论文信息 - Depth-resolved fluorescence of biological tissue

Depth-resolved fluorescence of biological tissue

The depth-resolved autofluorescence ofrabbit oral tissue, normal and dysplastic human ectocervical tissue within l20μm depth were investigated utilizing a confocal fluorescence spectroscopy with the excitations at 355nm and 457nm. From the topmost keratinizing layer of oral and ectocervical tissue, strong keratin fluorescence with the spectral characteristics similar to collagen was observed. The fluorescence signal from epithelial tissue between the keratinizing layer and stroma can be well resolved. Furthermore, NADH and FADfluorescence measured from the underlying non-keratinizing epithelial layer were strongly correlated to the tissue pathology. This study demonstrates that the depth-resolved fluorescence spectroscopy can reveal fine structural information on epithelial tissue and potentially provide more accurate diagnostic information for determining tissue pathology.

[1] Peng Xi,et al. Depth-resolved fluorescence spectroscopy reveals layered structure of tissue. , 2004, Optics express.

[2] R. Richards-Kortum,et al. Microanatomical and Biochemical Origins of Normal and Precancerous Cervical Autofluorescence Using Laser-scanning Fluorescence Confocal Microscopy¶ , 2003, Photochemistry and photobiology.

[3] R. Lotan,et al. Autofluorescence Microscopy of Fresh Cervical-Tissue Sections Reveals Alterations in Tissue Biochemistry with Dysplasia¶ , 2001, Photochemistry and photobiology.

[4] Nirmala Ramanujam,et al. Optimal methods for fluorescence and diffuse reflectance measurements of tissue biopsy samples , 2002, Lasers in surgery and medicine.

[5] B. Chance,et al. Oxidation-reduction ratio studies of mitochondria in freeze-trapped samples. NADH and flavoprotein fluorescence signals. , 1979, The Journal of biological chemistry.

[6] E. Sevick-Muraca,et al. Quantitative optical spectroscopy for tissue diagnosis. , 1996, Annual review of physical chemistry.