Cloning and Characterization of New Repetitive Sequences in Field Bean ( Vicia faba L.)

Five new repetitive sequences have been isolated from theVicia fabagenome, by cloning bands visible on agarose gel electrophoresis after digestion of genomic DNA with various restriction enzymes. The sequences were 109 to 584 bp long, their abundance ranging from 5×104to 5×105copies per haploid genome. Southern blot andin situhybridization revealed that four of five newly isolated repeats were dispersed in theV. fabagenome. One of the repeats (TIII15) showed tandem organization with several major hybridization spots on mitotic chromosomesin situ.These sites were distributed in euchromatic as well as in heterochromatic chromosomal regions, and in several loci they were simultaneously localized with previously describedFokI repeated elements. The sequence of TIII15 comprises four 26–27 bp subrepeats, but shares no homology toFokI elements which have similar sequence organization. All newly described sequences were highly specific forV. faba,with little or no hybridization to DNA of otherViciaspecies, and no hybridization to DNA of other legumes tested.

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