Heterologous Production of Antimicrobial Peptides: Notes to Consider

[1]  O. Kuipers,et al.  Lipidated variants of the antimicrobial peptide nisin produced via incorporation of methionine analogs for click chemistry show improved bioactivity , 2023, The Journal of biological chemistry.

[2]  Guangshun Wang,et al.  Advances in Antimicrobial Peptide Discovery via Machine Learning and Delivery via Nanotechnology , 2023, Microorganisms.

[3]  S. Hamdan,et al.  Efficient in planta production of amidated antimicrobial peptides that are active against drug-resistant ESKAPE pathogens , 2023, Nature Communications.

[4]  Ming Li,et al.  Heterologous Expression and Bioactivity Determination of Monochamus alternatus Antibacterial Peptide Gene in Komagataella phaffii (Pichia pastoris) , 2023, International journal of molecular sciences.

[5]  M. Tabarzad,et al.  Antimicrobial peptides with anticancer activity: Today status, trends and their computational design. , 2023, Archives of biochemistry and biophysics.

[6]  Ram Shankar Barai,et al.  CAMPR4: a database of natural and synthetic antimicrobial peptides , 2022, Nucleic Acids Res..

[7]  Yanjie Wei,et al.  Editorial: Machine learning for peptide structure, function, and design , 2022, Frontiers in Genetics.

[8]  C. Mora,et al.  Over half of known human pathogenic diseases can be aggravated by climate change , 2022, Nature Climate Change.

[9]  S. Qin,et al.  Two Foreign Antimicrobial Peptides Expressed in the Chloroplast of Porphyridium purpureum Possessed Antibacterial Properties , 2022, Marine drugs.

[10]  Juan C. Cruz,et al.  Rational Discovery of Antimicrobial Peptides by Means of Artificial Intelligence , 2022, Membranes.

[11]  F. Abdulhafiz Plant Cell Culture Technologies: A promising alternatives to Produce High-Value Secondary Metabolites , 2022, Arabian Journal of Chemistry.

[12]  I. Birol,et al.  Mining Amphibian and Insect Transcriptomes for Antimicrobial Peptide Sequences with rAMPage , 2022, Antibiotics.

[13]  O. Bakare,et al.  Plant Antimicrobial Peptides (PAMPs): Features, Applications, Production, Expression, and Challenges , 2022, Molecules.

[14]  Shengyou Huang,et al.  Efficient 3D conformer generation of cyclic peptides formed by a disulfide bond , 2022, Journal of Cheminformatics.

[15]  J. Vederas,et al.  SPI “sandwich”: Combined SUMO‐Peptide‐Intein expression system and isolation procedure for improved stability and yield of peptides , 2022, Protein science : a publication of the Protein Society.

[16]  H. Wieden,et al.  Strategies for improving antimicrobial peptide production. , 2022, Biotechnology advances.

[17]  Ying Lin,et al.  Production of antimicrobial peptide arasin-likeSp in Escherichia coli via an ELP-intein self-cleavage system. , 2022, Journal of biotechnology.

[18]  D. Salzig,et al.  The effect of different insect cell culture media on the efficiency of protein production by Spodoptera frugiperda cells , 2022, Electronic Journal of Biotechnology.

[19]  V. Uversky,et al.  Bioactive Peptides: Synthesis, Sources, Applications, and Proposed Mechanisms of Action , 2022, International journal of molecular sciences.

[20]  J. Rolain,et al.  Application and Challenge of 3rd Generation Sequencing for Clinical Bacterial Studies , 2022, International journal of molecular sciences.

[21]  D. Salzig,et al.  Process intensification for the continuous production of an antimicrobial peptide in stably-transformed Sf-9 insect cells , 2022, Scientific reports.

[22]  Yaoting Gui,et al.  Mutations of MSH5 in nonobstructive azoospermia (NOA) and rescued via in vivo gene editing , 2022, Signal Transduction and Targeted Therapy.

[23]  J. Sohn,et al.  A novel protein fusion partner, carbohydrate-binding module family 66, to enhance heterologous protein expression in Escherichia coli , 2021, Microbial Cell Factories.

[24]  P. Grieco,et al.  Antimicrobial Activity of a Lipidated Temporin L Analogue against Carbapenemase-Producing Klebsiella pneumoniae Clinical Isolates , 2021, Antibiotics.

[25]  F. Sjöberg,et al.  Evaluation of LL‐37 in healing of hard‐to‐heal venous leg ulcers: A multicentric prospective randomized placebo‐controlled clinical trial , 2021, Wound Repair and Regeneration.

[26]  O. Franco,et al.  Dissecting the relationship between antimicrobial peptides and mesenchymal stem cells. , 2021, Pharmacology & therapeutics.

[27]  Owen M. McDougal,et al.  Expression and purification of a cleavable recombinant fortilin from Escherichia coli for structure activity studies. , 2021, Protein expression and purification.

[28]  P. Sahu,et al.  Exploiting endophytic microbes as micro-factories for plant secondary metabolite production , 2021, Applied Microbiology and Biotechnology.

[29]  Shaun W. Lee,et al.  The Role of Bacterial Proteases in Microbe and Host-microbe Interactions. , 2021, Current drug targets.

[30]  I. Frébort,et al.  Long-Lasting Stable Expression of Human LL-37 Antimicrobial Peptide in Transgenic Barley Plants , 2021, Antibiotics.

[31]  Yingmin Xu Phage and phage lysins: New era of bio-preservatives and food safety agents. , 2021, Journal of food science.

[32]  W. Phoolcharoen,et al.  Biotechnological Insights on the Expression and Production of Antimicrobial Peptides in Plants , 2021, Molecules.

[33]  M. Awasthi,et al.  Customized yeast cell factories for biopharmaceuticals: from cell engineering to process scale up , 2021, Microbial Cell Factories.

[34]  P. Falabella,et al.  Antimicrobial Peptides: A New Hope in Biomedical and Pharmaceutical Fields , 2021, Frontiers in Cellular and Infection Microbiology.

[35]  Jiang Xia,et al.  Antimicrobial peptides towards clinical application: delivery and formulation. , 2021, Advanced drug delivery reviews.

[36]  A. Mosavat,et al.  Practical Methods for Expression of Recombinant Protein in the Pichia pastoris System , 2021, Current protocols.

[37]  Zichao Wang,et al.  N‐terminal acetylation of antimicrobial peptide L163 improves its stability against protease degradation , 2021, Journal of peptide science : an official publication of the European Peptide Society.

[38]  R. Halabian,et al.  Design, Dimerization, and Recombinant Production of MCh-AMP1–Derived Peptide in Escherichia coli and Evaluation of Its Antifungal Activity and Cytotoxicity , 2021, Frontiers in Fungal Biology.

[39]  Kieran M Lynch,et al.  Future of antimicrobial peptides derived from plants in food application – A focus on synthetic peptides , 2021 .

[40]  C. Creevey,et al.  The rumen eukaryotome is a source of novel antimicrobial peptides with therapeutic potential , 2021, BMC microbiology.

[41]  M. Yusof,et al.  Plant Growth-Promoting Bacteria as an Emerging Tool to Manage Bacterial Rice Pathogens , 2021, Microorganisms.

[42]  Yi Yan Yang,et al.  Accelerated antimicrobial discovery via deep generative models and molecular dynamics simulations , 2021, Nature Biomedical Engineering.

[43]  E. Middelkoop,et al.  Review: Lessons Learned From Clinical Trials Using Antimicrobial Peptides (AMPs) , 2021, Frontiers in Microbiology.

[44]  Jacqueline R. M. A. Maasch,et al.  Molecular Dynamics for Antimicrobial Peptide Discovery , 2021, Infection and Immunity.

[45]  F. Albericio,et al.  Liquid Phase Peptide Synthesis via One‐Pot Nanostar Sieving (PEPSTAR) , 2021, Angewandte Chemie.

[46]  Joanna Tkaczewska Peptides and protein hydrolysates as food preservatives and bioactive components of edible films and coatings - A review , 2020 .

[47]  Alex Rosenthal,et al.  DBAASP v3: database of antimicrobial/cytotoxic activity and structure of peptides as a resource for development of new therapeutics , 2020, Nucleic Acids Res..

[48]  H. Cao,et al.  Acinetobacter venetianus, a potential pathogen of red leg disease in freshwater-cultured whiteleg shrimp Penaeus vannamei , 2020 .

[49]  M. Khatun,et al.  Extract of neem (Azadirachta indica) leaf exhibits bactericidal effect against multidrug resistant pathogenic bacteria of poultry , 2020, Veterinary medicine and science.

[50]  Q. Kong,et al.  Antimicrobial Peptides: Classification, Design, Application and Research Progress in Multiple Fields , 2020, Frontiers in Microbiology.

[51]  Yongping Huang,et al.  Transgenic expression of antimicrobial peptides from black soldier fly enhance resistance against entomopathogenic bacteria in the silkworm, Bombyx mori. , 2020, Insect biochemistry and molecular biology.

[52]  Zaida Luthey-Schulten,et al.  Generalized correlation-based dynamical network analysis: a new high-performance approach for identifying allosteric communications in molecular dynamics trajectories , 2020, bioRxiv.

[53]  W. Hozzein,et al.  Emerging MDR-Pseudomonas aeruginosa in fish commonly harbor oprL and toxA virulence genes and blaTEM, blaCTX-M, and tetA antibiotic-resistance genes , 2020, Scientific Reports.

[54]  T. H. Tran,et al.  Antimicrobial peptides – Advances in development of therapeutic applications , 2020, Life Sciences.

[55]  Jang-Su Park,et al.  Strategies for Optimizing the Production of Proteins and Peptides with Multiple Disulfide Bonds , 2020, Antibiotics.

[56]  F. Nazarian-Firouzabadi,et al.  New Recombinant Antimicrobial Peptides Confer Resistance to Fungal Pathogens in Tobacco Plants , 2020, Frontiers in Plant Science.

[57]  Ming-hao Wu,et al.  Effects of glycosylation and D-amino acid substitution on the antitumor and antibacterial activities of bee venom peptide HYL. , 2020, Bioconjugate chemistry.

[58]  Yi Wang,et al.  Scalable molecular dynamics on CPU and GPU architectures with NAMD. , 2020, The Journal of chemical physics.

[59]  Thanos Papadopoulos,et al.  The use of digital technologies by small and medium enterprises during COVID-19: Implications for theory and practice , 2020, International Journal of Information Management.

[60]  Tianzhong Liu,et al.  Chloroplast Genetic Engineering of a Unicellular Green Alga Haematococcus pluvialis with Expression of an Antimicrobial Peptide , 2020, Marine Biotechnology.

[61]  A. Shan,et al.  Heterologous expression of the novel α-helical hybrid peptide PR-FO in Bacillus subtilis , 2020, Bioprocess and Biosystems Engineering.

[62]  R. Duval,et al.  Bacteriocins, Antimicrobial Peptides from Bacterial Origin: Overview of Their Biology and Their Impact against Multidrug-Resistant Bacteria , 2020, Microorganisms.

[63]  Hemant Kumar Srivastava,et al.  Peptide-like and small-molecule inhibitors against Covid-19 , 2020, Journal of biomolecular structure & dynamics.

[64]  M. B. Burnett,et al.  Therapeutic recombinant protein production in plants: Challenges and opportunities , 2020 .

[65]  Jorng-Tzong Horng,et al.  Characterization and Identification of Natural Antimicrobial Peptides on Different Organisms , 2020, International journal of molecular sciences.

[66]  R. Mikut,et al.  The effect of lipidation and glycosylation on short cationic antimicrobial peptides. , 2020, Biochimica et biophysica acta. Biomembranes.

[67]  T. Lu,et al.  Development and Challenges of Antimicrobial Peptides for Therapeutic Applications , 2020, Antibiotics.

[68]  S. Sardari,et al.  Antimicrobial peptides of the vaginal innate immunity and their role in the fight against sexually transmitted diseases , 2019, New microbes and new infections.

[69]  A. Tossi,et al.  Antimicrobial Peptides as Anti-Infective Agents in Pre-Post-Antibiotic Era? , 2019, International journal of molecular sciences.

[70]  P. Czermak,et al.  Selection of High Producers From Combinatorial Libraries for the Production of Recombinant Proteins in Escherichia coli and Vibrio natriegens , 2019, Front. Bioeng. Biotechnol..

[71]  Jiwon Seo,et al.  Antimicrobial peptides under clinical investigation , 2019, Peptide Science.

[72]  D. Craik,et al.  Characterization of Tachyplesin Peptides and Their Cyclized Analogues to Improve Antimicrobial and Anticancer Properties , 2019, International journal of molecular sciences.

[73]  Cheng Shi,et al.  DRAMP 2.0, an updated data repository of antimicrobial peptides , 2019, Scientific Data.

[74]  B. Gyurcsik,et al.  Purification of proteins with native terminal sequences using a Ni(II)-cleavable C-terminal hexahistidine affinity tag. , 2019, Protein expression and purification.

[75]  D. Craik,et al.  The Vast Structural Diversity of Antimicrobial Peptides. , 2019, Trends in pharmacological sciences.

[76]  M. Hewicker-Trautwein,et al.  Host-Pathogen Interactions of Mycoplasma mycoides in Caprine and Bovine Precision-Cut Lung Slices (PCLS) Models , 2019, Pathogens.

[77]  Alireza Neshani,et al.  Epinecidin-1, a highly potent marine antimicrobial peptide with anticancer and immunomodulatory activities , 2019, BMC Pharmacology and Toxicology.

[78]  F. Gallou,et al.  Sustainability Challenges in Peptide Synthesis and Purification: From R&D to Production. , 2019, The Journal of organic chemistry.

[79]  S. Islam,et al.  Making plants into cost-effective bioreactors for highly active antimicrobial peptides , 2019, bioRxiv.

[80]  João Paulo Teixeira,et al.  Self-disinfecting surfaces and infection control , 2019, Colloids and Surfaces B: Biointerfaces.

[81]  Kwang-Chul Kwon,et al.  A new prokaryotic expression vector for the expression of antimicrobial peptide abaecin using SUMO fusion tag , 2019, BMC Biotechnology.

[82]  R. Sinha,et al.  Antimicrobial Peptides: Recent Insights on Biotechnological Interventions and Future Perspectives , 2019, Protein and peptide letters.

[83]  Patrick Fickers,et al.  Bioreactor-Scale Strategies for the Production of Recombinant Protein in the Yeast Yarrowia lipolytica , 2019, Microorganisms.

[84]  M. Brimble,et al.  Using chemical synthesis to optimise antimicrobial peptides in the fight against antimicrobial resistance , 2019, Pure and Applied Chemistry.

[85]  Xiaoqing Shi,et al.  Efficient production of antifungal proteins in plants using a new transient expression vector derived from tobacco mosaic virus , 2018, Plant biotechnology journal.

[86]  D. Drechsel,et al.  FlexiBAC: a versatile, open-source baculovirus vector system for protein expression, secretion, and proteolytic processing , 2018, bioRxiv.

[87]  K. Kuča,et al.  Insect Antimicrobial Peptides, a Mini Review , 2018, Toxins.

[88]  Tzong-Yi Lee,et al.  dbAMP: an integrated resource for exploring antimicrobial peptides with functional activities and physicochemical properties on transcriptome and proteome data , 2018, Nucleic Acids Res..

[89]  S. Woo,et al.  Enhanced Production of Recombinant Protein by Fusion Expression with Ssp DnaB Mini-Intein in the Baculovirus Expression System , 2018, Viruses.

[90]  G. Sundin,et al.  Antibiotic Resistance in Plant-Pathogenic Bacteria. , 2018, Annual review of phytopathology.

[91]  A. Rasmusson,et al.  The antibiotic peptaibol alamethicin from Trichoderma permeabilises Arabidopsis root apical meristem and epidermis but is antagonised by cellulase-induced resistance to alamethicin , 2018, BMC Plant Biology.

[92]  Oscar P. Kuipers,et al.  BAGEL4: a user-friendly web server to thoroughly mine RiPPs and bacteriocins , 2018, Nucleic Acids Res..

[93]  S. Sirikantaramas,et al.  Heterologous expression and antimicrobial activity of OsGASR3 from rice (Oryza sativa L.). , 2018, Journal of plant physiology.

[94]  N. Parachin,et al.  Comparison of Yeasts as Hosts for Recombinant Protein Production , 2018, Microorganisms.

[95]  E. Genersch,et al.  Bacterial pathogens of bees. , 2018, Current opinion in insect science.

[96]  Adam J. Stevens,et al.  Improved protein splicing using embedded split inteins , 2018, Protein science : a publication of the Protein Society.

[97]  Min Zhang,et al.  Expression of a recombinant hybrid antimicrobial peptide magainin II-cecropin B in the mycelium of the medicinal fungus Cordyceps militaris and its validation in mice , 2018, Microbial Cell Factories.

[98]  A. Fazeli,et al.  Recombinant production of bovine Lactoferrin-derived antimicrobial peptide in tobacco hairy roots expression system. , 2018, Plant physiology and biochemistry : PPB.

[99]  T. Teeri,et al.  A Gene Encoding Scots Pine Antimicrobial Protein Sp-AMP2 (PR-19) Confers Increased Tolerance against Botrytis cinerea in Transgenic Tobacco , 2017 .

[100]  Andrew L. Ferguson,et al.  What can machine learning do for antimicrobial peptides, and what can antimicrobial peptides do for machine learning? , 2017, Interface Focus.

[101]  C. Zhai,et al.  The heterologous expression strategies of antimicrobial peptides in microbial systems. , 2017, Protein expression and purification.

[102]  O. Taboga,et al.  Successful production of the potato antimicrobial peptide Snakin-1 in baculovirus-infected insect cells and development of specific antibodies , 2017, BMC Biotechnology.

[103]  Mingtao Huang,et al.  Efficient protein production by yeast requires global tuning of metabolism , 2017, Nature Communications.

[104]  G. Zhong,et al.  Transcriptome analysis of Spodoptera frugiperda Sf9 cells reveals putative apoptosis-related genes and a preliminary apoptosis mechanism induced by azadirachtin , 2017, Scientific Reports.

[105]  Joël Richard Challenges in oral peptide delivery: lessons learnt from the clinic and future prospects. , 2017, Therapeutic delivery.

[106]  Chuan-fa Liu,et al.  Progress in Chemical Synthesis of Peptides and Proteins , 2017 .

[107]  T. Mirski,et al.  Utilisation of peptides against microbial infections - a review. , 2017, Annals of agricultural and environmental medicine : AAEM.

[108]  C. Millán-Pacheco,et al.  Streptomyces as Overexpression System for Heterologous Production of an Antimicrobial Peptide. , 2017, Protein and peptide letters.

[109]  V. Giaccone,et al.  Antimicrobial resistance: A global emerging threat to public health systems , 2017, Critical reviews in food science and nutrition.

[110]  L. Goulart,et al.  Heterologous expression of abaecin peptide from Apis mellifera in Pichia pastoris , 2017, Microbial Cell Factories.

[111]  D. Kuzmin,et al.  Effect of N- and C-Terminal Modifications on Cytotoxic Properties of Antimicrobial Peptide Tachyplesin I , 2017, Bulletin of Experimental Biology and Medicine.

[112]  D. Salzig,et al.  A high-throughput expression screening platform to optimize the production of antimicrobial peptides , 2017, Microbial Cell Factories.

[113]  G. Pazour,et al.  Ror2 signaling regulates Golgi structure and transport through IFT20 for tumor invasiveness , 2017, Scientific Reports.

[114]  Karen G. N. Oshiro,et al.  Antimicrobial Peptides from Fruits and Their Potential Use as Biotechnological Tools—A Review and Outlook , 2017, Front. Microbiol..

[115]  Ziniu Yu,et al.  Screening, Expression, Purification and Functional Characterization of Novel Antimicrobial Peptide Genes from Hermetia illucens (L.) , 2017, PloS one.

[116]  Q. Gu,et al.  Heterologous expression and purification of plantaricin NC8, a two-peptide bacteriocin against Salmonella spp. from Lactobacillus plantarum ZJ316. , 2016, Protein expression and purification.

[117]  Zhen‐Chuan Fan,et al.  Expression, purification and initial characterization of a novel recombinant antimicrobial peptide Mytichitin-A in Pichia pastoris. , 2016, Protein expression and purification.

[118]  Shi Tian,et al.  Recombinant expression and biological characterization of the antimicrobial peptide fowlicidin-2 in Pichia pastoris. , 2016, Experimental and therapeutic medicine.

[119]  H. Daniell,et al.  Topical delivery of low-cost protein drug candidates made in chloroplasts for biofilm disruption and uptake by oral epithelial cells. , 2016, Biomaterials.

[120]  M. Rai,et al.  Antimicrobial peptides as natural bio-preservative to enhance the shelf-life of food , 2016, Journal of Food Science and Technology.

[121]  H. Vogel,et al.  Overexpression of Antimicrobial, Anticancer, and Transmembrane Peptides in Escherichia coli through a Calmodulin-Peptide Fusion System. , 2016, Journal of the American Chemical Society.

[122]  M. V. van Hoek,et al.  Peptides from American alligator plasma are antimicrobial against multi-drug resistant bacterial pathogens including Acinetobacter baumannii , 2016, BMC Microbiology.

[123]  C. Jeon,et al.  High-throughput recombinant protein expression in Escherichia coli: current status and future perspectives , 2016, Open Biology.

[124]  T. Kikukawa,et al.  Expression, purification and characterization of the recombinant cysteine-rich antimicrobial peptide snakin-1 in Pichia pastoris. , 2016, Protein expression and purification.

[125]  Eugene I. Rumynskiy,et al.  A novel lipid transfer protein from the pea Pisum sativum: isolation, recombinant expression, solution structure, antifungal activity, lipid binding, and allergenic properties , 2016, BMC Plant Biology.

[126]  M. Varcamonti,et al.  Rational Design of a Carrier Protein for the Production of Recombinant Toxic Peptides in Escherichia coli , 2016, PloS one.

[127]  P. White,et al.  Advances in Fmoc solid‐phase peptide synthesis , 2016, Journal of peptide science : an official publication of the European Peptide Society.

[128]  Xia Li,et al.  APD3: the antimicrobial peptide database as a tool for research and education , 2015, Nucleic Acids Res..

[129]  Andrei Gabrielian,et al.  DBAASP v.2: an enhanced database of structure and antimicrobial/cytotoxic activity of natural and synthetic peptides , 2015, Nucleic acids research.

[130]  Faiza Hanif Waghu,et al.  CAMPR3: a database on sequences, structures and signatures of antimicrobial peptides , 2015, Nucleic Acids Res..

[131]  T. Kohchi,et al.  Development of Gateway Binary Vector Series with Four Different Selection Markers for the Liverwort Marchantia polymorpha , 2015, PloS one.

[132]  Berk Hess,et al.  GROMACS: High performance molecular simulations through multi-level parallelism from laptops to supercomputers , 2015 .

[133]  Francesca D'Este,et al.  Antifungal activity of cathelicidin peptides against planktonic and biofilm cultures of Candida species isolated from vaginal infections , 2015, Peptides.

[134]  Xiaoling Chen,et al.  Characterization of bioactive recombinant antimicrobial peptide parasin I fused with human lysozyme expressed in the yeast Pichia pastoris system. , 2015, Enzyme and microbial technology.

[135]  M. Wink,et al.  Recombinant Production of Snakin-2 (an Antimicrobial Peptide from Tomato) in E. coli and Analysis of Its Bioactivity , 2015, Molecules.

[136]  Yu-Jin Jung,et al.  HETEROLOGOUS EXPRESSION OF ANTIMICROBIAL PEPTIDE LL-37 IN CHINESE CABBAGE WITH ENHANCED RESISTANCE TO PATHOGENS , 2015 .

[137]  B. Yip,et al.  Novel Antimicrobial Peptides with High Anticancer Activity and Selectivity , 2015, PloS one.

[138]  Kuan Y. Chang,et al.  A Large-Scale Structural Classification of Antimicrobial Peptides , 2015, BioMed research international.

[139]  J. Wiesner,et al.  Two c-type lysozymes boost the innate immune system of the invasive ladybird Harmonia axyridis. , 2015, Developmental and comparative immunology.

[140]  Xintao Cao,et al.  Optimization of expression conditions for a novel NZ2114-derived antimicrobial peptide-MP1102 under the control of the GAP promoter in Pichia pastoris X-33 , 2015, BMC Microbiology.

[141]  A. Kralicek,et al.  Expression and purification of the antimicrobial peptide GSL1 in bacteria for raising antibodies , 2014, BMC Research Notes.

[142]  C. Hou,et al.  Production of antibacterial peptide from bee venom via a new strategy for heterologous expression , 2014, Molecular Biology Reports.

[143]  A. Beck‐Sickinger,et al.  Automated solid-phase peptide synthesis to obtain therapeutic peptides , 2014, Beilstein journal of organic chemistry.

[144]  Zhengxiang Wang,et al.  Influence of promoter and signal peptide on the expression of pullulanase in Bacillus subtilis , 2014, Biotechnology Letters.

[145]  Germán L. Rosano,et al.  Recombinant protein expression in Escherichia coli: advances and challenges , 2014, Front. Microbiol..

[146]  P. Pelegrini,et al.  Antifungal defensins and their role in plant defense , 2014, Front. Microbiol..

[147]  E. Grishin,et al.  A novel hairpin-like antimicrobial peptide from barnyard grass (Echinochloa crusgalli L.) seeds: Structure-functional and molecular-genetics characterization. , 2014, Biochimie.

[148]  K. Ram,et al.  Cost effective purification of intein based syntetic cationic antimicrobial peptide expressed in cold shock expression system using salt inducible E. coli GJ1158 , 2014 .

[149]  Yue‐wen Chen,et al.  HETEROLOGOUS EXPRESSION AND PURIFICATION OF DERMASEPTIN S4 FUSION IN Escherichia coli AND RECOVERY OF BIOLOGICAL ACTIVITY , 2014, Preparative biochemistry & biotechnology.

[150]  R. Hancock,et al.  Expression of an Engineered Heterologous Antimicrobial Peptide in Potato Alters Plant Development and Mitigates Normal Abiotic and Biotic Responses , 2013, PloS one.

[151]  K. Mineev,et al.  Recombinant production and solution structure of lipid transfer protein from lentil Lens culinaris. , 2013, Biochemical and biophysical research communications.

[152]  D. Tang,et al.  Expression of antimicrobial peptides thanatin(S) in transgenic Arabidopsis enhanced resistance to phytopathogenic fungi and bacteria. , 2013, Gene.

[153]  Christopher T Lohans,et al.  Structural characterization of thioether-bridged bacteriocins , 2013, The Journal of Antibiotics.

[154]  M. Ueda,et al.  Characterization of Antimicrobial Peptides toward the Development of Novel Antibiotics , 2013, Pharmaceuticals.

[155]  C. Walsh,et al.  Nonproteinogenic amino acid building blocks for nonribosomal peptide and hybrid polyketide scaffolds. , 2013, Angewandte Chemie.

[156]  Yu-Jin Jung Enhanced resistance to bacterial pathogen in transgenic tomato plants expressing cathelicidin antimicrobial peptide , 2013, Biotechnology and Bioprocess Engineering.

[157]  S. Korban,et al.  Transient expression and characterization of the antimicrobial peptide protegrin-1 in Nicotiana tabacum for control of bacterial and fungal mammalian pathogens , 2013, Plant Cell, Tissue and Organ Culture (PCTOC).

[158]  Xiaowei Zhao,et al.  LAMP: A Database Linking Antimicrobial Peptides , 2013, PloS one.

[159]  A. Arseniev,et al.  Recombinant expression and solution structure of antimicrobial peptide aurelin from jellyfish Aurelia aurita. , 2012, Biochemical and biophysical research communications.

[160]  O. Franco,et al.  Expression systems for heterologous production of antimicrobial peptides , 2012, Peptides.

[161]  L. Tavares,et al.  Antimicrobial activity of recombinant Pg-AMP1, a glycine-rich peptide from guava seeds , 2012, Peptides.

[162]  J. Ueda,et al.  Transgenic tobacco plants expressing antimicrobial peptide bovine lactoferricin show enhanced resistance to phytopathogens , 2012 .

[163]  Jianfeng Xu,et al.  Green factory: plants as bioproduction platforms for recombinant proteins. , 2012, Biotechnology advances.

[164]  Davor Juretic,et al.  DADP: the database of anuran defense peptides , 2012, Bioinform..

[165]  H. V. van Leeuwen,et al.  The Human Lactoferrin-Derived Peptide hLF1-11 Exerts Immunomodulatory Effects by Specific Inhibition of Myeloperoxidase Activity , 2012, The Journal of Immunology.

[166]  Jens Nielsen,et al.  Different expression systems for production of recombinant proteins in Saccharomyces cerevisiae , 2012, Biotechnology and bioengineering.

[167]  S. Piotto,et al.  YADAMP: yet another database of antimicrobial peptides. , 2012, International journal of antimicrobial agents.

[168]  Ke‐jian Wang,et al.  Optimized production of scygonadin in Pichia pastoris and analysis of its antimicrobial and antiviral activities. , 2012, Protein expression and purification.

[169]  R. Soria-Guerra,et al.  Chlamydomonas reinhardtii as a viable platform for the production of recombinant proteins: current status and perspectives , 2012, Plant Cell Reports.

[170]  E. Lentz,et al.  Stacking of antimicrobial genes in potato transgenic plants confers increased resistance to bacterial and fungal pathogens. , 2012, Journal of biotechnology.

[171]  N. Mitsukawa,et al.  Transgenic sweet potato expressing thionin from barley gives resistance to black rot disease caused by Ceratocystis fimbriata in leaves and storage roots , 2012, Plant Cell Reports.

[172]  Guangshun Wang Post-translational Modifications of Natural Antimicrobial Peptides and Strategies for Peptide Engineering. , 2011, Current biotechnology.

[173]  Yifeng Li Recombinant production of antimicrobial peptides in Escherichia coli: a review. , 2011, Protein expression and purification.

[174]  Chin-Fu Chen,et al.  Expression of a Novel Antimicrobial Peptide Penaeidin4-1 in Creeping Bentgrass (Agrostis stolonifera L.) Enhances Plant Fungal Disease Resistance , 2011, PloS one.

[175]  R. Pogni,et al.  Structural and Functional Consequences Induced by Post-Translational Modifications in α-Defensins , 2011, International journal of peptides.

[176]  D. Teng,et al.  Expression of plectasin in Pichia pastoris and its characterization as a new antimicrobial peptide against Staphyloccocus and Streptococcus. , 2011, Protein expression and purification.

[177]  Zhanglin Lin,et al.  Streamlined protein expression and purification using cleavable self-aggregating tags , 2011, Microbial cell factories.

[178]  A. Vilcinskas Anti-infective therapeutics from the Lepidopteran model host Galleria mellonella. , 2011, Current pharmaceutical design.

[179]  Z. Li,et al.  Expression of a radish defensin in transgenic wheat confers increased resistance to Fusarium graminearum and Rhizoctonia cerealis , 2011, Functional & Integrative Genomics.

[180]  F. Krens,et al.  Performance and long-term stability of the barley hordothionin gene in multiple transgenic apple lines , 2011, Transgenic Research.

[181]  R. Khan,et al.  Stable integration and expression of wasabi defensin gene in “Egusi” melon (Colocynthis citrullus L.) confers resistance to Fusarium wilt and Alternaria leaf spot , 2010, Plant Cell Reports.

[182]  Wen-shu Huang,et al.  Soluble expression and purification of a crab antimicrobial peptide scygonadin in different expression plasmids and analysis of its antimicrobial activity. , 2010, Protein expression and purification.

[183]  R. Hammami,et al.  BACTIBASE second release: a database and tool platform for bacteriocin characterization , 2010, BMC Microbiology.

[184]  B. Altincicek,et al.  Insect peptide metchnikowin confers on barley a selective capacity for resistance to fungal ascomycetes pathogens , 2009, Journal of experimental botany.

[185]  K. P. Pauls,et al.  PDC1, a corn defensin peptide expressed in Escherichia coli and Pichia pastoris inhibits growth of Fusarium graminearum , 2009, Peptides.

[186]  J. Beutler,et al.  Natural Products as a Foundation for Drug Discovery , 2009, Current protocols in pharmacology.

[187]  Yul-Ho Kim,et al.  Expression of BrD1, a plant defensin from Brassica rapa, confers resistance against brown planthopper (Nilaparvata lugens) in transgenic rices , 2009, Molecules and cells.

[188]  E. Grishin,et al.  A novel antifungal hevein‐type peptide from Triticum kiharae seeds with a unique 10‐cysteine motif , 2009, The FEBS journal.

[189]  Tony Pawson,et al.  Comparative Analysis Reveals Conserved Protein Phosphorylation Networks Implicated in Multiple Diseases , 2009, Science Signaling.

[190]  Shuang-quan Zhang,et al.  TrxA mediating fusion expression of antimicrobial peptide CM4 from multiple joined genes in Escherichia coli. , 2009, Protein expression and purification.

[191]  B. Simon,et al.  Screening of fusion partners for high yield expression and purification of bioactive viscotoxins. , 2009, Protein expression and purification.

[192]  Shuang-quan Zhang,et al.  Expression and purification the antimicrobial peptide CM4 in Escherichia coli , 2009, Biotechnology Letters.

[193]  Riadh Hammami,et al.  PhytAMP: a database dedicated to antimicrobial plant peptides , 2008, Nucleic Acids Res..

[194]  R. Syvitski,et al.  Expression, purification and structural characterization of recombinant hepcidin, an antimicrobial peptide identified in Japanese flounder, Paralichthys olivaceus. , 2008, Protein expression and purification.

[195]  I. Vasil A history of plant biotechnology: from the Cell Theory of Schleiden and Schwann to biotech crops , 2008, Plant Cell Reports.

[196]  K. Nigútová,et al.  Heterologous expression of functionally active enterolysin A, class III bacteriocin from Enterococcus faecalis, in Escherichia coli. , 2008, Protein expression and purification.

[197]  H. Hopp,et al.  Overexpression of snakin-1 gene enhances resistance to Rhizoctonia solani and Erwinia carotovora in transgenic potato plants. , 2008, Molecular plant pathology.

[198]  T. Meinnel,et al.  Protein lipidation meets proteomics. , 2008, Frontiers in bioscience : a journal and virtual library.

[199]  Shengwang Liu,et al.  Expression and characterization of recombinant gallinacin-9 and gallinacin-8 in Escherichia coli. , 2008, Protein expression and purification.

[200]  U. Oppermann,et al.  Ligand supplementation as a method to increase soluble heterologous protein production , 2008, Expert review of proteomics.

[201]  I. Hirono,et al.  Molecular cloning, genomic organization and recombinant expression of a crustin-like antimicrobial peptide from black tiger shrimp Penaeus monodon. , 2008, Molecular immunology.

[202]  J. Brusslan,et al.  Plant peptides and peptidomics. , 2008, Plant biotechnology journal.

[203]  F. Castiglione,et al.  Determining the structure and mode of action of microbisporicin, a potent lantibiotic active against multiresistant pathogens. , 2008, Chemistry & biology.

[204]  H. Daniell,et al.  Chloroplast Vector Systems for Biotechnology Applications1 , 2007, Plant Physiology.

[205]  M. Subirade,et al.  Production of active pediocin PA-1 in Escherichia coli using a thioredoxin gene fusion expression approach: cloning, expression, purification, and characterization. , 2007, Canadian journal of microbiology.

[206]  D. Yevtushenko,et al.  Comparison of pathogen-induced expression and efficacy of two amphibian antimicrobial peptides, MsrA2 and temporin A, for engineering wide-spectrum disease resistance in tobacco. , 2007, Plant biotechnology journal.

[207]  Y. Niwa,et al.  Development of series of gateway binary vectors, pGWBs, for realizing efficient construction of fusion genes for plant transformation. , 2007, Journal of bioscience and bioengineering.

[208]  P. Hernández,et al.  Cloning, production and expression of the bacteriocin enterocin A produced by Enterococcus faecium PLBC21 in Lactococcus lactis , 2007, Applied Microbiology and Biotechnology.

[209]  Tao Xi,et al.  High-Level Production of a Novel Antimicrobial Peptide Perinerin in Escherichia coli by Fusion Expression , 2007, Current Microbiology.

[210]  C. Fjell,et al.  AMPer: a database and an automated discovery tool for antimicrobial peptides , 2007, Bioinform..

[211]  M. Martínez-Bueno,et al.  Heterologous expression of enterocin AS‐48 in several strains of lactic acid bacteria , 2007, Journal of applied microbiology.

[212]  B. Terrier,et al.  Two new disposable bioreactors for plant cell culture: The wave and undertow bioreactor and the slug bubble bioreactor , 2007, Biotechnology and bioengineering.

[213]  B. Cammue,et al.  Arabidopsis thaliana plants expressing human beta-defensin-2 are more resistant to fungal attack: functional homology between plant and human defensins , 2007, Plant Cell Reports.

[214]  R. Hancock,et al.  Antimicrobial and host-defense peptides as new anti-infective therapeutic strategies , 2006, Nature Biotechnology.

[215]  Roger Beuerman,et al.  Defensins knowledgebase: a manually curated database and information source focused on the defensins family of antimicrobial peptides , 2006, Nucleic Acids Res..

[216]  Shin-Geon Choi,et al.  Recombinant expression of human cathelicidin (hCAP18/LL-37) in Pichia pastoris , 2006, Biotechnology Letters.

[217]  M. Nishihara,et al.  Transgenic potatoes expressing wasabi defensin peptide confer partial resistance to gray mold (Botrytis cinerea) , 2006 .

[218]  Chinpan Chen,et al.  Roles of N-terminal pyroglutamate in maintaining structural integrity and pKa values of catalytic histidine residues in bullfrog ribonuclease 3. , 2006, Journal of molecular biology.

[219]  I. Cipakova,et al.  Production of the human-beta-defensin using Saccharomyces cerevisiae as a host. , 2005, Protein and peptide letters.

[220]  S. Marillonnet,et al.  Magnifection--a new platform for expressing recombinant vaccines in plants. , 2005, Vaccine.

[221]  Lei Li,et al.  High level expression, purification, and characterization of the shrimp antimicrobial peptide, Ch-penaeidin, in Pichia pastoris. , 2005, Protein expression and purification.

[222]  M. Klocke,et al.  Heterologous expression of enterocin A, a bacteriocin from Enterococcus faecium, fused to a cellulose-binding domain in Escherichia coli results in a functional protein with inhibitory activity against Listeria , 2005, Applied Microbiology and Biotechnology.

[223]  R. Reski,et al.  The moss bioreactor. , 2004, Current opinion in plant biology.

[224]  M. Zasloff,et al.  Hagfish intestinal antimicrobial peptides are ancient cathelicidins , 2003, Peptides.

[225]  Robert Finking,et al.  Biosynthesis of nonribosomal peptides , 2003 .

[226]  A. Itoh,et al.  Heterologous expression of gassericin A, a bacteriocin produced by Lactobacillus gasseri LA39 , 2003 .

[227]  D. Zheleva,et al.  Liquid‐phase peptide synthesis on polyethylene glycol (PEG) supports using strategies based on the 9‐fluorenylmethoxycarbonyl amino protecting group: application of PEGylated peptides in biochemical assays , 2002, Journal of peptide science : an official publication of the European Peptide Society.

[228]  F. Smith,et al.  Expression of an antimicrobial peptide via the chloroplast genome to control phytopathogenic bacteria and fungi. , 2001, Plant physiology.

[229]  P. Cohen The role of protein phosphorylation in human health and disease. The Sir Hans Krebs Medal Lecture. , 2001, European journal of biochemistry.

[230]  K. Shimazaki,et al.  Production of recombinant bovine lactoferrin N-lobe in insect cells and its antimicrobial activity. , 2001, Protein expression and purification.

[231]  S. Ishii,et al.  Increase of Solubility of Foreign Proteins in Escherichia coli by Coproduction of the Bacterial Thioredoxin (*) , 1995, The Journal of Biological Chemistry.

[232]  OUP accepted manuscript , 2021, Nucleic Acids Research.

[233]  Yini Huang,et al.  Recombinant expression of antimicrobial peptides in Pichia pastoris: A strategy to inhibit the Penicillium expansum in pears , 2021 .

[234]  Manoj Kumar,et al.  Phyto-Microbiome in Stress Regulation , 2020 .

[235]  J. F. Morales-Domínguez,et al.  Heterologous expression of bacteriocin E-760 in Chlamydomonas reinhardtii and functional analysis , 2019, Phyton.

[236]  D. Jaradat Thirteen decades of peptide synthesis: key developments in solid phase peptide synthesis and amide bond formation utilized in peptide ligation , 2017, Amino Acids.

[237]  P. Panteleev,et al.  Improved strategy for recombinant production and purification of antimicrobial peptide tachyplesin I and its analogs with high cell selectivity , 2017, Biotechnology and applied biochemistry.

[238]  D. Salzig,et al.  Considerations for the process development of insect‐derived antimicrobial peptide production , 2015, Biotechnology progress.

[239]  E. Grishin,et al.  Novel antifungal α-hairpinin peptide from Stellaria media seeds: structure, biosynthesis, gene structure and evolution , 2013, Plant Molecular Biology.

[240]  S. Rosales-Mendoza,et al.  Production of milk-derived bioactive peptides as precursor chimeric proteins in chloroplasts of Chlamydomonas reinhardtii , 2012, Plant Cell, Tissue and Organ Culture (PCTOC).

[241]  M. F. Grossi-de-Sá,et al.  Plant biofarming: Novel insights for peptide expression in heterologous systems , 2012, Biopolymers.

[242]  A. Vilcinskas,et al.  Defense gene expression is potentiated in transgenic barley expressing antifungal peptide metchnikowin throughout powdery mildew challenge , 2011, Journal of Plant Research.

[243]  Yanguang Cong,et al.  Recombinant antimicrobial peptide hPAB-β expressed in Pichia pastoris, a potential agent active against methicillin-resistant Staphylococcus aureus , 2010, Applied Microbiology and Biotechnology.

[244]  Xiao-qiang Yu,et al.  Expression and characterization of antimicrobial peptide CecropinAD in the methylotrophic yeast Pichia pastoris , 2009 .

[245]  I. Hirono,et al.  Cloning, expression and antimicrobial activity of crustinPm1, a major isoform of crustin, from the black tiger shrimp Penaeus monodon. , 2008, Developmental and comparative immunology.

[246]  R. Reski,et al.  Current achievements in the production of complex biopharmaceuticals with moss bioreactors , 2008, Bioprocess and biosystems engineering.

[247]  P. Sautière,et al.  Hedistin: A novel antimicrobial peptide containing bromotryptophan constitutively expressed in the NK cells-like of the marine annelid, Nereis diversicolor. , 2007, Developmental and comparative immunology.

[248]  M. Simon,et al.  Production of a recombinant bacterial lipoprotein in higher plant chloroplasts , 2006, Nature Biotechnology.

[249]  Zhinan Xu,et al.  High‐Level Expression of a Soluble Functional Antimicrobial Peptide, Human β‐Defensin 2, in Escherichia coli , 2006, Biotechnology progress.

[250]  Isabelle Mougenot,et al.  PenBase, the shrimp antimicrobial peptide penaeidin database: sequence-based classification and recommended nomenclature. , 2006, Developmental and comparative immunology.

[251]  W. Gong,et al.  cDNA cloning, functional expression and antifungal activities of a dimeric plant defensin SPE10 from Pachyrrhizus erosus seeds , 2004, Plant Molecular Biology.

[252]  W. Chan,et al.  Fmoc solid phase peptide synthesis : a practical approach , 2000 .