The objective of this work was to characterize the fat globule and milk fat globule membrane (MFGM) proteins in Maiwa (n = 6), Gannan (n = 4) and Zhongdian yak whole milks (n = 4). Fat globule size distribution and specific fat surface area of whole yak milk were determined using a laser diffraction particle size analyzer. The average specific fat surface area of Gannan yak milk (2.05 m2·mg−1) and Zhongdian yak milk (2.01 m2·mg−1) was higher (P < 0.05) than that of Maiwa yak milk (1.92 m2·mg−1). The total protein content of MFGM and surface protein coverage of fat globule in Maiwa yak milk (11.12 mg·g−1 fat and 5.67 mg·m−2, respectively) and Gannan yak milk (12.24 mg·g−1 fat and 5.97 mg·m−2, respectively) were higher (P < 0.05) than that of Zhongdian yak milk (9.72 mg·g−1 fat and 4.81 mg·m−2, respectively). Protein components in the MFGM of yak milk were analyzed using an Ultrascan XL laser densitometer and an LKB 2400 GelScanXL software program. No major differences (P > 0.05%) in the protein patterns on the SDS gel were observed among the MFGM of Maiwa yak, Gannan yak and Zhongdian yak, with the exception of mucin 1 and butyrophilin which were significantly lower (P < 0.05) in the MFGM of Zhongdian yak milk.Abstract(n = 6) (n = 4) (n = 6) (MFGM) (2.05 m2·mg−1) (2.01 m2·mg−1) (P < 0.05) (1.92 m2·mg−1) MFGM (11.12 mg·g−1) (5.67 mg·m−2) MFGM (12.24 mg·g−1) (5.97 mg·m−2) (P < 0.05) MFGM (9.72 mg·g−1) (4.81 mg·m−2) MFGM Ultrascan XL LKB 2400 GelScanXL MFGM (MUC1) (BUT) (P > 0.05), SDS-PAGE MFGM (P > 0.05)RésuméL’objectif de ce travail était de caractériser les globules gras et les protéines de membrane de globule gras du lait entier de yak de races Maiwa (n = 6), Gannan (n = 4) et Zhongdian (n = 4). La distribution de taille des globule gras et la surface spécifique du lait entier de yak ont été déterminées par mesure de diffraction laser. La surface spécifique moyenne des globules gras du lait de yak de race Gannan (2,05 m2·mg−1) et de race Zhongdian (2,01 m2·mg−1) était significativement plus élevée (P < 0,05) que celle obtenue pour le lait de yak de race Maiwa (1,92 m2·mg−1). La teneur en protéines totales de la membrane du globule gras du lait de yak et la couverture en protéine de surface étaient plus élevées (P < 0,05) dans le lait de yak de race Maiwa (respectivement 11,12 mg·g−1 de matière grasse et 5,67 mg·m−2) et du lait de yak de race Gannan (respectivement 12,24 mg·g−1 de matière grasse et 5,97 mg·m−2) par rapport au lait de yak de race Zhongdian (respectivement 9,72 mg·g−1 de matière grasse et 4,81 mg·m−2). Les composants protéiques de la membrane de globule gras du lait de yak ont été analysés par densitométrie laser Ultrascan XL à l’aide du logiciel GelScanXL LKB 2400. Aucune différence (P > 0,05) n’a été observée dans les profils protéiques des gels SDS obtenus à partir des membranes de globule gras du lait de yak des trois races, à l’exception d’un pourcentage significativement plus faible (P < 0,05) en mucine 1 et en butyrophiline pour le lait de yak de race Zhongdian.
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