Membrane Hyperpolarization Is Not Required for Sustained Muscarinic Agonist‐Induced Increases in Intracellular Ca2+ in Arteriolar Endothelial Cells

Objective: Hyperpolarization modulates Ca2+ influx during agonist stimulation in many endothelial cells, but the effects of hyperpolarization on Ca2+ influx in freshly isolated arteriolar endothelial cells are unknown. Therefore, the purpose of the present study was to characterize agonist‐induced Ca2+ transients in freshly isolated arteriolar endothelial cells and to test the hypothesis that membrane hyperpolarization augments agonist‐induced Ca2+ influx into these cells.

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