Deoxynivalenol (DON) is a common food borne mycotoxin and occurs predominantly in grains such as wheat, barley, oats, etc. DON induces systemic health problems such as loss of appetite, emesis and diarrhea in both human and farm animals. Reliable diagnostic parameters for DON intoxication are needed to prevent deep health impact. In order to establish useful diagnostic parameters, we investigated clinical signs, hematological values, serum biochemical values, gross-, histo- and toxico-pathological findings in B6C3F1 male mice after oral administration of DON (0.83, 2.5 and 7.5 mg/kg) for 8 days. Body weight gain was significantly decreased at the highest dose of DON. Anorexia, ataxia, for crudness and lack of vigor were observed at the highest dose DON group. In hematological values, the numbers of WBC and platelets and hemoglobin content were reduced with decreased neutrophil and monocytes by 7.5 mg/kg DON. Prothrombin time (PT) and activated partial thromboplastin time (aPTT) were prolonged in a dose-dependent manner and the content of fibrinogen was elevated at high dose of DON. Of serum biochemical values, total protein, globulin, BUN, cholesterol and test-osterone were reduced but total bilirubin and albumin/globulin ratio increased. The enzyme activity of alkaline phosphatase was decreased while that of alanine aminotransferase was elevated. Relative organ weights of thymus, seminal vesicle/prostate and testes were dose-dependently reduced but those of liver and left adrenal gland increased with dose dependency. As for pathological findings, atrophy of thymus, seminal vesicle/prostate and testes and submucosal edema and ulceration in stomach and depletion of lymphocytes in thymus cortex were observed. In conclusion, these clinical, hematological, blood biochemical and patholgical parameters obtained in the present studies can be used for diagnosis of DON-mycotoxicosis, especially, low WBC, platelets, protein, BUN and testosterone and delayed prothrombin time can be available as for reliable diagnostic parameters.
[1]
S. Dänicke,et al.
Effects of the Fusarium toxin deoxynivalenol from naturally contaminated wheat given subchronically or as one single dose on the in vivo protein synthesis of peripheral blood lymphocytes and plasma proteins in the pig.
,
2006,
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association.
[2]
J. Corton,et al.
GENE EXPRESSION PROFILING IN SPLEENS OF DEOXYNIVALENOL-EXPOSED MICE: IMMEDIATE EARLY GENES AS PRIMARY TARGETS
,
2004,
Journal of toxicology and environmental health. Part A.
[3]
C. Wild,et al.
Development of a urinary biomarker of human exposure to deoxynivalenol.
,
2003,
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association.
[4]
E. Creppy.
Update of survey, regulation and toxic effects of mycotoxins in Europe.
,
2002,
Toxicology letters.
[5]
J. Pestka,et al.
Effects of 8-week exposure of the B6C3F1 mouse to dietary deoxynivalenol (vomitoxin) and zearalenone.
,
1986,
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association.
[6]
V. Betina.
Structure-activity relationships among mycotoxins.
,
1989,
Chemico-biological interactions.
[7]
W. Thompson,et al.
Structure-function relationships of 12,13-epoxytrichothecene mycotoxins in cell culture: comparison to whole animal lethality.
,
1986,
Toxicon : official journal of the International Society on Toxinology.