Determination of collagen fiber orientation in human tissue by use of polarization measurement of molecular second-harmonic-generation light.

Based on the reflection-type polarization measurement of second-harmonic-generation (SHG) light induced by collagen molecules, we are able to determine the collagen fiber orientation in human tissues taken from a cadaver. The resulting SHG radar graph shows the direction of the absolute orientation and the degree of organization of collagen fibers. To evaluate the probing sensitivity to the collagen orientation, we compared the proposed method with other polarimetric methods. Use of the proposed method revealed characteristic orientation differences among collagen fibers and demonstrated significant inhomogeneity with respect to the distribution of collagen orientation in human dentin. The proposed method provides a powerful research and diagnostic tool for examining the collagen orientation in human tissues.

[1]  S Fine,et al.  Optical second harmonic generation in biological systems. , 1971, Applied optics.

[2]  D K Yue,et al.  Use of X-Ray Diffraction in Study of Human Diabetic and Aging Collagen , 1991, Diabetes.

[3]  Beop-Min Kim,et al.  Polarization-dependent optical second-harmonic imaging of a rat-tail tendon. , 2002, Journal of biomedical optics.

[4]  J M Schmitt,et al.  Subsurface imaging of living skin with optical coherence microscopy. , 1995, Dermatology.

[5]  S. Osaki,et al.  Distribution map of collagen fiber orientation in a whole calf skin , 1999, The Anatomical record.

[6]  W. R. Wiley,et al.  Three-Dimensional Vibrational Imaging by Coherent Anti-Stokes Raman Scattering , 1999 .

[7]  G. Ripandelli,et al.  Optical coherence tomography. , 1998, Seminars in ophthalmology.

[8]  Patrick Stoller,et al.  Polarization-modulated second harmonic generation in collagen. , 2002, Biophysical journal.

[9]  Kostas Politopoulos,et al.  Second and third optical harmonic generation in type I collagen, by nanosecond laser irradiation, over a broad spectral region , 2000 .

[10]  Takeshi Yasui,et al.  Characterization of collagen orientation in human dermis by two-dimensional second-harmonic-generation polarimetry. , 2004, Journal of biomedical optics.

[11]  B Eyden,et al.  Structural variations of collagen in normal and pathological tissues: role of electron microscopy. , 2001, Micron.

[12]  T. Araki,et al.  Applications of fluorescence microscopy to studies of dental hard tissue. , 2001, Frontiers of medical and biological engineering : the international journal of the Japan Society of Medical Electronics and Biological Engineering.

[13]  I. Freund,et al.  Optical second‐harmonic scattering in rat‐tail tendon , 1981, Biopolymers.

[14]  W. Webb,et al.  Live tissue intrinsic emission microscopy using multiphoton-excited native fluorescence and second harmonic generation , 2003, Proceedings of the National Academy of Sciences of the United States of America.

[15]  William A Mohler,et al.  Three-dimensional high-resolution second-harmonic generation imaging of endogenous structural proteins in biological tissues. , 2002, Biophysical journal.

[16]  J. Izatt,et al.  Real-time in vivo imaging of human gastrointestinal ultrastructure by use of endoscopic optical coherence tomography with a novel efficient interferometer design. , 1999, Optics letters.

[17]  J. Eichler,et al.  Frequency doubling of ultrashort laser pulses in biological tissues. , 1999, Applied optics.

[18]  Bruce J Tromberg,et al.  Selective corneal imaging using combined second-harmonic generation and two-photon excited fluorescence. , 2002, Optics letters.

[19]  L M Loew,et al.  Second-harmonic imaging microscopy of living cells. , 2001, Journal of biomedical optics.

[20]  E. Swanson,et al.  Optical Coherence Tomography , 1992, LEOS '92 Conference Proceedings.

[21]  W. Denk,et al.  Two-photon laser scanning fluorescence microscopy. , 1990, Science.