Efficiency of inactive vaccines against contagious agalactia in Brazil

This paper aims to evaluate the efficiency of three inactive vaccines against contagious agalactia prepared with samples of Mycoplasma agalactiae isolated in Brazil and different adjuvants. Vaccine 1 adsorbed with aluminum hydroxide was administered in 23 goats (Gc1) and 13 sheep (Gov1); vaccine 2 containing Montanide IMS-2215-VG was administered in 22 goats (Gc2) and 12 sheep (Gov2) and vaccine 3, containing Montanide Gel-01 was administered in 22 goats (Gc3) and 12 sheep (Gov3). All animals were negative for Ma at indirect ELISA and received two doses of 2mL each, subcutaneously, within a 21 day interval. Five animals from each species were used as control. Seventy-five days after the booster, four animals from each vaccinated group and two from the control group were challenged with 5mL of Ma culture containing 107cfu/mL, orally and through immersion of the female's udder in lactation. The serological response was analyzed during vaccination days (0 and 21) and at 51, 81, 111, 141 and 171 days after vaccination. The collection and analysis of the challenged animals were conducted at the day of the challenge (D0) and 7, 14, 21, 28, 35, 42, 49 and 56 days after the challenge. The three vaccines induced the production of antibodies, having no significant statistical difference (p<0.05). Animals from groups Gc1, Gc2 and Gov2 developed higher levels of antibodies, with significant statistical difference compared to the other vaccinated group and control group (p<0.05). After the challenge, the animals from the control presented an increase in regional lymph nodes and conjunctivitis, mastitis and arthritis. In four vaccinated animals, discrete conjunctivitis and congestion of the episcleral veins was observed. It is concluded that vaccines 1 and 2 induced levels of protective antibodies in goats and sheep, sufficient for clinical protection of the animals submitted to the experimental infection, indicating its use on the prevention of contagious agalactia.

[1]  E. O. Azevedo,et al.  Resposta imunológica em caprinos vacinados contra agalaxia contagiosa , 2013 .

[2]  Roberto Soares de Castro,et al.  Produção e avaliação de vacina contra agalaxia contagiosa , 2011 .

[3]  A. Fasanella,et al.  Long-term immunogenicity and protection against Mycoplasma agalactiae induced by an oil adjuvant vaccine in sheep. , 2010, Research in veterinary science.

[4]  E. O. Azevedo,et al.  ELISA protein G for the diagnosis of contagious agalactia in small ruminants , 2009 .

[5]  C. de la Fe,et al.  Correlating the immune response with the clinical-pathological course of persistent mastitis experimentally induced by Mycoplasma agalactiae in dairy goats. , 2009, Research in veterinary science.

[6]  V. Martella,et al.  An oil-emulsion vaccine induces full-protection against Mycoplasma agalactiae infection in sheep. , 2008, The new microbiologica.

[7]  P. Assunção,et al.  Field trial of a combined vaccine against caprine contagious agalactia: humoral immune response in lactating goats. , 2007, Veterinary journal.

[8]  E. O. Azevedo,et al.  Contagious agalactia by Mycoplasma agalactiae in small ruminants in Brazil: first report , 2006 .

[9]  G. Jula,et al.  Determination of antibody response against inactivated agalactia vaccine in small ruminants , 2005 .

[10]  A. Fasanella,et al.  Inactivated vaccine induces protection against Mycoplasma agalactiae infection in sheep. , 2002, The new microbiologica.

[11]  G. Leori,et al.  Experimental vaccination against Mycoplasma agalactiae using different inactivated vaccines. , 1999, Vaccine.

[12]  A. Fasanella,et al.  Persistence of antibodies to Mycoplasma agalactiae in vaccinated sheep. , 1998, The new microbiologica.

[13]  A. Perales,et al.  Immunoprophylaxis of caprine contagious agalactia due to Mycoplasma agalactiae with an inactivated vaccine , 1995, Veterinary Record.

[14]  S. A. Hasso,et al.  Antibody response patterns in goats experimentally infected with Mycoplasma agalactiae , 1994 .

[15]  A. Foggie,et al.  Contagious agalactia of sheep and goats. Immunity of lactating ewes vaccinated before mating with live or dead vaccines. , 1971, Journal of comparative pathology.

[16]  A. Foggie,et al.  Contagious agalactia of sheep and goats: preliminary studies on vaccines. , 1970, Journal of comparative pathology.