Antioxidant Activity and Phenolic Content of Sonication- and Maceration-Assisted Ethanol and Acetone Extracts of Cymbopogon citratus Leaves

Cymbopogon citratus is a medicinal and well-known aromatic plant which is usually used as a substitute for green-tea with extraordinary phytomedicinal potential. It is of great importance because it offers several promising health effects. The objective of the study was to investigate the antioxidant activity and total phenolic content of lemongrass leaves extracted by maceration and ultrasound-assisted extraction techniques. Different concentrations of both acetone and ethanol (50% and 70%) as solvents were tested for their radical scavenging activity against the stable free radical DPPH in quantization using a spectrophotometric assay. The ultrasound-assisted extraction technique had advantageous results in the polyphenols extraction yield (26.68%), TPC (61 mg GAE/g of extract) and DPPH (73.85%) compared to the maceration technique. Overall, the results were significant from 50% ethanol and the DPPH results were more significant from 70% ethanol than other concentrations. It is concluded that the ultrasound-assisted extraction is a better extraction technique for polyphenols than other conventional techniques.

[1]  J. Uddin,et al.  Extraction and Evaluation of the Antimicrobial Activity of Polyphenols from Banana Peels Employing Different Extraction Techniques , 2022, Separations.

[2]  Ume Roobab,et al.  Sonication, a Potential Technique for Extraction of Phytoconstituents: A Systematic Review , 2021, Processes.

[3]  Rai Naveed Arshad,et al.  A Critical Review on Pulsed Electric Field: A Novel Technology for the Extraction of Phytoconstituents , 2021, Molecules.

[4]  S. Akhtar,et al.  Heterocyclic Aromatic Amines in Meat: Formation, Isolation, Risk Assessment, and Inhibitory Effect of Plant Extracts , 2021, Foods.

[5]  S. Mahmood,et al.  A comprehensive review on phytochemistry, bioactivity and medicinal value of bioactive compounds of pomegranate (Punica granatum) , 2021, Advances in Traditional Medicine.

[6]  A. Mehmood,et al.  Sonication and Microwave Processing of Phalsa Drink: A Synergistic Approach , 2021, International Journal of Fruit Science.

[7]  S. Machmudah,et al.  Recovery and solubility of flavonoid and phenolic contents from Arachis Hypogea in supercritical carbon dioxide assisted by ethanol as cosolvent , 2020 .

[8]  S. Mahmood,et al.  Extraction of Polyphenols from Apple and Pomegranate Peels Employing Different Extraction Techniques for the Development of Functional Date Bars , 2020 .

[9]  S. Mahmood,et al.  A Comprehensive Review on Nutritional Value, Medicinal Uses, and Processing of Banana , 2020, Food Reviews International.

[10]  A. Galadima,et al.  Assessment of Bioenergy Potential of Lemon Grass , 2019, Archives of Current Research International.

[11]  Rana Muhammad Aadil,et al.  Thermosonication: a potential technique that influences the quality of grapefruit juice , 2015 .

[12]  Xiaoxiong Zeng,et al.  Study on combined effects of blanching and sonication on different quality parameters of carrot juice , 2014, International journal of food sciences and nutrition.

[13]  J. Patel,et al.  Antimicrobial activity of lemongrass oil against Salmonella enterica on organic leafy greens , 2012, Journal of applied microbiology.

[14]  A. Witkowska,et al.  Antioxidant Potential and Polyphenol Content of Selected Food , 2011 .

[15]  A. Segura‐Carretero,et al.  Phenolic-Compound-Extraction Systems for Fruit and Vegetable Samples , 2010, Molecules.

[16]  Russell J. Mumper,et al.  Plant Phenolics: Extraction, Analysis and Their Antioxidant and Anticancer Properties , 2010, Molecules.

[17]  M. Tabernero,et al.  Bioavailability of Phenolic Antioxidants Associated with Dietary Fiber: Plasma Antioxidant Capacity After Acute and Long-Term Intake in Humans , 2009, Plant foods for human nutrition.

[18]  P. Geraldine,et al.  In-vitro antioxidant activities of an ethanolic extract of the oyster mushroom, Pleurotus ostreatus , 2009 .

[19]  S. Arntfield,et al.  A comparative study on the phenolic acids identified and quantified in dry beans using HPLC as affected by different extraction and hydrolysis methods. , 2009 .

[20]  Raymond Mawson,et al.  APPLICATIONS AND OPPORTUNITIES FOR ULTRASOUND ASSISTED EXTRACTION IN THE FOOD INDUSTRY-A REVIEW , 2008 .

[21]  Noppawan Phumala Morales,et al.  The in vitro and ex vivo antioxidant properties, hypolipidaemic and antiatherosclerotic activities of water extract of Moringa oleifera Lam. leaves. , 2008, Journal of ethnopharmacology.

[22]  T. G. Toschi,et al.  Phenol content related to antioxidant and antimicrobial activities of Passiflora spp. extracts , 2006 .

[23]  A. Gilani,et al.  Isolation and structure elucidation of new nitrile and mustard oil glycosides from Moringa oleifera and their effect on blood pressure. , 1994, Journal of natural products.

[24]  A. Mishra,et al.  Evaluation of some essential oils for their toxicity against fungi causing deterioration of stored food commodities , 1994, Applied and environmental microbiology.

[25]  Effect of lemongrass powder on proximate and phytochemical content of herbal cookies , 2017 .

[26]  S. Falah,et al.  Potential of lemongrass leaves extract (Cymbopogon citratus) as prevention for oil oxidation , 2015 .

[27]  R. Rahman,et al.  Ultrasound-assisted extraction of valuable compounds from winter melon (Benincasa hispida) seeds , 2013 .

[28]  S. Adhikari,et al.  Assessment of genetic diversity of certain Indian elite clones of Cymbopogon species through RAPD analysis , 2013 .

[29]  M. Mirghani,et al.  Bioactivity analysis of lemongrass (Cymbopogan citratus) essential oil. , 2012 .

[30]  Jalan Menara Gading,et al.  ANTIOXIDANT CAPACITY AND TOTAL PHENOLIC CONTENT OF LEMONGRASS (CYMBOPOGON CITRATUS) LEAVE , 2012 .

[31]  T. Marar,et al.  Comparative Analysis of Phytochemical Profile and Antioxidant Activity of Some , 2012 .

[32]  V. Nambiar.  Potential Functions of Lemon Grass Cymbopogon citratus in Health and Disease , 2012 .

[33]  Gagan Shah,et al.  Scientific basis for the therapeutic use of Cymbopogon citratus, stapf (Lemon grass) , 2011, Journal of advanced pharmaceutical technology & research.

[34]  K. Ravinder,et al.  Pharmacognostical Investigation of Cymbopogon citratus (DC) Stapf , 2010 .

[35]  O. Oloyede.  Chemical profile and antimicrobial activity of Cymbopogon citratus leaves. , 2009 .

[36]  S. B. Agrawal,et al.  Evaluation of changes in oil cells and composition of essential oil in lemongrass (Cymbopogon citratus (D.C.) Stapf.) due to supplemental ultraviolet-B irradiation. , 2009 .

[37]  J. Kumar,et al.  GENETIC DIVERSITY ANALYSIS IN CYMBOPOGON SPECIES USING DNA MARKERS , 2009 .

[38]  R. Lamuela-Raventós,et al.  Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-Ciocalteu reagent , 1999 .