A study of the 2013 Western European issue of aflatoxin contamination of maize from the Balkan area

In March 2013 a large shipment of maize, intended for feed was subject of an alert in the Rapid Alert System for Food and Feed of the European Commission (EC) because the aflatoxin B1 (AFB1) level in the load exceeded the EC regulated maximum level of 20 μg/kg. Since the shipment had passed import controls and was already distributed (mainly to German farms), a massive recall followed. The aim of the current study was to investigate questions, raised by authorities and industry, related to the effectivity of EU sampling procedures, the influence of sample homogenisation procedures and sample storage conditions on the test results, and fungal identification as unexpected mycotoxins were identified during this study. The Netherlands Food and Consumer Product Safety Authority seized a shipload of maize in July 2013, suspected to be contaminated with AFB1. The shipload was sampled according to the 2009 and 2013 EC Sampling Regulations to compare the outcomes of both sampling protocols. Mycotoxin analysis of t...

[1]  S. Kocsubé,et al.  Black Aspergilli and fumonisin contamination in onions purchased in Hungary , 2012 .

[2]  T. Schatzki,et al.  Sample comminution for mycotoxin analysis: Dry milling or slurry mixing? , 2006, Food additives and contaminants.

[3]  S. Kocsubé,et al.  Molecular Identification and Antifungal Susceptibilities of Black Aspergillus Isolates from Otomycosis Cases in Hungary , 2012, Mycopathologia.

[4]  P. Cotty,et al.  Influences of climate on aflatoxin producing fungi and aflatoxin contamination. , 2007, International journal of food microbiology.

[5]  M Miraglia,et al.  The role of sampling in mycotoxin contamination: An holistic view , 2005, Food additives and contaminants.

[6]  T B Whitaker,et al.  Sampling Foods for Mycotoxins , 2006, Food additives and contaminants.

[7]  P. Markaki,et al.  Study of the Ochratoxin A effect on Aspergillus parasiticus growth and aflatoxin B1 production. , 2008, Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association.

[8]  T. Whitaker,et al.  Testing shelled corn for aflatoxin, Part II: modeling the observed distribution of aflatoxin test results. , 2000, Journal of AOAC International.

[9]  J. Frisvad,et al.  New ochratoxin A producing species of Aspergillus section Circumdati , 2004 .

[10]  J. Frisvad,et al.  Ochratoxin production and taxonomy of the yellow aspergilli (Aspergillus section Circumdati) , 2014, Studies in mycology.

[11]  J. Frisvad,et al.  A reappraisal of fungi producing aflatoxins , 2009 .

[12]  T B Whitaker,et al.  Design of sampling plans for mycotoxins in foods and feeds. , 1995, Natural toxins.

[13]  G. Payne,et al.  Predicting aflatoxin and fumonisin in shelled corn lots using poor-quality grade components. , 2006, Journal of AOAC International.

[14]  J. Frisvad,et al.  ASPERGILLUS LUCHUENSIS , AN INDUSTRIALLY IMPORTANT BLACK ASPERGILLUS IN EAST ASIA , 2013, PloS one.

[15]  J. W. Dickens,et al.  Protocols for surveys, sampling, post-collection handling, and analysis of grain samples involved in mycotoxin problems. , 1980, Journal - Association of Official Analytical Chemists.

[16]  M. Vieira,et al.  Detection and quantification of Aspergillus westerdijkiae in coffee beans based on selective amplification of β-tubulin gene by using real-time PCR , 2007 .

[17]  N. Magan,et al.  The production of aflatoxin B1 or G1 by Aspergillus parasiticus at various combinations of temperature and water activity is related to the ratio of aflS to aflR expression , 2010, Mycotoxin Research.

[18]  Jonathan W. DeVries,et al.  Mycotoxins and Food Safety , 2002, Advances in Experimental Medicine and Biology.

[19]  Thomas B. Whitaker,et al.  Standardisation of mycotoxin sampling procedures: an urgent necessity , 2003 .

[20]  T B Whitaker,et al.  Testing shelled corn for aflatoxin, Part I: estimation of variance components. , 2000, Journal of AOAC International.

[21]  T. Whitaker,et al.  Testing shelled corn for aflatoxin, Part III: evaluating the performance of aflatoxin sampling plans. , 2000, Journal of AOAC International.

[22]  H. J. van der Fels-Klerx,et al.  A Dutch field survey on fungal infection and mycotoxin concentrations in maize , 2012, Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment.

[23]  T. Whitaker,et al.  Investigations of the problems of assessing aflatoxin levels in peanuts. , 1998, Biometrics.

[24]  J. Frisvad,et al.  Penicillium verrucosum in wheat and barley indicates presence of ochratoxin A , 2003, Journal of applied microbiology.

[25]  J. Frisvad,et al.  Food and indoor fungi , 2010 .

[26]  Maren A. Klich,et al.  Environmental and developmental factors influencing aflatoxin production by Aspergillus flavus and Aspergillus parasiticus , 2007, Mycoscience.

[27]  Bojana Šarić,et al.  Natural occurrence of aflatoxins in maize harvested in Serbia during 2009–2012 , 2013 .

[28]  M. Klich.  Aspergillus flavus: the major producer of aflatoxin. , 2007, Molecular plant pathology.

[29]  D. Bolonhezi,et al.  Aflatoxins, ochratoxin A and zearalenone in Brazilian corn cultivars , 2001 .