Toxinotyping of Clostridium perfringens Fecal Isolates of Reintroduced Père David's Deer (Elaphurus davidianus) in China

Abstract Clostridium perfringens is an important pathogen causing sudden death syndrome, necrotic enteritis, and gas gangrene in ruminants, especially some deer species. Père David's deer (Elaphurus davidianus) is one of the world's rare species and is an endangered and protected species in China. Some Père David's deer in the Chinese Shishou Père David's Deer Preserve died due to C. perfringens infection. We investigated the toxin types and C. perfringens enterotoxin-positive (cpe+) strains of isolated C. perfringens in Père David's deer in China. We collected 155 fecal samples from the Beijing Nanhaizi Père David's Deer Park and the Jiangsu Dafeng Père David's Deer National Nature Reserve between July 2010 and July 2011. Bacteria isolated using blood agar and mannitol agar plates were identified by Gram staining and nested PCR for 16S rRNA. We isolated C. perfringens from 41 fecal samples and used PCR amplification of five toxin genes to identify the toxinotypes and the cpe+ strains of C. perfringens. Twenty-one isolates were type A, 15 were type E, and five were type D. Fifteen isolates were cpe+ strains, including eight that were type A and seven that were type E.

[1]  B. McClane,et al.  Clostridium Perfringens Toxins Involved in Mammalian Veterinary Diseases , 2013, The open toxinology journal.

[2]  A. Al-Majali,et al.  Molecular typing and antimicrobial susceptibility of Clostridium perfringens from broiler chickens. , 2010, Anaerobe.

[3]  B. McClane,et al.  Clostridium Perfringens Toxins Involved in Mammalian Veterinary Diseases~!2009-08-24~!2009-09-11~!2010-03-09~! , 2010 .

[4]  R. Sting.  Detection of beta2 and major toxin genes by PCR in Clostridium perfringens field isolates of domestic animals suffering from enteritis or enterotoxaemia. , 2009, Berliner und Munchener tierarztliche Wochenschrift.

[5]  B. McClane,et al.  Prevalence of Enterotoxigenic Clostridium perfringens Isolates in Pittsburgh (Pennsylvania) Area Soils and Home Kitchens , 2007, Applied and Environmental Microbiology.

[6]  K. Miyamoto,et al.  Comparison of Virulence Plasmids among Clostridium perfringens Type E Isolates , 2007, Infection and Immunity.

[7]  B. McClane,et al.  Clostridium perfringens Enterotoxin Damages the Human Intestine In Vitro , 2005, Infection and Immunity.

[8]  C. Embury-Hyatt,et al.  Investigation of a syndrome of sudden death, splenomegaly, and small intestinal hemorrhage in farmed deer. , 2005, The Canadian veterinary journal = La revue veterinaire canadienne.

[9]  A. Hattel,et al.  A Retrospective Study of Mortality in Pennsylvania Captive White-Tailed Deer (Odocoileus Virginianus): 2000–2003 , 2004, Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc.

[10]  B. McClane,et al.  New insights into the cytotoxic mechanisms of Clostridium perfringens enterotoxin. , 2004, Anaerobe.

[11]  N. Kemper,et al.  Bacteria of Pathogenic Importance in Faeces from Cadavers of Free-ranging or Corralled Semi-domesticated Reindeer in Northern Norway , 2003, Veterinary Research Communications.

[12]  J. G. Smedley,et al.  The enteric toxins of Clostridium perfringens. , 2004, Reviews of physiology, biochemistry and pharmacology.

[13]  W. Sipos,et al.  Genotyping of Clostridium perfringens isolated from domestic and exotic ruminants and swine. , 2003, Journal of veterinary medicine. B, Infectious diseases and veterinary public health.

[14]  P. Valentin-Weigand,et al.  Toxin types of Clostridium perfringens isolated from free-ranging, semidomesticated reindeer in Norway , 2002, Veterinary Record.

[15]  E. Augustynowicz,et al.  Detection of enterotoxigenic Clostridium perfringens with a duplex PCR. , 2002, Journal of medical microbiology.

[16]  S. J. Billington,et al.  Clostridium perfringens Type E Animal Enteritis Isolates with Highly Conserved, Silent Enterotoxin Gene Sequences , 1998, Infection and Immunity.

[17]  D. L. Harris,et al.  Multiplex PCR assay for detection of Clostridium perfringens in feces and intestinal contents of pigs and in swine feed. , 1998, Veterinary microbiology.

[18]  Y. Sato,et al.  Gastric mucormycosis in a sika deer (Cervus nippon) associated with proliferation of Clostridium perfringens. , 1998, The Journal of veterinary medical science.

[19]  R. Carman.  Clostridium perfringens in spontaneous and antibiotic-associated diarrhoea of man and other animals , 1997 .

[20]  J. Songer.  Clostridial enteric diseases of domestic animals , 1996, Clinical microbiology reviews.

[21]  B. McClane,et al.  A conjugated synthetic peptide corresponding to the C-terminal region of Clostridium perfringens type A enterotoxin elicits an enterotoxin-neutralizing antibody response in mice , 1992, Infection and immunity.

[22]  R. J. Gilbert,et al.  Synthetic DNA probes for detection of enterotoxigenic Clostridium perfringens strains isolated from outbreaks of food poisoning , 1990, Journal of clinical microbiology.

[23]  A. English.  Enterotoxaemia caused by Clostridium perfringens type D in farmed fallow deer. , 1985, Australian veterinary journal.

[24]  K. Kummeneje,et al.  Clostridium perfringens enterotoxaemia in reindeer. , 1973, Nordisk veterinaermedicin.