 .

[80]  C. Fotakis,et al.  Tuning cell adhesion by controlling the roughness and wettability of 3D micro/nano silicon structures. , 2010, Acta biomaterialia.

[81]  M. Chaudhury,et al.  Additive and nonadditive surface tension components and the interpretation of contact angles , 1988 .

[82]  D. Gottlieb,et al.  Optimization of fibrin scaffolds for differentiation of murine embryonic stem cells into neural lineage cells. , 2006, Biomaterials.

[83]  William P King,et al.  Myoblast alignment and differentiation on cell culture substrates with microscale topography and model chemistries. , 2007, Biomaterials.

[84]  M. Marinkovich,et al.  Bridging structure with function: structural, regulatory, and developmental role of laminins. , 2008, The international journal of biochemistry & cell biology.

[85]  Hung-Ta Wang,et al.  The control of cell adhesion and viability by zinc oxide nanorods. , 2008, Biomaterials.

[86]  M. Textor,et al.  Surface engineering approaches to micropattern surfaces for cell-based assays. , 2006, Biomaterials.

[87]  Kam W Leong,et al.  Nanopattern-induced changes in morphology and motility of smooth muscle cells. , 2005, Biomaterials.

[88]  A. Klukowska,et al.  Novel transparent hybrid polymer working stamp for UV-imprinting , 2009 .

[89]  Daniel Choquet,et al.  Extracellular Matrix Rigidity Causes Strengthening of Integrin–Cytoskeleton Linkages , 1997, Cell.

[90]  L. Backman.  Shape changes of the human red cell studied by aqueous two‐phase partition , 1990, FEBS letters.

[91]  Replica molding of picosecond laser fabricated Si microstructures , 2007 .

[92]  S. Hansson,et al.  The relation between surface roughness and interfacial shear strength for bone-anchored implants. A mathematical model. , 1999, Journal of biomechanics.

[93]  D. Hutmacher,et al.  Scaffolds in tissue engineering bone and cartilage. , 2000, Biomaterials.

[94]  B. Tang,et al.  Response of power-law-viscoelastic and time-dependent materials to rate jumps , 2009 .

[95]  F. Bäckhed,et al.  Nanoscale features influence epithelial cell morphology and cytokine production. , 2003, Biomaterials.

[96]  N. Dahotre,et al.  Wettability and kinetics of hydroxyapatite precipitation on a laser-textured Ca-P bioceramic coating. , 2009, Acta biomaterialia.