Edible nanoemulsions: fabrication, properties, and functional performance

There is increasing interest within the food, beverage and pharmaceutical industries in utilizing edible nanoemulsions to encapsulate, protect and deliver lipophilic functional components, such as oil-soluble flavors, vitamins, preservatives, nutraceuticals, and drugs. There are a number of potential advantages of using nanoemulsions rather than conventional emulsions for this purpose: they can greatly increase the bioavailability of lipophilic substances; they scatter light weakly and so can be incorporated into optically transparent products; they can be used to modulate the product texture; and they have a high stability to particle aggregation and gravitational separation. On the other hand, there may also be some risks associated with the oral ingestion of nanoemulsions, such as their ability to change the biological fate of bioactive components within the gastrointestinal tract and the potential toxicity of some of the components used in their fabrication. This tutorial review provides an overview of the current status of nanoemulsion fabrication, properties, and applications with special emphasis on systems suitable for utilization within the food industry.

[1]  E. Acosta Bioavailability of nanoparticles in nutrient and nutraceutical delivery , 2009 .

[2]  D. Mcclements,et al.  Influence of Environmental Stresses on O/W Emulsions Stabilized by β-Lactoglobulin–Pectin and β-Lactoglobulin–Pectin–Chitosan Membranes Produced by the Electrostatic Layer-by-Layer Deposition Technique , 2006 .

[3]  C. Solans,et al.  Influence of the phase behavior on the properties of ionic nanoemulsions prepared by the phase inversion composition method. , 2008, Journal of colloid and interface science.

[4]  I. Norton,et al.  Oral behaviour of food hydrocolloids and emulsions. Part 2. Taste and aroma release , 2003 .

[5]  S. Talegaonkar,et al.  Design and Development of Oral Oil-in-Water Nanoemulsion Formulation Bearing Atorvastatin: In Vitro Assessment , 2010 .

[6]  M. A. Rao,et al.  The rheology of colloidal and noncolloidal food dispersions. , 2007, Journal of food science.

[7]  J. Sjöblom,et al.  Surfactants Used in Food Industry: A Review , 2009 .

[8]  F. Caruso,et al.  Biodegradable click capsules with engineered drug-loaded multilayers. , 2010, ACS nano.

[9]  J. Israelachvili Intermolecular and surface forces , 1985 .

[10]  T. Vliet,et al.  Interfacial rheological properties of adsorbed protein layers and surfactants: a review. , 2001, Advances in colloid and interface science.

[11]  P. Bummer,et al.  Physical chemical considerations of lipid-based oral drug delivery--solid lipid nanoparticles. , 2004, Critical reviews in therapeutic drug carrier systems.

[12]  T. Mason,et al.  Irreversible shear-induced vitrification of droplets into elastic nanoemulsions by extreme rupturing. , 2007, Physical review. E, Statistical, nonlinear, and soft matter physics.

[13]  D. Mcclements,et al.  Effect of interfacial protein cross-linking on the in vitro digestibility of emulsified corn oil by pancreatic lipase. , 2008, Journal of agricultural and food chemistry.

[14]  P. Given Encapsulation of Flavors in Emulsions for Beverages , 2009 .

[15]  Mischa Bonn,et al.  Label-free imaging of lipophilic bioactive molecules during lipid digestion by multiplex coherent anti-Stokes Raman scattering microspectroscopy. , 2010, Journal of the American Chemical Society.

[16]  D. Mcclements,et al.  Influence of Ostwald Ripening on Rheology of Oil-in-Water Emulsions Containing Electrostatically Stabilized Droplets , 2000 .

[17]  D. Mcclements,et al.  Color changes in hydrocarbon oil-in-water emulsions caused by Ostwald ripening. , 2001, Journal of agricultural and food chemistry.

[18]  M. Augustin,et al.  Characterization of fish oil-in-water emulsions using light-scattering, nuclear magnetic resonance, and gas chromatography-headspace analyses , 2005 .

[19]  Pieter Walstra,et al.  Principles of emulsion formation , 1993 .

[20]  P. Sanguansri,et al.  Impact of oil type on nanoemulsion formation and Ostwald ripening stability. , 2008, Langmuir : the ACS journal of surfaces and colloids.

[21]  Hans P Merkle,et al.  Microencapsulation by solvent extraction/evaporation: reviewing the state of the art of microsphere preparation process technology. , 2005, Journal of controlled release : official journal of the Controlled Release Society.

[22]  E. Dickinson Hydrocolloids at interfaces and the influence on the properties of dispersed systems , 2003 .

[23]  Cornelia M Keck,et al.  Challenges and solutions for the delivery of biotech drugs--a review of drug nanocrystal technology and lipid nanoparticles. , 2004, Journal of biotechnology.

[24]  T. Tadros,et al.  Formation and stability of nano-emulsions. , 2004, Advances in colloid and interface science.

[25]  M. Nakajima,et al.  Effect of polyglycerol esters of fatty acids on physicochemical properties and stability of β-carotene nanodispersions prepared by emulsification/evaporation method , 2005 .

[26]  J. M. Gutiérrez,et al.  Nano-emulsions: New applications and optimization of their preparation , 2008 .

[27]  S. Onoue,et al.  Physicochemical and pharmacokinetic characterization of water-soluble Coenzyme Q(10) formulations. , 2008, International journal of pharmaceutics.

[28]  M. Ashokkumar,et al.  Minimising oil droplet size using ultrasonic emulsification. , 2009, Ultrasonics sonochemistry.

[29]  A. Narang,et al.  Stable drug encapsulation in micelles and microemulsions. , 2007, International journal of pharmaceutics.

[30]  M. Nakajima,et al.  Performance of selected emulsifiers and their combinations in the preparation of β-carotene nanodispersions , 2009 .

[31]  Qingrong Huang,et al.  Bioavailability and delivery of nutraceuticals using nanotechnology. , 2010, Journal of food science.

[32]  D. Mcclements,et al.  Mass Transport Phenomena in Oil-in-Water Emulsions Containing Surfactant Micelles: Ostwald Ripening , 2000 .

[33]  E. Dickinson An introduction to food colloids , 1992 .

[34]  D. Mcclements,et al.  Formation, stability and properties of multilayer emulsions for application in the food industry. , 2006, Advances in colloid and interface science.

[35]  K. Miyajima,et al.  Physical States of Surface and Core Lipids in Lipid Emulsions and Apolipoprotein Binding to the Emulsion Surface* , 1996, The Journal of Biological Chemistry.

[36]  Helmar Schubert,et al.  Product engineering of dispersed systems , 2003 .

[37]  R. Müller,et al.  Solid lipid nanoparticles for parenteral drug delivery. , 2004, Advanced drug delivery reviews.

[38]  F. Caruso,et al.  Polyelectrolyte Blend Multilayers: A Versatile Route to Engineering Interfaces and Films , 2008 .

[39]  Jae-Kwan Hwang,et al.  Effects of Surfactants on the Formation and Stability of Capsaicinloaded Nanoemulsions , 2009 .

[40]  Filippos Kesisoglou,et al.  Application of Nanoparticles in Oral Delivery of Immediate Release Formulations , 2007 .

[41]  Christy L. Cooper,et al.  Polyelectrolyte-protein complexes , 2005 .

[42]  D. Mcclements,et al.  Factors that affect the rate of oil exchange between oil-in-water emulsion droplets stabilized by a nonionic surfactant: droplet size, surfactant concentration, and ionic strength , 1993 .

[43]  J. Aubry,et al.  Dynamic emulsification and catastrophic phase inversion of lecithin-based emulsions , 2008 .

[44]  M. Ashokkumar,et al.  The use of ultrasonics for nanoemulsion preparation , 2008 .

[45]  D. Mcclements,et al.  Emulsion-based delivery systems for tributyrin, a potential colon cancer preventative agent. , 2009, Journal of agricultural and food chemistry.

[46]  K. Westesen,et al.  Novel lipid-based colloidal dispersions as potential drug administration systems – expectations and reality , 2000 .

[47]  T. Ohshima,et al.  Effects of droplet size on the oxidative stability of oil-in-water emulsions , 2005, Lipids.

[48]  L. M. Popplewell,et al.  Inhibition of citral degradation in model beverage emulsions using micelles and reverse micelles , 2010 .

[49]  A. Kabalnov Ostwald Ripening and Related Phenomena , 2001 .

[50]  J. Benoit,et al.  Design and production of nanoparticles formulated from nano-emulsion templates-a review. , 2008, Journal of controlled release : official journal of the Controlled Release Society.

[51]  C. O’Driscoll Lipid-based formulations for intestinal lymphatic delivery. , 2002, European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences.

[52]  D. Mcclements,et al.  Solubilization Kinetics of Triacyl Glycerol and Hydrocarbon Emulsion Droplets in a Micellar Solution , 1996 .

[53]  Frank Caruso,et al.  Layer-by-layer engineered capsules and their applications , 2006 .

[54]  D. Mcclements,et al.  Citral stability in oil-in-water emulsions with solid or liquid octadecane. , 2010, Journal of agricultural and food chemistry.

[55]  M. Nakajima,et al.  β-Carotene nanodispersions: preparation, characterization and stability evaluation , 2005 .

[56]  E. Dickinson Enzymic crosslinking as a tool for food colloid rheology control and interfacial stabilization , 1997 .

[57]  Bhupinder Singh,et al.  Self-emulsifying drug delivery systems (SEDDS): formulation development, characterization, and applications. , 2009, Critical reviews in therapeutic drug carrier systems.

[58]  D. Mcclements,et al.  Stabilization of phase inversion temperature nanoemulsions by surfactant displacement. , 2010, Journal of agricultural and food chemistry.

[59]  J. Ulrich,et al.  High‐Pressure Homogenization as a Process for Emulsion Formation , 2004 .

[60]  C. Tan,et al.  Optimization of processing parameters for the preparation of phytosterol microemulsions by the solvent displacement method. , 2009, Journal of agricultural and food chemistry.

[61]  T. Kousksou,et al.  Experimental determination of the nucleation probability in emulsions , 2007 .

[62]  D. Mcclements,et al.  Lipid Oxidation in Oil‐in‐Water Emulsions: Impact of Molecular Environment on Chemical Reactions in Heterogeneous Food Systems , 2000 .

[63]  J. Rieger,et al.  Organic Nanoparticles in the Aqueous Phase-Theory, Experiment, and Use. , 2001, Angewandte Chemie.

[64]  Yves-Jacques Schneider,et al.  Nanoparticles as potential oral delivery systems of proteins and vaccines: a mechanistic approach. , 2006, Journal of controlled release : official journal of the Controlled Release Society.

[65]  Thomas D. Dziubla,et al.  PEGylation of nanocarrier drug delivery systems: State of the art , 2008 .

[66]  Clarence A. Miller Spontaneous emulsification produced by diffusion. A review , 1988 .

[67]  R. Avena-Bustillos,et al.  Nanoemulsions prepared by a low-energy emulsification method applied to edible films. , 2010, Journal of agricultural and food chemistry.

[68]  E. Perrier,et al.  Nano-emulsion formulation using spontaneous emulsification: solvent, oil and surfactant optimisation. , 2004, International journal of pharmaceutics.

[69]  R. Müller,et al.  Solid lipid nanoparticles (SLN) for controlled drug delivery - a review of the state of the art. , 2000, European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V.

[70]  C. Pouton,et al.  Lipid formulations for oral administration of drugs: non-emulsifying, self-emulsifying and 'self-microemulsifying' drug delivery systems. , 2000, European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences.

[71]  E. D. Shchukin,et al.  Ostwald ripening theory: applications to fluorocarbon emulsion stability , 1992 .

[72]  H. Schubert,et al.  Product and Formulation Engineering of Emulsions , 2004 .

[73]  D. Mcclements,et al.  Physicochemical Properties of Whey Protein‐Stabilized Emulsions as affected by Heating and Ionic Strength , 1997 .

[74]  David Julian McClements,et al.  Design of nano-laminated coatings to control bioavailability of lipophilic food components. , 2010, Journal of food science.

[75]  E. Pelan,et al.  Colloidal delivery systems for micronutrients and nutraceuticals , 2008 .

[76]  K. Morishita,et al.  Emulsification of coenzyme Q10 using gum arabic increases bioavailability in rats and human and improves food-processing suitability. , 2010, Journal of nutritional science and vitaminology.

[77]  D. Mcclements,et al.  Solid Lipid Nanoparticles as Delivery Systems for Bioactive Food Components , 2008 .

[78]  E. D. Shchukin,et al.  Ostwald ripening in two-component disperse phase systems: Application to emulsion stability , 1987 .

[79]  C. Gourdon,et al.  Emulsification by ultrasound: drop size distribution and stability. , 1999, Ultrasonics sonochemistry.

[80]  Fumiyoshi Yamashita,et al.  Lipid carrier systems for targeted drug and gene delivery. , 2005, Chemical & pharmaceutical bulletin.

[81]  R. Kraenkel,et al.  Whitham method for the Benjamin-Ono-Burgers equation and dispersive shocks. , 2007, Physical review. E, Statistical, nonlinear, and soft matter physics.

[82]  M. Nakajima,et al.  Preparation and Characterization of β-Carotene Nanodispersions Prepared by Solvent Displacement Technique , 2007 .

[83]  D. Mcclements,et al.  Bio-mimetic approach to improving emulsion stability: Cross-linking adsorbed beet pectin layers using laccase , 2008 .

[84]  Duoxia Xu,et al.  Effects of Homogenization Models and Emulsifiers on the Physicochemical Properties of β-Carotene Nanoemulsions , 2010 .

[85]  L. M. Popplewell,et al.  Stability of citral in oil-in-water emulsions prepared with medium-chain triacylglycerols and triacetin. , 2009, Journal of agricultural and food chemistry.

[86]  Seid Mahdi Jafari,et al.  Production of sub-micron emulsions by ultrasound and microfluidization techniques , 2007 .

[87]  Chi-Tang Ho,et al.  Enhancing anti-inflammation activity of curcumin through O/W nanoemulsions. , 2008, Food chemistry.

[88]  Christopher J H Porter,et al.  Formulation of lipid-based delivery systems for oral administration: materials, methods and strategies. , 2008, Advanced drug delivery reviews.

[89]  J. Benoit,et al.  Nano-emulsions and nanocapsules by the PIT method: an investigation on the role of the temperature cycling on the emulsion phase inversion. , 2007, International journal of pharmaceutics.

[90]  T. Mason,et al.  Transmission of Visible and Ultraviolet Light through Charge-Stabilized Nanoemulsions , 2008 .

[91]  B. Cabane,et al.  Phase transition pathways for the production of 100 nm oil-in-water emulsions. , 2009, Physical chemistry chemical physics : PCCP.

[92]  R. Müller,et al.  Solid lipid nanoparticles (SLN) and nanostructured lipid carriers (NLC) in cosmetic and dermatological preparations. , 2002, Advanced drug delivery reviews.

[93]  A. Kühnle,et al.  Nano-emulsion formation by emulsion phase inversion , 2004 .

[94]  B. Bhandari,et al.  Nano-Emulsion Production by Sonication and Microfluidization—A Comparison , 2006 .

[95]  J. Hamilton,et al.  Incorporation of medium chain triacylglycerols into phospholipid bilayers: effect of long chain triacylglycerols, cholesterol, and cholesteryl esters. , 1996, Journal of lipid research.

[96]  O Sonneville-Aubrun,et al.  Nanoemulsions: a new vehicle for skincare products. , 2004, Advances in colloid and interface science.

[97]  Duoxia Xu,et al.  Effects of Small and Large Molecule Emulsifiers on the Characteristics of β-Carotene Nanoemulsions Prepared by High Pressure Homogenization , 2009 .

[98]  David Julian McClements,et al.  Food Emulsions: Principles, Practice, and Techniques , 1998 .

[99]  Y. Maa,et al.  Performance of sonication and microfluidization for liquid-liquid emulsification. , 1999, Pharmaceutical development and technology.

[100]  D. Quemada,et al.  Energy of interaction in colloids and its implications in rheological modeling. , 2002, Advances in colloid and interface science.

[101]  Pieter Walstra,et al.  Physical chemistry of foods , 2002 .

[102]  D. Mcclements,et al.  Formation of nanoemulsions stabilized by model food-grade emulsifiers using high-pressure homogenization: Factors affecting particle size , 2011 .

[103]  D. Mcclements,et al.  Physical Properties of Whey Protein Stabilized Emulsions as Related to pH and NaCl , 1997 .

[104]  D. Mcclements,et al.  Emulsion-based delivery systems for lipophilic bioactive components. , 2007, Journal of food science.

[105]  J. Katz,et al.  Nanoparticles and nanocapsules created using the Ouzo effect: spontaneous emulisification as an alternative to ultrasonic and high-shear devices. , 2005, Chemphyschem : a European journal of chemical physics and physical chemistry.

[106]  A. Müllertz,et al.  Bioavailability of probucol from lipid and surfactant based formulations in minipigs: influence of droplet size and dietary state. , 2008, European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V.

[107]  N. Anton,et al.  The universality of low-energy nano-emulsification. , 2009, International journal of pharmaceutics.

[108]  I. Capek Degradation of kinetically-stable o/w emulsions. , 2004, Advances in colloid and interface science.

[109]  D. Mcclements,et al.  Comparison of Gum Arabic, Modified Starch, and Whey Protein Isolate as Emulsifiers: Influence of pH, CaCl2 and Temperature , 2002 .

[110]  D. Turnbull,et al.  Kinetics of Crystal Nucleation in Some Normal Alkane Liquids , 1961 .

[111]  D. Mcclements,et al.  Fabrication of protein-stabilized nanoemulsions using a combined homogenization and amphiphilic solvent dissolution/evaporation approach , 2010 .

[112]  D. Julian McClements,et al.  Influence of pH and carrageenan type on properties of β-lactoglobulin stabilized oil-in-water emulsions , 2005 .

[113]  M. Nakajima,et al.  Preparation of Protein-Stabilized β-Carotene Nanodispersions by Emulsification–Evaporation Method , 2007 .

[114]  K. Mutch,et al.  Nanoemulsions prepared by a two-step low-energy process. , 2008, Langmuir : the ACS journal of surfaces and colloids.

[115]  D. Mcclements,et al.  Influence of droplet characteristics on the formation of oil-in-water emulsions stabilized by surfactant-chitosan layers. , 2005, Langmuir : the ACS journal of surfaces and colloids.

[116]  Xuefeng Li,et al.  Formation and stability of nanoemulsions with mixed ionic-nonionic surfactants. , 2009, Physical chemistry chemical physics : PCCP.