论文信息 - DEMOCHEM: Integrated system for mycotoxins detection

DEMOCHEM: Integrated system for mycotoxins detection

Abstract In this paper, we present an integrated system to detect Ochratoxin A (OTA) in feed, food and beverage. Its characteristics of portability, simplicity, sensitivity, low cost and user friendly, make the system suitable to be used by unskilled personnel. The system is based on a commercial Thin Layer Chromatography (TLC) plate coupled with an array of amorphous silicon (a-Si:H) photosensors embedded in a PTFE chamber where the chromatographic analysis occurs. The characterization of the system demonstrates its ability to detect OTA contained in wheat, wine and beer with a minimum detectable quantity of 0.2ng and a standard deviation below 5%.

[1] D Caputo,et al. Smart thin layer chromatography plate. , 2007, Lab on a chip.

[2] Dawei Deng,et al. Surface plasmon resonance biosensor for the detection of ochratoxin A in cereals and beverages. , 2009, Analytica chimica acta.

[3] Michele Suman,et al. Fluorescence Polarization Immunoassay for Rapid, Accurate and Sensitive Determination of Ochratoxin A in Wheat , 2014, Food Analytical Methods.

[4] Ying Wang,et al. Determination of Ochratoxin A in Cereals and Feeds by Ultra-performance Liquid Chromatography Coupled to Tandem Mass Spectrometry with Immunoaffinity Column Clean-up , 2014, Food Analytical Methods.

[5] J. Miller,et al. Analysis of wheat extracts for ochratoxin A by molecularly imprinted solid-phase extraction and pulsed elution , 2004, Analytical and bioanalytical chemistry.

[6] Domenico Caputo,et al. Linear photosensor array for on-chip food quality control based on thin layer chromatography , 2010 .

[7] Domenico Caputo,et al. Spectral tuned amorphous silicon p-i-n for DNA detection , 2006 .

[8] A. Nascetti,et al. Amorphous Silicon Photosensors for Detection of Ochratoxin a in Wine , 2012, IEEE Sensors Journal.