Heterotopic Transplantation of Testes in Newly Hatched Chickens and Subsequent Production of Offspring via Intramagnal Insemination1

Abstract Transplantation of testicular tissue onto the back of immunodeficient nude mice provides a tool to examine testicular development and preserve fertility in mammals. There is no immunodeficient model in birds, but we recently transplanted ovarian tissue between newly hatched chicks from two lines of chickens and produced donor-derived offspring, showing that experimental transplantation is possible in newly hatched chicks. In the present study testicular tissue from newly hatched Barred Plymouth Rock (BPR) chicks was transplanted under the skin of the back, under the skin of the abdomen, or in the abdomen of White Leghorn chicks that had been surgically castrated and immunocompromised. Recipient birds were killed at 10 mo of age. Transplanted tissue was observed in one of five hosts receiving tissue under the skin of the back, two of five hosts receiving tissue under the skin of the abdomen, and three of five chicks with grafts inside the abdominal cavity. In recipients with no regeneration of host testes, testicular transplants grew to the size of normal testes, and histologic analysis showed active spermatogenesis. Subsequent collection of sperm from two successful transplants and surgical insemination of the sperm into the magna of the oviducts of BPR hens resulted in the production of 24 donor-derived chicks. These results demonstrate that the combination of testicular tissue transplantation with intramagnal insemination can produce viable, normal chicks, which could provide a simple approach for the recuperation of live offspring in avian species.

[1]  F. Silversides,et al.  Pure line laying chickens at the Agassiz Research Centre , 2007 .

[2]  F. Silversides,et al.  Offspring produced from orthotopic transplantation of chicken ovaries. , 2007, Poultry science.

[3]  P. Trefil,et al.  Restoration of Spermatogenesis and Male Fertility by Transplantation of Dispersed Testicular Cells in the Chicken1 , 2006, Biology of reproduction.

[4]  J. Han,et al.  A Testis-Mediated Germline Chimera Production Based on Transfer of Chicken Testicular Cells Directly into Heterologous Testes1 , 2006, Biology of reproduction.

[5]  W. Zeng,et al.  Germ cell development in equine testis tissue xenografted into mice. , 2006, Reproduction.

[6]  F. Silversides,et al.  The technique of orthotopic ovarian transplantation in the chicken. , 2006, Poultry science.

[7]  J. Petitte Avian germplasm preservation: embryonic stem cells or primordial germ cells? , 2006, Poultry science.

[8]  R. Dhir,et al.  Limited survival of adult human testicular tissue as ectopic xenograft. , 2006, Human reproduction.

[9]  H. Tournaye,et al.  Spermatogonial survival after grafting human testicular tissue to immunodeficient mice. , 2006, Human reproduction.

[10]  W. Zeng,et al.  Germ cell fate and seminiferous tubule development in bovine testis xenografts. , 2005, Reproduction.

[11]  I. Dobrinski Germ cell transplantation and testis tissue xenografting in domestic animals. , 2005, Animal reproduction science.

[12]  J. Petitte,et al.  Production of germline chimeric chickens following the administration of a busulfan emulsion , 2005, Molecular reproduction and development.

[13]  D. Mclean,et al.  Establishment of Spermatogenesis in Neonatal Bovine Testicular Tissue Following Ectopic Xenografting Varies with Donor Age1 , 2005, Biology of reproduction.

[14]  Marvin Mundry,et al.  CoGrafting of Hamster (Phodopus sungorus) and Marmoset (Callithrix jacchus) Testicular Tissues into Nude Mice Does Not Overcome Blockade of Early Spermatogenic Differentiation in Primate Grafts1 , 2004, Biology of reproduction.

[15]  A. Honaramooz,et al.  Accelerated Maturation of Primate Testis by Xenografting into Mice1 , 2004, Biology of reproduction.

[16]  Harold Jackson,et al.  The testis , 2004, Cancer Chemotherapy and Pharmacology.

[17]  H. Schöler,et al.  Progeny from Sperm Obtained after Ectopic Grafting of Neonatal Mouse Testes1 , 2003, Biology of reproduction.

[18]  A. Honaramooz,et al.  Germ cell transplantation in goats , 2003, Molecular reproduction and development.

[19]  S. Schlatt,et al.  Spermatogenesis and steroidogenesis in mouse, hamster and monkey testicular tissue after cryopreservation and heterotopic grafting to castrated hosts. , 2002, Reproduction.

[20]  H. Schöler,et al.  Sperm from neonatal mammalian testes grafted in mice , 2002, Nature.

[21]  S. Schlatt Germ cell transplantation , 2002, Molecular and Cellular Endocrinology.

[22]  B. Mezquita,et al.  Marked differences between avian and mammalian testicular cells in the heat shock induction and polyadenylation of Hsp70 and ubiquitin transcripts , 1998, FEBS letters.

[23]  J. Kirby,et al.  Determination of testis temperature rhythms and effects of constant light on testicular function in the domestic fowl (Gallus domesticus). , 1997, Biology of reproduction.

[24]  R. Brinster,et al.  Spermatogenesis following male germ-cell transplantation. , 1994, Proceedings of the National Academy of Sciences of the United States of America.

[25]  R. Brinster,et al.  Germline transmission of donor haplotype following spermatogonial transplantation. , 1994, Proceedings of the National Academy of Sciences of the United States of America.

[26]  M. Naito,et al.  Production of germline chimeric chickens, with high transmission rate of donor‐derived gametes, produced by transfer of primordial germ cells , 1994, Molecular reproduction and development.

[27]  R. Jones,et al.  Spermatogenesis in birds. , 1993, Oxford reviews of reproductive biology.

[28]  H. Murakami,et al.  Growth and utilization of nutrients in newly-hatched chick with or without removal of residual yolk. , 1992, Growth, development, and aging : GDA.

[29]  D. Froman,et al.  An improved procedure for intramagnal insemination of the chicken. , 1991, Poultry science.

[30]  B. Setchell,et al.  The testis and tissue transplantation: historical aspects. , 1990, Journal of reproductive immunology.

[31]  J. Smyth Genetics of plumage, skin and eye pigmentation in chickens. , 1990 .

[32]  P. Sørensen,et al.  Poultry breeding and genetics , 1990 .

[33]  B. Howarth Fertilizing ability of cock spermatozoa from the testis epididymis and vas deferens following intramagnal insemination. , 1983, Biology of reproduction.

[34]  W. Neaves,et al.  Transplantation of the testis. , 1979, Transplantation.