The effect of graft-bed irradiation on the healing of rat skin grafts.

This study explores the possible side effects on healing skin grafts of irradiation, commonly used intraoperatively following surgical tumor removal. The experimental model involved the delivery of a single 10-Gy dose of electron radiation to the recipient bed of a skin wound, followed by attachment of a full thickness rat skin autograft. Skin graft repair was assessed by light microscopy, immunohistochemistry, and transmission electron microscopy over a 3-week period for grafted and grafted-irradiated groups. Graft-bed irradiation reduced fibrinogen, fibrin, and fibronectin deposition in the wound. It also produced brief changes in the extent of both re-epithelialization and granulation tissue formation, and reduced the diameter of collagen fibrils in the granulation tissue. Despite these changes, the results suggest that graft-bed irradiation only delays the healing process, producing no serious clinical complications at the time points studied.

[1]  G. Dickson,et al.  Electron irradiation slows down wound repair in rat skin: a morphological investigation , 1994, The British journal of dermatology.

[2]  J. Uitto,et al.  Collagen gene expression and wound strength in normal and radiation-impaired wounds. A model of radiation-impaired wound healing. , 1993, The Journal of dermatologic surgery and oncology.

[3]  W. Sause,et al.  Surgical complications of intraoperative radiation therapy: The radiation therapy oncology group experience , 1992, Journal of surgical oncology.

[4]  R. Kirsner,et al.  Human wound fluid from acute wounds stimulates fibroblast and endothelial cell growth. , 1991, Journal of the American Academy of Dermatology.

[5]  James B. Mitchell,et al.  Transforming growth factor-β improves healing of radiation-impaired wounds , 1991 .

[6]  H. Withers,et al.  Effect of fibroblast implants on wound healing of irradiated skin: assay of wound strength and quantitative immunohistology of collagen. , 1991, Radiation research.

[7]  H. Withers,et al.  Radiation effect in mouse skin: dose fractionation and wound healing. , 1990, International journal of radiation oncology, biology, physics.

[8]  J. Purdy,et al.  Reversal of impaired wound healing in irradiated rats by platelet-derived growth factor-BB. , 1989, American journal of surgery.

[9]  B. Hilaris,et al.  Wound Complications of Adjuvant Radiation Therapy in Patients with Soft‐Tissue Sarcomas , 1989, Annals of surgery.

[10]  D. Cromack,et al.  Are complications in intraoperative radiation therapy more frequent than in conventional treatment? , 1989, Archives of surgery.

[11]  R. Clark,et al.  Cryptic chemotactic activity of fibronectin for human monocytes resides in the 120-kDa fibroblastic cell-binding fragment. , 1988, The Journal of biological chemistry.

[12]  R. Dewitty,et al.  Intraoperative Radiotherapy for Patients with Carcinoma of the Pancreas: The Howard University Hospital Experience, 1978–1986 , 1988, Annals of surgery.

[13]  D. Martin,et al.  Fibronectin enhances healing of excised wounds in rats. , 1988, Archives of dermatology.

[14]  H. Hoekstra,et al.  Surgical approaches and techniques in intraoperative radiotherapy for intra-abdominal, retroperitoneal, and pelvic neoplasms. , 1988, Surgery.

[15]  H. Dvorak,et al.  Fibrin containing gels induce angiogenesis. Implications for tumor stroma generation and wound healing. , 1987, Laboratory investigation; a journal of technical methods and pathology.

[16]  M. Singer,et al.  Intraoperative radiation therapy for advanced or recurrent head and neck cancer. , 1987, International journal of radiation oncology, biology, physics.

[17]  Richard O. Hynes,et al.  Integrins: A family of cell surface receptors , 1987, Cell.

[18]  H. Hoekstra,et al.  Pathological Tissue Changes Following Intraoperative Radiotherapy , 1986, American journal of clinical oncology.

[19]  J. Tepper,et al.  Complications of intraoperative radiation therapy. , 1984, International journal of radiation oncology, biology, physics.

[20]  M. Ueda,et al.  Effects of irradiation on grafted skin. , 1983, Journal of oral and maxillofacial surgery : official journal of the American Association of Oral and Maxillofacial Surgeons.

[21]  J. Tepper,et al.  Tolerance of canine anastomoses to intraoperative radiation therapy. , 1983, International journal of radiation oncology, biology, physics.

[22]  P. Hsieh,et al.  Behavior of cells seeded in isolated fibronectin matrices , 1983, The Journal of cell biology.

[23]  M. Abe,et al.  Intraoperative radiotherapy: the Japanese experience. , 1981, International journal of radiation oncology, biology, physics.

[24]  J. Ogura,et al.  Radiation therapy and wound healing delay. Animals and man. , 1967, Radiology.

[25]  H. Grillo.  Origin of fibroblasts in wound healing. An autoradiographic study of inhibition of cellular proliferation by local x-irradiation. , 1963, Annals of surgery.

[26]  D. Cromack,et al.  Acceleration of tissue repair by transforming growth factor beta 1: identification of in vivo mechanism of action with radiotherapy-induced specific healing deficits. , 1993, Surgery.