Single-molecule emulsion PCR in microfluidic droplets
暂无分享,去创建一个
Zhi Zhu | Chaoyong James Yang | Zhichao Guan | Zhi Zhu | C. Yang | G. Jenkins | Wenhua Zhang | Mingxia Zhang | Zhichao Guan | Gareth Jenkins | Mingxia Zhang | Wenhua Zhang
[1] Helen Song,et al. Reactions in droplets in microfluidic channels. , 2006, Angewandte Chemie.
[2] A. Manz,et al. Lab-on-a-chip: microfluidics in drug discovery , 2006, Nature Reviews Drug Discovery.
[3] Gerald F. Joyce,et al. Selection in vitro of an RNA enzyme that specifically cleaves single-stranded DNA , 1990, Nature.
[4] Chris R Kleijn,et al. Predictive model for the size of bubbles and droplets created in microfluidic T-junctions. , 2010, Lab on a chip.
[5] Viktor Stein,et al. Continuous-flow polymerase chain reaction of single-copy DNA in microfluidic microdroplets. , 2009, Analytical chemistry.
[6] Jürgen Popp,et al. Droplet formation via flow-through microdevices in Raman and surface enhanced Raman spectroscopy--concepts and applications. , 2011, Lab on a chip.
[7] B. M. Paegel. Microfluidic landscapes for evolution. , 2010, Current opinion in chemical biology.
[8] Helen Song,et al. A microfluidic system for controlling reaction networks in time. , 2003, Angewandte Chemie.
[9] A. Lee,et al. 1-Million droplet array with wide-field fluorescence imaging for digital PCR. , 2011, Lab on a chip.
[10] Yiqiong Zhao,et al. Compartmentalization of chemically separated components into droplets. , 2009, Angewandte Chemie.
[11] J. Lupski,et al. The complete genome of an individual by massively parallel DNA sequencing , 2008, Nature.
[12] Chaoyong James Yang,et al. Highly parallel single-molecule amplification approach based on agarose droplet polymerase chain reaction for efficient and cost-effective aptamer selection. , 2012, Analytical chemistry.
[13] Chaoyong James Yang,et al. Agarose droplet microfluidics for highly parallel and efficient single molecule emulsion PCR. , 2010, Lab on a chip.
[14] J. Shuga,et al. Single-cell multiplex gene detection and sequencing with microfluidically generated agarose emulsions. , 2011, Angewandte Chemie.
[15] H. Stone,et al. Formation of dispersions using “flow focusing” in microchannels , 2003 .
[16] A. Theberge,et al. Microdroplets in microfluidics: an evolving platform for discoveries in chemistry and biology. , 2010, Angewandte Chemie.
[17] T. G. Mitchell,et al. Multiplexed real-time polymerase chain reaction on a digital microfluidic platform. , 2010, Analytical chemistry.
[18] R. Garrell,et al. Droplet-based microfluidics with nonaqueous solvents and solutions. , 2006, Lab on a chip.
[19] A. Griffiths,et al. Reliable microfluidic on-chip incubation of droplets in delay-lines. , 2009, Lab on a chip.
[20] M. Reetz,et al. Superior Biocatalysts by Directed Evolution , 1999 .
[21] Phil Paik,et al. Electrowetting-based droplet mixers for microfluidic systems. , 2003, Lab on a chip.
[22] Magalie Faivre,et al. Microfluidic flow focusing: Drop size and scaling in pressure versus flow‐rate‐driven pumping , 2005, Electrophoresis.
[23] Amit Gupta,et al. Effect of geometry on droplet formation in the squeezing regime in a microfluidic T-junction , 2010 .
[24] Abraham P. Lee,et al. Microfluidic sorting of droplets by size , 2008 .
[25] C. Culbertson,et al. Chemical analysis of single mammalian cells with microfluidics. Strategies for culturing, sorting, trapping, and lysing cells and separating their contents on chips. , 2007, Analytical chemistry.
[26] Richard A Mathies,et al. Inline injection microdevice for attomole-scale sanger DNA sequencing. , 2007, Analytical chemistry.
[27] Arun Majumdar,et al. Mixing crowded biological solutions in milliseconds. , 2005, Analytical chemistry.
[28] M. Roth,et al. Digital reaction technology by micro segmented flow—components, concepts and applications , 2004 .
[29] Xiaohong Fang,et al. Aptamers generated from cell-SELEX for molecular medicine: a chemical biology approach. , 2010, Accounts of chemical research.
[30] Chaoyong James Yang,et al. High-throughput single copy DNA amplification and cell analysis in engineered nanoliter droplets. , 2008, Analytical chemistry.
[31] I. Mezić,et al. Chaotic Mixer for Microchannels , 2002, Science.
[32] A. Halpern,et al. A Sanger/pyrosequencing hybrid approach for the generation of high-quality draft assemblies of marine microbial genomes. , 2006, Proceedings of the National Academy of Sciences of the United States of America.
[33] J. Sage,et al. Cellular mechanisms of tumour suppression by the retinoblastoma gene , 2008, Nature Reviews Cancer.
[34] D. Weitz,et al. Fluorescence-activated droplet sorting (FADS): efficient microfluidic cell sorting based on enzymatic activity. , 2009, Lab on a chip.
[35] Kiwamu Saito,et al. Imaging of single fluorescent molecules and individual ATP turnovers by single myosin molecules in aqueous solution , 1995, Nature.
[36] D. Weitz,et al. Monodisperse Double Emulsions Generated from a Microcapillary Device , 2005, Science.
[37] D. Weitz,et al. Geometrically mediated breakup of drops in microfluidic devices. , 2003, Physical review letters.
[38] Wolf-Dieter Fessner,et al. Biocatalysis: From Discovery to Application , 2000 .
[39] Bill W Colston,et al. High-throughput quantitative polymerase chain reaction in picoliter droplets. , 2008, Analytical chemistry.
[40] Jeff Mellen,et al. High-Throughput Droplet Digital PCR System for Absolute Quantitation of DNA Copy Number , 2011, Analytical chemistry.
[41] Shinji Katsura,et al. Single-molecule PCR using water-in-oil emulsion. , 2003, Journal of biotechnology.
[42] S. Weiss. Fluorescence spectroscopy of single biomolecules. , 1999, Science.
[43] L. Mazutis,et al. Quantitative and sensitive detection of rare mutations using droplet-based microfluidics. , 2011, Lab on a chip.
[44] J. Raser,et al. Noise in Gene Expression: Origins, Consequences, and Control , 2005, Science.
[45] J. Silver,et al. Nanoliter scale PCR with TaqMan detection. , 1997, Nucleic acids research.
[46] S. Cho,et al. Creating, transporting, cutting, and merging liquid droplets by electrowetting-based actuation for digital microfluidic circuits , 2003 .
[47] Qun Zhong,et al. Multiplex digital PCR: breaking the one target per color barrier of quantitative PCR. , 2011, Lab on a chip.
[48] N. Nguyen,et al. An investigation on the mechanism of droplet formation in a microfluidic T-junction , 2011 .
[49] Yanwei Jia,et al. Simple, robust storage of drops and fluids in a microfluidic device. , 2009, Lab on a chip.
[50] Michele Zagnoni,et al. Hysteresis in multiphase microfluidics at a T-junction. , 2010, Langmuir : the ACS journal of surfaces and colloids.
[51] A. Delcher,et al. Human, mouse, and rat genome large-scale rearrangements: stability versus speciation. , 2004, Genome research.
[52] Shoji Takeuchi,et al. Utilization of cell-sized lipid containers for nanostructure and macromolecule handling in microfabricated devices. , 2005, Analytical chemistry.
[53] Victoria Wilson,et al. Repeat unit sequence variation in minisatellites: A novel source of DNA polymorphism for studying variation and mutation by single molecule analysis , 1990, Cell.
[54] S. Foote,et al. Colorimetric detection of specific DNA segments amplified by polymerase chain reactions. , 1989, Proceedings of the National Academy of Sciences of the United States of America.
[55] David A Weitz,et al. Controlling droplet incubation using close-packed plug flow. , 2011, Biomicrofluidics.
[56] Razvan Nutiu,et al. In vitro selection of structure-switching signaling aptamers. , 2005, Angewandte Chemie.
[57] Paul A Dayton,et al. On-chip generation of microbubbles as a practical technology for manufacturing contrast agents for ultrasonic imaging. , 2007, Lab on a chip.
[58] K. Mullis,et al. Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia. , 1985, Science.
[59] T. Misteli,et al. The emerging role of nuclear architecture in DNA repair and genome maintenance , 2009, Nature Reviews Molecular Cell Biology.
[60] Michelle D. Wang,et al. Force and velocity measured for single molecules of RNA polymerase. , 1998, Science.
[61] G. Whitesides,et al. Emulsification in a microfluidic flow-focusing device: effect of the viscosities of the liquids , 2008 .
[62] Levent Yobas,et al. High-performance flow-focusing geometry for spontaneous generation of monodispersed droplets. , 2006, Lab on a chip.
[63] Nancy L Allbritton,et al. CRITICAL REVIEW www.rsc.org/loc | Lab on a Chip Analysis of single mammalian cells on-chip , 2006 .
[64] D. Dressman,et al. Transforming single DNA molecules into fluorescent magnetic particles for detection and enumeration of genetic variations , 2003, Proceedings of the National Academy of Sciences of the United States of America.
[65] James R. Knight,et al. Genome sequencing in microfabricated high-density picolitre reactors , 2005, Nature.
[66] Feng Wang,et al. Emulsion PCR: A High Efficient Way of PCR Amplification of Random DNA Libraries in Aptamer Selection , 2011, PloS one.
[67] Dan Bratton,et al. Static microdroplet arrays: a microfluidic device for droplet trapping, incubation and release for enzymatic and cell-based assays. , 2009, Lab on a chip.
[68] Benjamin J Hindson,et al. On-chip, real-time, single-copy polymerase chain reaction in picoliter droplets. , 2007, Analytical chemistry.
[69] Andrew D Griffiths,et al. Droplet-based microfluidic systems for high-throughput single DNA molecule isothermal amplification and analysis. , 2009, Analytical chemistry.
[70] L. Gold,et al. Systematic evolution of ligands by exponential enrichment: RNA ligands to bacteriophage T4 DNA polymerase. , 1990, Science.
[71] K. Mullis,et al. Specific enzymatic amplification of DNA in vitro: the polymerase chain reaction. , 1986, Cold Spring Harbor symposia on quantitative biology.
[72] Henry A. Erlich,et al. Amplification and analysis of DNA sequences in single human sperm and diploid cells , 1988, Nature.
[73] M. Ronaghi,et al. A Sequencing Method Based on Real-Time Pyrophosphate , 1998, Science.
[74] S. Nasim,et al. Nested polymerase chain reaction assay for the detection of cytomegalovirus overcomes false positives caused by contamination with fragmented DNA , 1990, Journal of medical virology.
[75] Wei Wang,et al. On-demand microfluidic droplet trapping and fusion for on-chip static droplet assays. , 2009, Lab on a chip.
[76] Gene-Wei Li,et al. Central dogma at the single-molecule level in living cells , 2011, Nature.
[77] S. Takayama,et al. Gravity-driven microfluidic particle sorting device with hydrodynamic separation amplification. , 2007, Analytical chemistry.
[78] X. Xie,et al. Living Cells as Test Tubes , 2006, Science.
[79] Da Xing,et al. Single-molecule DNA amplification and analysis using microfluidics. , 2010, Chemical reviews.
[80] K K Kidd,et al. Haplotype of multiple polymorphisms resolved by enzymatic amplification of single DNA molecules. , 1990, Proceedings of the National Academy of Sciences of the United States of America.
[81] Wilhelm T S Huck,et al. Surface-induced droplet fusion in microfluidic devices. , 2007, Lab on a chip.
[82] S. Quake,et al. Dynamic pattern formation in a vesicle-generating microfluidic device. , 2001, Physical review letters.
[83] Frank Diehl,et al. BEAMing: single-molecule PCR on microparticles in water-in-oil emulsions , 2006, Nature Methods.
[84] Ya-Wen Sun,et al. Note on new massive gravity in AdS(3) , 2009, 0903.0536.
[85] Daniel T. Chiu,et al. Chemistry and biology in femtoliter and picoliter volume droplets. , 2009, Accounts of chemical research.
[86] S. D. Hudson,et al. Microfluidic approach for rapid multicomponent interfacial tensiometry. , 2006, Lab on a chip.
[87] Charles N Baroud,et al. Dynamics of microfluidic droplets. , 2010, Lab on a chip.
[88] Shoji Takeuchi,et al. Timing controllable electrofusion device for aqueous droplet-based microreactors. , 2006, Lab on a chip.
[89] Igor L. Medintz,et al. Single-molecule DNA amplification and analysis in an integrated microfluidic device. , 2001, Analytical chemistry.
[90] Shanavaz Nasarabadi,et al. On-chip single-copy real-time reverse-transcription PCR in isolated picoliter droplets. , 2007, Analytical chemistry.
[91] Joshua D. Tice,et al. Microfluidic systems for chemical kinetics that rely on chaotic mixing in droplets , 2004, Philosophical Transactions of the Royal Society of London. Series A: Mathematical, Physical and Engineering Sciences.
[92] A. Plückthun,et al. In vitro selection and evolution of proteins. , 2000, Advances in protein chemistry.
[93] E. Check. Human genome: Patchwork people , 2005, Nature.
[94] A. Jeffreys,et al. Amplification of human minisatellites by the polymerase chain reaction: towards DNA fingerprinting of single cells. , 1988, Nucleic acids research.
[95] Toshiro Higuchi,et al. Droplet formation in a microchannel network. , 2002, Lab on a chip.
[96] George M. Whitesides,et al. An Axisymmetric Flow‐Focusing Microfluidic Device , 2005 .
[97] Vittorio Cristini,et al. Design of microfluidic channel geometries for the control of droplet volume, chemical concentration, and sorting. , 2004, Lab on a chip.
[98] Richard Novak,et al. High-performance single cell genetic analysis using microfluidic emulsion generator arrays. , 2010, Analytical chemistry.
[99] M. Stratton,et al. The cancer genome , 2009, Nature.
[100] J. Szostak,et al. In vitro selection of RNA molecules that bind specific ligands , 1990, Nature.
[101] Monpichar Srisa-Art,et al. Microdroplets: a sea of applications? , 2008, Lab on a chip.
[102] J. Shendure,et al. Materials and Methods Som Text Figs. S1 and S2 Tables S1 to S4 References Accurate Multiplex Polony Sequencing of an Evolved Bacterial Genome , 2022 .
[103] Ximin He,et al. A double droplet trap system for studying mass transport across a droplet-droplet interface. , 2010, Lab on a chip.
[104] Teodor Veres,et al. Two-dimensional droplet-based surface plasmon resonance imaging using electrowetting-on-dielectric microfluidics. , 2009, Lab on a chip.
[105] Thomas Schneider,et al. Systematic investigation of droplet generation at T-junctions. , 2011, Lab on a chip.
[106] A. Lee,et al. Alternating droplet generation and controlled dynamic droplet fusion in microfluidic device for CdS nanoparticle synthesis. , 2006, Lab on a chip.
[107] Helene Andersson-Svahn,et al. Overview of single-cell analyses: microdevices and applications. , 2010, Lab on a chip.
[108] Jonathan E. Allen,et al. The Genome of the Basidiomycetous Yeast and Human Pathogen Cryptococcus neoformans , 2005, Science.
[109] R. Jaenicke,et al. Advances in protein chemistry, vol. 29 , 1976 .
[110] D. Weitz,et al. Electric control of droplets in microfluidic devices. , 2006, Angewandte Chemie.
[111] Wei Liu,et al. The effect of interfacial tension on droplet formation in flow-focusing microfluidic device , 2011, Biomedical microdevices.