Non-covalent and covalent protein labeling in two-dimensional gel electrophoresis.

[1]  V. Santoni,et al.  Detergents and chaotropes for protein solubilization before two-dimensional electrophoresis. , 2009, Methods in molecular biology.

[2]  G. Leuba,et al.  Serial protein labeling with infrared maleimide dyes to identify cysteine modifications. , 2008, Journal of proteomics.

[3]  R. Cohen,et al.  Thiol oxidation in signaling and response to stress: detection and quantification of physiological and pathophysiological thiol modifications. , 2007, Free radical biology & medicine.

[4]  B. Riederer,et al.  Differential protein labeling with thiol‐reactive infrared DY‐680 and DY‐780 maleimides and analysis by two‐dimensional gel electrophoresis , 2007, Proteomics.

[5]  Michael Olivier,et al.  Improved method for the analysis of membrane proteins by mass spectrometry. , 2007, Physiological genomics.

[6]  N. Cueille,et al.  Microtubule-associated protein 1B binds glyceraldehyde-3-phosphate dehydrogenase. , 2007, Journal of proteome research.

[7]  B. Riederer,et al.  Microtubule-associated protein 1B, a growth-associated and phosphorylated scaffold protein , 2007, Brain Research Bulletin.

[8]  H. Mock,et al.  Quantitative proteome analysis of barley seeds using ruthenium(II)-tris-(bathophenanthroline-disulphonate) staining. , 2007, Journal of proteome research.

[9]  Zhen Liu,et al.  Evaluation of the application of sodium deoxycholate to proteomic analysis of rat hippocampal plasma membrane. , 2006, Journal of proteome research.

[10]  B. Winblad,et al.  Involvement of glutaredoxin-1 and thioredoxin-1 in β-amyloid toxicity and Alzheimer's disease , 2006, Cell Death and Differentiation.

[11]  S. Hirohashi,et al.  Toward a comprehensive quantitative proteome database: protein expression map of lymphoid neoplasms by 2‐D DIGE and MS , 2006, Proteomics.

[12]  Sergio Ciordia,et al.  Contributions of advanced proteomics technologies to cancer diagnosis , 2006, Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico.

[13]  K. Dietz,et al.  Concepts and approaches towards understanding the cellular redox proteome. , 2006, Plant biology.

[14]  Karl Mechtler,et al.  Phosphoproteomics strategies for the functional analysis of signal transduction , 2006, Proteomics.

[15]  S. Hemby,et al.  Quantitation in two‐dimensional fluorescence difference gel electrophoresis: Effect of protein fixation , 2006, Electrophoresis.

[16]  D. Harman.  Free Radical Theory of Aging: An Update , 2006, Annals of the New York Academy of Sciences.

[17]  F. Nomura.  [Clinical proteomics in laboratory medicine]. , 2006, Rinsho byori. The Japanese journal of clinical pathology.

[18]  Marianne Fillet,et al.  High‐sensitivity staining of proteins for one‐ and two‐dimensional gel electrophoresis using post migration covalent staining with a ruthenium fluorophore , 2006, Electrophoresis.

[19]  Jeffrey N Keller,et al.  Proteasome regulation of oxidative stress in aging and age-related diseases of the CNS. , 2006, Antioxidants & redox signaling.

[20]  I. Alafuzoff,et al.  Oxidative modification of proteins in the frontal cortex of Alzheimer's disease brain , 2006, Neurobiology of Aging.

[21]  C. A. Blake.  Physiological Proteomics: Cells, Organs, Biological Fluids, and Biomarkers , 2005, Experimental biology and medicine.

[22]  Guan-Tarn Huang,et al.  Identification of human hepatocellular carcinoma-related biomarkers by two-dimensional difference gel electrophoresis and mass spectrometry. , 2005, Journal of proteome research.

[23]  T. Montine,et al.  Proteomic determination of widespread detergent insolubility, including Aβ but not tau, early in the pathogenesis of Alzheimer's disease , 2005, FASEB journal : official publication of the Federation of American Societies for Experimental Biology.

[24]  T. Franz,et al.  Threshold values for detergents in protein and peptide samples for mass spectrometry. , 2005, Rapid communications in mass spectrometry : RCM.

[25]  Richard D. LeDuc,et al.  Mass spectrometric peptide fingerprinting of proteins after Western blotting on polyvinylidene fluoride and enhanced chemiluminescence detection. , 2005, Journal of proteome research.

[26]  R. Caprioli,et al.  Nonacid cleavable detergents applied to MALDI mass spectrometry profiling of whole cells. , 2005, Journal of mass spectrometry : JMS.

[27]  Jian Cai,et al.  Proteomic identification of oxidatively modified retinal proteins in a chronic pressure-induced rat model of glaucoma. , 2005, Investigative ophthalmology & visual science.

[28]  M. Waterfield,et al.  Proteomic analysis of redox‐ and ErbB2‐dependent changes in mammary luminal epithelial cells using cysteine‐ and lysine‐labelling two‐dimensional difference gel electrophoresis , 2005, Proteomics.

[29]  T. Toda,et al.  Age‐dependent variations of cell response to oxidative stress: Proteomic approach to protein expression and phosphorylation , 2005, Electrophoresis.

[30]  C. Huck,et al.  Quantitative detection of phosphoproteins by combination of two‐dimensional difference gel electrophoresis and phosphospecific fluorescent staining , 2005, Electrophoresis.

[31]  R. Caprioli,et al.  Combination detergent/MALDI matrix: functional cleavable detergents for mass spectrometry. , 2005, Analytical chemistry.

[32]  A. Nel,et al.  Use of a fluorescent phosphoprotein dye to characterize oxidative stress‐induced signaling pathway components in macrophage and epithelial cultures exposed to diesel exhaust particle chemicals , 2005, Electrophoresis.

[33]  Hirokazu Yamaguchi,et al.  Saturation labeling with cysteine‐reactive cyanine fluorescent dyes provides increased sensitivity for protein expression profiling of laser‐microdissected clinical specimens , 2005, Proteomics.

[34]  L. Brady,et al.  Solubilization of cellular membrane proteins from Streptococcus mutans for two‐dimensional gel electrophoresis , 2005, Electrophoresis.

[35]  A. Heck,et al.  Double Standards in Quantitative Proteomics , 2005, Molecular & Cellular Proteomics.

[36]  Iain D G Campuzano,et al.  Proteomic Analysis of in Vivo Phosphorylated Synaptic Proteins* , 2005, Journal of Biological Chemistry.

[37]  Fiona M McCarthy,et al.  Differential detergent fractionation for non-electrophoretic eukaryote cell proteomics. , 2005, Journal of proteome research.

[38]  Wayne F. Patton,et al.  Multiplexed fluorescence detection of phosphorylation, glycosylation, and total protein in the proteomic analysis of breast cancer refractoriness , 2004, Proteomics.

[39]  R. Maccioni,et al.  Biological markers of Alzheimer's disease and mild cognitive impairment. , 2004, Current Alzheimer research.

[40]  L. Landino,et al.  Redox modulation of tau and microtubule-associated protein-2 by the glutathione/glutaredoxin reductase system. , 2004, Biochemical and biophysical research communications.

[41]  G. Barja.  Free radicals and aging , 2004, Trends in Neurosciences.

[42]  L. Landino,et al.  Cysteine Oxidation of Tau and Microtubule-associated Protein-2 by Peroxynitrite , 2004, Journal of Biological Chemistry.

[43]  R. Aggeler,et al.  Focused proteomics: Monoclonal antibody‐based isolation of the oxidative phosphorylation machinery and detection of phosphoproteins using a fluorescent phosphoprotein gel stain , 2004, Electrophoresis.

[44]  Alexander Lazarev,et al.  Comparison of fluorescent stains: Relative photostability and differential staining of proteins in two‐dimensional gels , 2004, Electrophoresis.

[45]  Wayne F. Patton,et al.  Characterization of dynamic and steady‐state protein phosphorylation using a fluorescent phosphoprotein gel stain and mass spectrometry , 2004, Electrophoresis.

[46]  Wayne F. Patton,et al.  Optimized conditions for diluting and reusing a fluorescent protein gel stain , 2004, Electrophoresis.

[47]  François Chevalier,et al.  Proteomic capacity of recent fluorescent dyes for protein staining. , 2004, Phytochemistry.

[48]  David P. Kreil,et al.  Determining a significant change in protein expression with DeCyder™ during a pair‐wise comparison using two‐dimensional difference gel electrophoresis , 2004, Proteomics.

[49]  T. Ozben,et al.  Reactive oxygen and nitrogen species in Alzheimer's disease. , 2004, Current Alzheimer research.

[50]  H. Chae,et al.  Fast visible dye staining of proteins in one‐ and two‐dimensional sodium dodecyl sulfate‐polyacrylamide gels compatible with matrix assisted laser desorption/ionization‐mass spectrometry , 2004, Electrophoresis.

[51]  L. Landino,et al.  Modulation of the redox state of tubulin by the glutathione/glutaredoxin reductase system. , 2004, Biochemical and biophysical research communications.

[52]  Thomas P Conrads,et al.  A detergent‐ and cyanogen bromide‐free method for integral membrane proteomics: Application to Halobacterium purple membranes and the human epidermal membrane proteome , 2004, Proteomics.

[53]  Peter Karuso,et al.  A fluorescent natural product for ultra sensitive detection of proteins in one‐dimensional and two‐dimensional gel electrophoresis , 2003, Proteomics.

[54]  M. Lagarde,et al.  Protein and lipid analysis of detergent-resistant membranes isolated from bovine kidney. , 2003, Biochimie.

[55]  M. Buttner,et al.  Thiol-based regulatory switches. , 2003, Annual review of genetics.

[56]  Kaj Blennow,et al.  Proteomic studies of potential cerebrospinal fluid protein markers for Alzheimer's disease. , 2003, Brain research. Molecular brain research.

[57]  Kelvin H. Lee,et al.  Comparison of automated in‐gel digest methods for femtomole level samples , 2003, Electrophoresis.

[58]  B. Riederer,et al.  Sample preparation for two‐dimensional gel electrophoresis , 2003, Proteomics.

[59]  Wayne F. Patton,et al.  A sensitive two‐color electrophoretic mobility shift assay for detecting both nucleic acids and protein in gels , 2003, Proteomics.

[60]  Wayne F. Patton,et al.  Global quantitative phosphoprotein analysis using Multiplexed Proteomics technology , 2003, Proteomics.

[61]  Robert Tonge,et al.  Evaluation of saturation labelling two‐dimensional difference gel electrophoresis fluorescent dyes , 2003, Proteomics.

[62]  Alvydas Mikulskis,et al.  High‐content proteomics: Fluorescence multiplexing using an integrated, high‐sensitivity, multiwavelength charge‐coupled device imaging system , 2003, Proteomics.

[63]  V. Santoni,et al.  Evaluation of nonionic and zwitterionic detergents as membrane protein solubilizers in two‐dimensional electrophoresis , 2003, Proteomics.

[64]  T. Rabilloud,et al.  Structure‐efficiency relationships of zwitterionic detergents as protein solubilizers in two‐dimensional electrophoresis , 2003, Proteomics.

[65]  P. Hornbeck,et al.  Phosphoprotein Analysis Using Antibodies Broadly Reactive against Phosphorylated Motifs* , 2002, The Journal of Biological Chemistry.

[66]  I. Alafuzoff,et al.  Proteomic analysis of protein oxidation in Alzheimer's disease brain , 2002, Electrophoresis.

[67]  D. Butterfield,et al.  Proteomic identification of oxidatively modified proteins in Alzheimer's disease brain. Part II: dihydropyrimidinase‐related protein 2, α‐enolase and heat shock cognate 71 , 2002, Journal of neurochemistry.

[68]  C. Winterbourn,et al.  Detection of oxidant sensitive thiol proteins by fluorescence labeling and two‐dimensional electrophoresis , 2002, Proteomics.

[69]  Petra Schwille,et al.  Probing the endocytic pathway in live cells using dual-color fluorescence cross-correlation analysis. , 2002, Biophysical journal.

[70]  Wayne F. Patton,et al.  Detection technologies in proteome analysis. , 2002, Journal of chromatography. B, Analytical technologies in the biomedical and life sciences.

[71]  D. Giustarini,et al.  Methionine oxidation as a major cause of the functional impairment of oxidized actin. , 2002, Free radical biology & medicine.

[72]  Wayne F. Patton,et al.  An improved formulation of SYPRO Ruby protein gel stain: Comparison with the original formulation and with a ruthenium II tris (bathophenanthroline disulfonate) formulation , 2002, Proteomics.

[73]  Kaj Blennow,et al.  Proteome analysis of cerebrospinal fluid proteins in Alzheimer patients , 2002, Neuroreport.

[74]  H. Boucherie,et al.  Differential gel exposure, a new methodology for the two‐dimensional comparison of protein samples , 2002, Proteomics.

[75]  B. Violand,et al.  Development of improved cell lysis, solubilization and imaging approaches for proteomic analyses , 2002, Proteomics.

[76]  J. Klose,et al.  Fluorescent dual colour 2D-protein gel electrophoresis for rapid detection of differences in protein pattern with standard image analysis software. , 2001, International journal of molecular medicine.

[77]  Wayne F. Patton,et al.  Rapid and simple single nanogram detection of glycoproteins in polyacrylamide gels and on electroblots , 2001, Proteomics.

[78]  T. Rabilloud,et al.  A comparison between Sypro Ruby and ruthenium II tris (bathophenanthroline disulfonate) as fluorescent stains for protein detection in gels , 2001, Proteomics.

[79]  J. A. Carroll,et al.  Mass spectrometry compatibility of two‐dimensional gel protein stains , 2001, Electrophoresis.

[80]  Matthew Davison,et al.  Validation and development of fluorescence two‐dimensional differential gel electrophoresis proteomics technology , 2001, Proteomics.

[81]  R. Levine,et al.  Identification of Oxidant-Sensitive Proteins: TNF-α Induces Protein Glutathiolation , 2000 .

[82]  Wayne F. Patton,et al.  Background‐free, high sensitivity staining of proteins in one‐ and two‐dimensional sodium dodecyl sulfate‐polyacrylamide gels using a luminescent ruthenium complex , 2000, Electrophoresis.

[83]  C. Desponts,et al.  A general method for the rapid characterization of tyrosine‐phosphorylated proteins by mini two‐dimensional gel electrophoresis , 2000, Electrophoresis.

[84]  Wayne F. Patton.  A thousand points of light: The application of fluorescence detection technologies to two‐dimensional gel electrophoresis and proteomics , 2000, Electrophoresis.

[85]  Wayne F. Patton,et al.  Ultrasensitive fluorescence protein detection in isoelectric focusing gels using a ruthenium metal chelate stain , 2000, Electrophoresis.

[86]  Wayne F. Patton,et al.  Fluorescence detection of proteins in sodium dodecyl sulfate‐polyacrylamide gels using environmentally benign, nonfixative, saline solution , 2000, Electrophoresis.

[87]  Wayne F. Patton,et al.  A luminescent ruthenium complex for ultrasensitive detection of proteins immobilized on membrane supports. , 1999, Analytical biochemistry.

[88]  F. J. Díez-Guerra,et al.  Microscale purification of proteins exhibiting anomalous electrophoretic migration: application to the analysis of GAP-43 phosphorylation. , 1999, Analytical biochemistry.

[89]  M. Jeannot,et al.  Observation of sodium gel-induced protein modifications in dodecylsulfate polyacrylamide gel electrophoresis and its implications for accurate molecular weight determination of gel-separated proteins by matrix-assisted laser desorption ionization time-of-flight mass spectrometry , 1999 .

[90]  K. Blennow,et al.  Removal of sodium dodecyl sulfate from protein samples prior to matrix-assisted laser desorption/ionization mass spectrometry. , 1999, Rapid communications in mass spectrometry : RCM.

[91]  M. Glocker,et al.  APPROACHES TO THE CHARACTERIZATION OF MEMBRANE CHANNEL PROTEINS (PORINS) BY UV MALDI-MS , 1997 .

[92]  E. Stadtman,et al.  Protein Oxidation in Aging, Disease, and Oxidative Stress* , 1997, The Journal of Biological Chemistry.

[93]  G. Bruin,et al.  Influence of solvents and detergents on matrix-assisted laser desorption/ionization mass spectrometry measurements of proteins and oligonucleotides. , 1997, Rapid communications in mass spectrometry : RCM.

[94]  R. Haugland,et al.  Applications of SYPRO orange and SYPRO red protein gel stains. , 1996, Analytical biochemistry.

[95]  L. Jones,et al.  SYPRO orange and SYPRO red protein gel stains: one-step fluorescent staining of denaturing gels for detection of nanogram levels of protein. , 1996, Analytical biochemistry.

[96]  Franz Hillenkamp,et al.  Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) of membrane proteins and non-covalent complexes , 1995 .

[97]  S. Vandenberg,et al.  Alterations of annexin expression in pathological neuronal and glial reactions. Immunohistochemical localization of annexins I, II (p36 and p11 subunits), IV, and VI in the human hippocampus. , 1994, The American journal of pathology.

[98]  G. W. Rafter.  Plasma thiols, copper and rheumatoid arthritis. , 1994, Medical hypotheses.

[99]  C. Merril,et al.  Ultrasensitive stain for proteins in polyacrylamide gels shows regional variation in cerebrospinal fluid proteins. , 1981, Science.

[100]  A. Datyner,et al.  A new staining method for the assay of proteins on polyacrylamide gels. , 1973, Analytical biochemistry.

[101]  C. Brinkworth,et al.  The effect of Tween80 on signal intensity of intact proteins by MALDI time-of-flight mass spectrometry , 2007, Journal of the American Society for Mass Spectrometry.

[102]  S. Solomon,et al.  Functional characterization of two‐dimensional gel‐separated proteins using sequential staining , 2005, Electrophoresis.

[103]  B. Lanne,et al.  Protein staining influences the quality of mass spectra obtained by peptide mass fingerprinting after separation on 2-d gels. A comparison of staining with coomassie brilliant blue and sypro ruby. , 2005, Journal of proteome research.

[104]  B. Riederer,et al.  Thiol-reactive protein labeling of brain proteins with infrared DY-680 and DY-782 maleimides , 2004 .

[105]  Andrew J. Link.  Autoradiography of 2-D gels. , 1999, Methods in molecular biology.

[106]  M. Ünlü,et al.  Difference gel electrophoresis. A single gel method for detecting changes in protein extracts , 1997, Electrophoresis.

[107]  Richard P. Haugland,et al.  Handbook of fluorescent probes and research chemicals , 1996 .

[108]  P. Dráber.  Quantification of proteins in sample buffer for sodium dodecyl sulfate‐polyacrylamide gel electrophoresis using colloidal silver , 1991, Electrophoresis.

[109]  V. Neuhoff,et al.  Improved staining of proteins in polyacrylamide gels including isoelectric focusing gels with clear background at nanogram sensitivity using Coomassie Brilliant Blue G‐250 and R‐250 , 1988, Electrophoresis.

[110]  C. Mackay,et al.  Rapid imaging, using a cooled charge‐coupled‐device, of fluorescent two‐dimensional polyacrylamide gels produced by labelling proteins in the first‐dimensional isoelectric focusing gel with the fluorophore 2‐methoxy‐2,4‐diphenyl‐3(2H)furanone , 1988, Electrophoresis.

[111]  Merril Cr,et al.  'Catalysts' for polyacrylamide gel polymerization and detection of proteins by silver staining. , 1988 .

[112]  H. Gross,et al.  Improved silver staining of plant proteins, RNA and DNA in polyacrylamide gels , 1987 .

[113]  R. Webster,et al.  Two new staining procedures for quantitative estimation of proteins on electrophoretic strips. , 1963, Biochimica et biophysica acta.

[114]  T. Montine,et al.  The FASEB Journal express article 10.1096/fj.04-3210fje. Published online March 3, 2005. ©2005 FASEB , 2022 .