Anaerobic co-digestion of desugared molasses with cow manure; focusing on sodium and potassium inhibition.

Desugared molasses (DM), a syrup residue from beet-molasses, was investigated for biogas production in both batch and in continuously-stirred tank reactor (CSTR) experiments. DM contained 2-3 times higher concentration of ions than normal molasses, which could inhibit the biogas process. The effect of sodium and potassium concentration on biogas production from manure was also investigated. Fifty percent inhibition occurred at sodium and potassium concentration of 11 and 28 g/L, respectively. The reactor experiments were carried out to investigate the biogas production from DM under different dilutions with water and co-digestion with manure. Stable operation at maximum methane yield of 300 mL-CH4/gVS-added was obtained at a mixture of 5% DM in cow manure. The biogas process was inhibited at DM concentrations higher than 15%. Manure was a good base substrate for co-digestion, and a stable anaerobic digestion could be achieved by co-digesting DM with manure at the concentration below 15% DM.

[1]  W. Payne EFFECTS OF SODIUM AND POTASSIUM IONS ON GROWTH AND SUBSTRATE PENETRATION OF A MARINE PSEUDOMONAD , 1960, Journal of bacteriology.

[2]  B. Ahring,et al.  Anaerobic digestion of swine manure: Inhibition by ammonia , 1998 .

[3]  A. Guwy,et al.  Defining the biomethane potential (BMP) of solid organic wastes and energy crops: a proposed protocol for batch assays. , 2009, Water science and technology : a journal of the International Association on Water Pollution Research.

[4]  I Angelidaki,et al.  Compact automated displacement gas metering system for measurement of low gas rates from laboratory fermentors. , 1992, Biotechnology and bioengineering.

[5]  A. E. Greenberg,et al.  Standard methods for the examination of water and wastewater : supplement to the sixteenth edition , 1988 .

[6]  I. Angelidaki,et al.  Optimization of biogas production from olive-oil mill wastewater, by codigesting with diluted poultry-manure , 2007 .

[7]  P. He,et al.  Effect of Alkali Metal Cation on the Anaerobic Hydrolysis and Acidogenesis of Vegetable Waste , 2006, Environmental technology.

[8]  Dae Sung Lee,et al.  Comprehensive study on a two-stage anaerobic digestion process for the sequential production of hydrogen and methane from cost-effective molasses , 2010 .

[9]  I. J. Kugelman,et al.  CATION TOXICITY AND STIMULATION IN ANAEROBIC WASTE TREATMENT , 1965 .

[10]  E. Trably,et al.  Effect of post-digestion temperature on serial CSTR biogas reactor performance. , 2009, Water research.

[11]  J. Caton,et al.  Effects of concentrated separator by-product (desugared molasses) on intake, ruminal fermentation, digestion, and microbial efficiency in beef steers fed grass hay. , 2006, Journal of animal science.

[12]  N. Bernet,et al.  Interactions between methanogenic and nitrate reducing bacteria during the anaerobic digestion of an industrial sulfate rich wastewater , 1999 .

[13]  Trong Hoan Do,et al.  The effect of calcium on the anaerobic digestion treating swine wastewater , 2006 .

[14]  B. Ahring,et al.  Thermophilic anaerobic digestion of livestock waste: the effect of ammonia , 2004, Applied Microbiology and Biotechnology.

[15]  Thomas D. Brock,et al.  Biology of microorganisms , 1970 .

[16]  M. Balakrishnan,et al.  Wastewater treatment in molasses-based alcohol distilleries for COD and color removal: a review. , 2008, Journal of environmental management.

[17]  J. Pfaff METHOD 300.0 – DETERMINATION OF INORGANIC ANIONS BY ION CHROMATOGRAPHY , 1996 .

[18]  Irini Angelidaki Anaerobic digestion in Denmark. Past, present and future , 1997 .

[19]  Willy Verstraete,et al.  Influence of high NaCl and NH4Cl salt levels on methanogenic associations , 1984 .

[20]  M. Kornaros,et al.  Biogas production from anaerobic co-digestion of agroindustrial wastewaters under mesophilic conditions in a two-stage process , 2009 .

[21]  I. Angelidaki,et al.  Serial CSTR digester configuration for improving biogas production from manure. , 2009, Water research.

[22]  B. Ahring,et al.  Increase of anaerobic degradation of particulate organic matter in full-scale biogas plants by mechanical maceration. , 2000, Water science and technology : a journal of the International Association on Water Pollution Research.

[23]  B. Mattiasson,et al.  Optimization of sulphide production in an anaerobic continuous biofilm process with sulphate reducing bacteria , 1997, Biotechnology Letters.

[24]  D. Totzke Tapping the potential of codigestion. , 2009 .