Brine shrimp lethality bioassay of some Verbascum species growing in Turkey

Verbascum L. (Scrophulariaceae) species are medicinal plants that have been used for the treatment of inflammatory diseases, asthma, spasmodic coughs and other pulmonary problems in Anatolia and in several countries. The objective of this study was to assess the in vivo cytotoxic activities of thirteen Verbascum extracts including V. chionophyllum Hub.-Mor., V. cilicicum Boiss., V. dudleyanum (Hub.-Mor.) Hub.Mor., V. lasianthum Boiss., V. latisepalum Hub.-Mor., V. mucronatum Lam., V. olympicum Boiss., V. pterocalycinum var. mutense Hub.-Mor., V. pycnostachyum Boiss. & Heldr., V. salviifolium Boiss., V. splendidum Boiss., V. stachydifolium Boiss. & Heldr and V. uschackense (Murb.) Hub.-Mor. using brine shrimp (Artemia salina) lethality bioassay. The methanolic extracts of V. chionophyllum flowers and leaves, V. cilicicum flowers, V. lasianthum flowers, V. mucronatum flowers, V. pycnostachyum flowers and V. splendidum flowers showed the highest inhibitory activities against the brine shrimp. On the other hand, the rest of the species did not show any remarkable cytotoxic activity.

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