CARK3-mediated ADF4 regulates hypocotyl elongation and soil drought stress in Arabidopsis

Actin depolymerization factors (ADFs), as actin-binding proteins, act a crucial role in plant development and growth, as well as in response to abiotic and biotic stresses. Here, we found that CARK3 plays a role in regulating hypocotyl development and links a cross-talk between actin filament and drought stress through interaction with ADF4. By using bimolecular fluorescence complementation (BiFC) and GST pull-down, we confirmed that CARK3 interacts with ADF4 in vivo and in vitro. Next, we generated and characterized double mutant adf4cark3-4 and OE-ADF4:cark3-4. The hypocotyl elongation assay indicated that the cark3-4 mutant seedlings were slightly longer hypocotyls when compared with the wild type plants (WT), while CARK3 overexpressing seedlings had no difference with WT. In addition, overexpression of ADF4 significantly inhibited long hypocotyls of cark3-4 mutants. Surprisingly, we found that overexpression of ADF4 markedly enhance drought resistance in soil when compared with WT. On the other hand, drought tolerance analysis showed that overexpression of CARK3 could rescue adf4 drought susceptibility. Taken together, our results suggest that CARK3 acts as a regulator in hypocotyl elongation and drought tolerance likely via regulating ADF4 phosphorylation.

[1]  Yi Wang,et al.  Phosphorylation of the plasma membrane H+-ATPase AHA2 by BAK1 is required for ABA-induced stomatal closure in Arabidopsis. , 2022, The Plant cell.

[2]  Jianmei Wang,et al.  Monomerization of abscisic acid receptors through CARKs-mediated phosphorylation. , 2022, The New phytologist.

[3]  Qian Zhang,et al.  Binding of 14-3-3κ to ADF4 is involved in the regulation of hypocotyl growth and response to osmotic stress in Arabidopsis. , 2022, Plant science : an international journal of experimental plant biology.

[4]  Shanjin Huang,et al.  Functional Nonequivalence of Pollen ADF Isovariants in Arabidopsis. , 2022, The Plant journal : for cell and molecular biology.

[5]  Yan Guo,et al.  The PYR-PP2C-CKL2 module regulates ABA-mediated actin reorganization during stomatal closure. , 2021, The New phytologist.

[6]  Hyoungseok Lee,et al.  Abiotic Stress-Induced Actin-Depolymerizing Factor 3 From Deschampsia antarctica Enhanced Cold Tolerance When Constitutively Expressed in Rice , 2021, Frontiers in Physiology.

[7]  Yunjian He,et al.  Arabidopsis ADF1 Regulated by MYB73 is Involved in Response to Salt Stress via Affecting Actin Filaments Organization. , 2021, Plant & cell physiology.

[8]  L. Strader,et al.  Abscisic acid modulates auxin-responsive hypocotyl elongation , 2021, bioRxiv.

[9]  Yi Zhang,et al.  Profilin promotes formin-mediated actin filament assembly and vesicle transport during polarity formation in pollen. , 2021, The Plant cell.

[10]  Dongdong Ge,et al.  GhVLN4 is involved in multiple stress responses and required for resistance to Verticillium wilt. , 2021, Plant science : an international journal of experimental plant biology.

[11]  S. He,et al.  Arabidopsis calcium-dependent protein kinase 3 regulates actin cytoskeleton organization and immunity , 2020, Nature Communications.

[12]  Huize Chen,et al.  Actin filaments mediated root growth inhibition by changing their distribution under UV-B and hydrogen peroxide exposure in Arabidopsis , 2020, Biological Research.

[13]  Hsi-Hua Wang,et al.  Close arrangement of CARK3 and PMEIL affects ABA-mediated pollen sterility in Arabidopsis thaliana. , 2020, Plant, cell & environment.

[14]  W. Gray,et al.  Rapid Auxin-Mediated Cell Expansion. , 2020, Annual review of plant biology.

[15]  Li Xu,et al.  Gibberellins modulate shade-induced soybean hypocotyl elongation downstream of the mutual promotion of auxin and brassinosteroids. , 2020, Plant physiology and biochemistry : PPB.

[16]  Rongcheng Lin,et al.  PHYTOCHROME-INTERACTING FACTORS interact with the ABA receptors PYL8 and PYL9 to orchestrate ABA signaling in darkness. , 2020, Molecular plant.

[17]  R. S. Arieti,et al.  Auxin‐induced actin cytoskeleton rearrangements require AUX1 , 2019, The New phytologist.

[18]  Wei Wang,et al.  Wheat F-box Protein TaFBA1 Positively Regulates Plant Drought Tolerance but Negatively Regulates Stomatal Closure , 2019, Front. Plant Sci..

[19]  Z. Nikoloski,et al.  The Rice Actin-Binding Protein RMD Regulates Light-Dependent Shoot Gravitropism1[OPEN] , 2019, Plant Physiology.

[20]  Shanjin Huang,et al.  Mechanism of CAP1-mediated apical actin polymerization in pollen tubes , 2019, Proceedings of the National Academy of Sciences.

[21]  K. Jiang,et al.  Arabidopsis ADF5 promotes stomatal closure by regulating actin cytoskeleton remodeling in response to ABA and drought stress , 2018, Journal of experimental botany.

[22]  Yi Zhang,et al.  Actin Polymerization Mediated by AtFH5 Directs the Polarity Establishment and Vesicle Trafficking for Pollen Germination in Arabidopsis. , 2018, Molecular plant.

[23]  Yuna Sun,et al.  CARK1 mediates ABA signaling by phosphorylation of ABA receptors , 2018, Cell Discovery.

[24]  Shanjin Huang,et al.  ADF10 shapes the overall organization of apical actin filaments by promoting their turnover and ordering in pollen tubes , 2017, Journal of Cell Science.

[25]  Yanxiu Zhao,et al.  CASEIN KINASE1-LIKE PROTEIN2 Regulates Actin Filament Stability and Stomatal Closure via Phosphorylation of Actin Depolymerizing Factor , 2016, Plant Cell.

[26]  Z. Kang,et al.  TaADF3, an Actin-Depolymerizing Factor, Negatively Modulates Wheat Resistance Against Puccinia striiformis , 2016, Front. Plant Sci..

[27]  Yanxiu Zhao,et al.  14-3-3 λ protein interacts with ADF1 to regulate actin cytoskeleton dynamics in Arabidopsis , 2015, Science China Life Sciences.

[28]  Qun Zhang,et al.  Phospholipids: molecules regulating cytoskeletal organization in plant abiotic stress tolerance. , 2014, Plant signaling & behavior.

[29]  Shanjin Huang,et al.  Arabidopsis ACTIN-DEPOLYMERIZING FACTOR7 Severs Actin Filaments and Regulates Actin Cable Turnover to Promote Normal Pollen Tube Growth[W] , 2013, Plant Cell.

[30]  Fiona C. Denison,et al.  14-3-3 phosphoprotein interaction networks – does isoform diversity present functional interaction specification? , 2012, Front. Plant Sci..

[31]  L. An,et al.  Overexpression of profilin 3 affects cell elongation and F-actin organization in Arabidopsis thaliana , 2012, Plant Cell Reports.

[32]  L. Blanchoin,et al.  Arabidopsis Actin Depolymerizing Factor4 Modulates the Stochastic Dynamic Behavior of Actin Filaments in the Cortical Array of Epidermal Cells[C][W] , 2011, Plant Cell.

[33]  P. Hussey,et al.  Plant Actin Biology , 2009 .

[34]  P. Hussey,et al.  The Actin-Interacting Protein AIP1 Is Essential for Actin Organization and Plant Development , 2004, Current Biology.

[35]  Y. Lee,et al.  Abscisic acid-induced actin reorganization in guard cells of dayflower is mediated by cytosolic calcium levels and by protein kinase and protein phosphatase activities. , 2001, Plant physiology.