Behavioural and endocrinological responses of mature male goldfish to the sex pheromone 17alpha,20beta-dihydroxy-4-pregnen-3-one in the water

The behavioural response of spermiated male goldfish to the sex pheromone 17alpha,20ss-dihydroxy-4-pregnen-3-one (17alpha,20ss-P) in the ambient water was measured using a computerized video-image analysis system. The position and spontaneous locomotor activity of single spermiated male goldfish were continuously recorded in an artificial stream. 17alpha,20ss-P (final concentration 10(-11) mol l-1) was supplied to one half and its ethanol carrier to the other half of the test area. The results showed that the fish spent significantly less time in water scented with 17alpha,20ss-P than in control water. Moreover, both the spontaneous locomotor activity and the gonadotropin II concentration in the plasma increased significantly because of contact with 17alpha,20ss-P in the ambient water. The swimming speed was unchanged, whether the fish resided in the 17alpha,20ss-P section or in the section without 17alpha,20ss-P added. In view of the complex dual hormone-pheromone system in goldfish, the possibility of an additional function of 17alpha,20ss-P is discussed. Because the release of the pheromone 17alpha,20ss-P occurs before ovulation and the level then drops drastically, we suggest that the avoidance reaction observed allows the sexually mature male to avoid misdirected courting of non-ovulated females and thus to continue its search for an ovulated female.

[1]  Wenbin Zheng,et al.  Endocrine, Gonadal and Behavioral Responses of Male Crucian Carp to the Hormonal Pheromone 17α,20β-dihydroxy-4-pregnen-3-one , 1995 .

[2]  R. Bjerselius,et al.  A study of the olfactory sensitivity of crucian carp (Carassius carassius) and goldfish (Carassius auratus) to 17α, 20β-dihydroxy-4-pregnen-3-one and prostaglandin F2α , 1993 .

[3]  P. Sorensen,et al.  Exposure to the pheromone 17α,20β-dihydroxy-4-pregnen-3-one enhances the behavioural spawning success, sperm production and sperm motility of male goldfish , 1993, Animal Behaviour.

[4]  B. M. Spruijt,et al.  SPONTANEOUS LOCOMOTOR ACTIVITY IN ARCTIC CHARR MEASURED BY A COMPUTERIZED IMAGING TECHNIQUE: ROLE OF BRAIN SEROTONERGIC ACTIVITY , 1993 .

[5]  Berry M. Spruijt,et al.  Approach, avoidance, and contact behavior of individually recognized animals automatically quantified with an imaging technique , 1992, Physiology & Behavior.

[6]  H. Kawauchi,et al.  Properties of common carp gonadotropin I and gonadotropin II. , 1992, General and comparative endocrinology.

[7]  F. Yamazaki.  The role of urine in sex discrimination in the goldfish Carassius auratus , 1990 .

[8]  R. Hurk,et al.  The chemical nature of sex attracting pheromones from the seminal vesicle of the African catfish, Clarias gariepinus , 1989 .

[9]  P. Sorensen,et al.  Differing behavioral and endocrinological effects of two female sex pheromones on male goldfish , 1989, Hormones and Behavior.

[10]  P. Sorensen,et al.  Direct evidence that 17α,20β-dihydroxy-4-pregnen-3-one functions as a goldfish primer pheromone: Preovulatory release is closely associated with male endocrine responses , 1989 .

[11]  P. Sorensen,et al.  F prostaglandins function as potent olfactory stimulants that comprise the postovulatory female sex pheromone in goldfish. , 1988, Biology of reproduction.

[12]  R. van den Hurk,et al.  The biosynthesis of steroid glucuronides in the testis of the zebrafish, Brachydanio rerio, and their pheromonal function as ovulation inducers. , 1987, General and comparative endocrinology.

[13]  K. Aida,et al.  Hormone changes during ovulation and effects of steroid hormones on plasma gonadotropin levels and ovulation in goldfish. , 1987, General and comparative endocrinology.

[14]  E. A. Huisman,et al.  The seminal vesicle as source of sex attracting substances in the African catfish, Clarias gariepinus , 1987 .

[15]  P. Sorensen,et al.  17α,20β-Dihydroxy-4-pregnen-3-one: a steroidal primer pheromone increasing milt volume in the goldfish, Carassius auratus , 1986 .

[16]  K. Olsén.  Chemo-attraction between juveniles of two sympatric stocks of Arctic charr, Salvelinus alpinus (L.), and their gene frequency of serum esterases , 1986 .

[17]  K. Olsén,et al.  Reduction by a surfactant of olfactory mediated attraction between juveniles of arctic charr, Salvelinus alpinus (L.) , 1985 .

[18]  R. Peter,et al.  Gonadotropin release from the pars distalis of goldfish, Carassius auratus, transplanted beside the brain or into the brain ventricles: additional evidence for gonadotropin-release-inhibitory factor. , 1984, General and comparative endocrinology.

[19]  R. Hurk,et al.  Ovarian steroid glucuronides function as sex pheromones for male zebrafish, Brachydanio rerio , 1983 .

[20]  N. Stacey,et al.  The role of pheromones in the sexual behaviour of the goldfish , 1976, Animal Behaviour.

[21]  C. Hemmings,et al.  OLFACTION AND VISION IN FISH SCHOOLING , 1966 .

[22]  K. Olsén,et al.  The significance of olfaction and social cues in milt availability, sexual hormone status, and spawning behavior of male rainbow trout (Oncorhynchus mykiss). , 1993, General and comparative endocrinology.

[23]  P. Sorensen,et al.  Electrophysiological Measures of Olfactory Sensitivity Suggest That Goldfish and Other Fish Use Species-Specific Mixtures of Hormones and Their Metabolites as Pheromones , 1992 .

[24]  P. Sorensen.  Hormones, pheromones and chemoreception , 1992 .

[25]  I. Fujita,et al.  The olfactory system, not the terminal nerve, functions as the primary chemosensory pathway mediating responses to sex pheromones in male goldfish. , 1991, Brain, behavior and evolution.

[26]  P. Sorensen,et al.  Function and evolution of fish hormonal pheromones , 1991 .

[27]  J. Blanc,et al.  Intraspecific chemocommunication in immature goldfish, Carassius auratus L.: attraction in olfactometer to free amino acid fractions from skin extracts , 1989 .

[28]  P. Sorensen,et al.  A steroid sex pheromone synchronizes male–female spawning readiness in goldfish , 1987, Nature.

[29]  T. Hara.  Role of Olfaction in Fish Behaviour , 1986 .

[30]  N. R. Liley,et al.  Chemical Communication in Fish , 1982 .