Decreased hydrogen production leads to selective butanol production in co-cultures of Clostridium thermocellum and Clostridium saccharoperbutylacetonicum strain N1-4.

When Clostridium thermocellum and Clostridium saccharoperbutylacetonicum strain N1-4 were co-cultured hydrogen production decreased and butanol was selectively produced with extremely low level of acetone. Since the high butanol production correlates with low hydrogen production, the molecular selection of hydrogenase gene activity is expected to yield strains exhibiting a higher butanol ratio.

[1]  Arnold L. Demain,et al.  Biosolutions to the energy problem , 2009, Journal of Industrial Microbiology & Biotechnology.

[2]  L. Yerushalmi,et al.  Effect of increased hydrogen partial pressure on the acetone-butanol fermentation by Clostridium acetobutylicum , 1985, Applied Microbiology and Biotechnology.

[3]  Yanping Zhang,et al.  Controlling the oxidoreduction potential of the culture of Clostridium acetobutylicum leads to an earlier initiation of solventogenesis, thus increasing solvent productivity , 2011, Applied Microbiology and Biotechnology.

[4]  H. Bahl,et al.  Modifying the product pattern of Clostridium acetobutylicum , 2012, Applied Microbiology and Biotechnology.

[5]  D. T. Jones,et al.  Acetone-butanol fermentation revisited. , 1986, Microbiological reviews.

[6]  Yanning Zheng,et al.  Problems with the microbial production of butanol , 2009, Journal of Industrial Microbiology & Biotechnology.

[7]  P. Soucaille,et al.  Regulation of carbon and electron flow in Clostridium acetobutylicum grown in chemostat culture at neutral pH on mixtures of glucose and glycerol , 1994, Journal of bacteriology.

[8]  K. Furukawa,et al.  Metabolic engineering for solvent productivity by downregulation of the hydrogenase gene cluster hupCBA in Clostridium saccharoperbutylacetonicum strain N1-4 , 2008, Applied Microbiology and Biotechnology.

[9]  Atsumi Nakazato,et al.  Butanol Production from Crystalline Cellulose by Cocultured Clostridium thermocellum and Clostridium saccharoperbutylacetonicum N1-4 , 2011, Applied and Environmental Microbiology.

[10]  P. Dürre.  Biobutanol: An attractive biofuel , 2007, Biotechnology journal.

[11]  Weihong Jiang,et al.  Disruption of the acetoacetate decarboxylase gene in solvent-producing Clostridium acetobutylicum increases the butanol ratio. , 2009, Metabolic engineering.

[12]  Yukihiro Tashiro,et al.  Novel high-efficient butanol production from butyrate by non-growing Clostridium saccharoperbutylacetonicum N1-4 (ATCC 13564) with methyl viologen. , 2007, Journal of bioscience and bioengineering.