A Trial of Reducing Adhesion Formation in a Uterine Horn Model

For the prevention of postoperative adhesion formation, one of the most common causes of infertility, none of the adjuvants had been proven uniformly effective. In this study we evaluated the effectiveness of sodium carboxymethylcellulose (SCMC) and disodium cromoglycate (DSCG) in postoperative adhesion prevention in a rat uterine horn model. Thirty female Sprague-Dawley rats were used. After uterine horn abrasion, in 10 rats 10 ml 0.9% saline, in 10 rats 10 ml of 2% SCMC, and in 10 rats 10 ml DSCG were administered intraperitoneally. Two weeks later, all animals were sacrificed and adhesion formation was assessed. All the pieces of the peritoneum biopsies were stained with Luna’s mast cell stain to assess the mast cell degranulation. The mean adhesion scores were 2.1, 2.0 and 1.5 for saline, SCMC and DSCG groups respectively. There were no significant differences among all groups. In the pathologic examination, mast cell degranulation was less in the DSCG group than the other groups.

[1]  J. Rock,et al.  Expanded polytetrafluoroethylene (Gore-Tex Surgical Membrane) is superior to oxidized regenerated cellulose (Interceed TC7+) in preventing adhesions. , 1995, Fertility and sterility.

[2]  H. Sax,et al.  Evaluation of a carboxymethylcellulose sponge for prevention of postoperative adhesions. , 1995, American journal of surgery.

[3]  J. Thompson,et al.  Experimental adhesion prophylaxis with recombinant tissue plasminogen activator. , 1994, Annals of the Royal College of Surgeons of England.

[4]  Heidrick Gw,et al.  Efficacy of intraperitoneal sodium carboxymethylcellulose in preventing postoperative adhesion formation. , 1994 .

[5]  K. O'Hair,et al.  Combined effects of tissue plasminogen activator and carboxymethylcellulose on adhesion reformation in rabbits. , 1994, International journal of fertility and menopausal studies.

[6]  Y. Şahin,et al.  Synergistic effects of carboxymethylcellulose and low molecular weight heparin in reducing adhesion formation in the rat uterine horn model , 1994, Acta obstetricia et gynecologica Scandinavica.

[7]  M. Morgan,et al.  Efficacy of intraperitoneal sodium carboxymethylcellulose in preventing postoperative adhesion formation. , 1994, The Journal of reproductive medicine.

[8]  I. Chaudry,et al.  Prevention of peritoneal adhesions by administration of sodium carboxymethyl cellulose and oral vitamin E. , 1993, Surgery.

[9]  E. Goldberg,et al.  Prevention of postoperative abdominal adhesions by tissue precoating with polymer solutions. , 1993, The Journal of surgical research.

[10]  J. Langer,et al.  Role of mast cells in peritoneal adhesion formation. , 1993, American journal of surgery.

[11]  M. Diamond,et al.  Synergistic effects of INTERCEED(TC7) and heparin in reducing adhesion formation in the rabbit uterine horn model. , 1991, Fertility and sterility.

[12]  B. Jakschik,et al.  The importance of mast cells for the neutrophil influx in immune complex-induced peritonitis in mice. , 1990, Journal of immunology.

[13]  C. Sueldo,et al.  Reduction of primary postoperative adhesion formation under calcium channel blockade in the rabbit. , 1990, The Journal of surgical research.

[14]  Jansen Rp.  Failure of peritoneal irrigation with heparin during pelvic operations upon young women to reduce adhesions. , 1988 .

[15]  R. Jansen.  Failure of peritoneal irrigation with heparin during pelvic operations upon young women to reduce adhesions. , 1988, Surgery, gynecology & obstetrics.

[16]  M. Diamond,et al.  Assessment of carboxymethylcellulose and 32% dextran 70 for prevention of adhesions in a rabbit uterine horn model. , 1988, International journal of fertility.

[17]  A. Decherney,et al.  Chondroitin sulphate. A new intraperitoneal treatment for postoperative adhesion prevention in the rabbit. , 1987, The Journal of reproductive medicine.

[18]  M. Diamond,et al.  Pathogenesis of adhesion formation/reformation: Application to reproductive pelvic surgery , 1987, Microsurgery.

[19]  G. Serour,et al.  Adhesion prevention in the rabbit with sodium carboxymethylcellulose solutions. , 1986, American journal of obstetrics and gynecology.

[20]  G. Holtz.  Current use of ancillary modalities for adhesion prevention. , 1985, Fertility and sterility.

[21]  R. Ahokas,et al.  Adhesion prevention by solutions of sodium carboxymethylcellulose in the rat. I. , 1984, Fertility and sterility.

[22]  M. Soules,et al.  Reduction of postoperative pelvic adhesions with intraperitoneal 32% dextran 70: a prospective, randomized clinical trial. , 1983, Fertility and sterility.

[23]  K. Swolin,et al.  POSTOPERATIVE ADHESIONS: ETIOLOGY, PREVENTION, AND THERAPY , 1980, Clinical obstetrics and gynecology.

[24]  R. Bronson,et al.  Lysis of periadnexal adhesions for correction of infertility. , 1977, Fertility and sterility.

[25]  R. Buckman,et al.  A physiologic basis for the adhesion-free healing of deperitonealized surfaces. , 1976, The Journal of surgical research.

[26]  R. Gross,et al.  Prevention of Postoperative Intestinal Adhesions with Combined Promethazine and Dexamethasone Therapy: Experimental and Clinical Studies , 1966, Annals of surgery.

[27]  R. Lillie,et al.  Manual of Histologic and Special Staining Techniques , 1958 .