Influence of Pulsed He–Ne Laser Irradiation on the Red Blood Cell Interaction Studied by Optical Tweezers

Optical Tweezers (OT), as a revolutionary innovation in laser physics, has been extremely useful in studying cell interaction dynamics at a single-cell level. The reversible aggregation process of red blood cells (RBCs) has an important influence on blood rheological properties, but the underlying mechanism has not been fully understood. The regulating effects of low-level laser irradiation on blood rheological properties have been reported. However, the influence of pulsed laser irradiation, and the origin of laser irradiation effects on the interaction between RBCs remain unclear. In this study, RBC interaction was assessed in detail with OT. The effects of both continuous and pulsed low-level He–Ne laser irradiation on RBC aggregation was investigated within a short irradiation period (up to 300 s). The results indicate stronger intercellular interaction between RBCs in the enforced disaggregation process, and both the cell contact time and the initial contact area between two RBCs showed an impact on the measured disaggregation force. Meanwhile, the RBC aggregation force that was independent to measurement conditions decreased after a short time of pulsed He–Ne laser irradiation. These results provide new insights into the understanding of the RBC interaction mechanism and laser irradiation effects on blood properties.

[1]  J. Sixma,et al.  The mechanism of red cell (dis)aggregation investigated by means of direct cell manipulation using multiple optical trapping , 1997, British journal of haematology.

[2]  Adriana Fontes,et al.  Measuring electrical and mechanical properties of red blood cells with double optical tweezers. , 2008, Journal of biomedical optics.

[3]  A. Pribush,et al.  The mechanism of the dextran-induced red blood cell aggregation , 2007, European Biophysics Journal.

[4]  M. Haritou,et al.  Antagonistic effects of epinephrine and helium-neon (He-Ne) laser irradiation on red blood cells deformability , 1994 .

[5]  K. Sung,et al.  Theoretical and experimental studies on cross-bridge migration during cell disaggregation. , 1989, Biophysical journal.

[6]  Herbert J Meiselman,et al.  Red blood cell aggregation: 45 years being curious. , 2009, Biorheology.

[7]  Kisung Lee,et al.  Assessment of the “cross-bridge”-induced interaction of red blood cells by optical trapping combined with microfluidics , 2017, Journal of biomedical optics.

[8]  O. Baskurt,et al.  Comparison of three instruments for measuring red blood cell aggregation. , 2009, Clinical hemorheology and microcirculation.

[9]  U. Windberger,et al.  Whole Blood Viscosity, Plasma Viscosity and Erythrocyte Aggregation in Nine Mammalian Species: Reference Values and Comparison of Data , 2003, Experimental physiology.

[10]  N. M. Ahmed,et al.  Erythrocyte sedimentation rate of human blood exposed to low-level laser , 2016, Lasers in Medical Science.

[11]  A. Ashkin.  Acceleration and trapping of particles by radiation pressure , 1970 .

[12]  C. Lim,et al.  Mechanics of the human red blood cell deformed by optical tweezers , 2003 .

[13]  Bor-Wen Yang,et al.  Measuring micro-interactions between coagulating red blood cells using optical tweezers , 2010, Biomedical optics express.

[14]  Igor Meglinski,et al.  Mutual interaction of red blood cells influenced by nanoparticles , 2019, Scientific Reports.

[15]  Herbert J Meiselman,et al.  Depletion-mediated red blood cell aggregation in polymer solutions. , 2002, Biophysical journal.

[16]  J. Y. Chen,et al.  A comparative study of 632.8 and 532 nm laser irradiation on some rheological factors in human blood in vitro. , 2004, Journal of photochemistry and photobiology. B, Biology.

[17]  Byron F. Brehm-Stecher,et al.  Single-Cell Microbiology: Tools, Technologies, and Applications , 2004, Microbiology and Molecular Biology Reviews.

[18]  Shu Chien,et al.  Role of Surface Electric Charge in Red Blood Cell Interactions , 1973, The Journal of general physiology.

[19]  Igor Meglinski,et al.  Influence of interaction time on the red blood cell (dis)aggregation dynamics in vitro studied by optical tweezers , 2019, European Conference on Biomedical Optics.

[20]  A. Ashkin.  Forces of a single-beam gradient laser trap on a dielectric sphere in the ray optics regime. , 1992, Methods in cell biology.

[21]  Matti Kinnunen,et al.  Optical tweezers study of red blood cell aggregation and disaggregation in plasma and protein solutions , 2016, Journal of biomedical optics.

[22]  Ji-yao Chen,et al.  In vitro effects of helium-neon laser irradiation on human blood: blood viscosity and deformability of erythrocytes. , 2004, Photomedicine and laser surgery.

[23]  J. Weisel,et al.  Role of red blood cells in haemostasis and thrombosis , 2017, ISBT science series.

[24]  Maria D Khokhlova,et al.  Normal and system lupus erythematosus red blood cell interactions studied by double trap optical tweezers: direct measurements of aggregation forces. , 2012, Journal of biomedical optics.

[25]  Igor Meglinski,et al.  Mutual interaction of red blood cells assessed by optical tweezers and scanning electron microscopy imaging. , 2018, Optics letters.

[26]  K. Lee,et al.  Characterization at the individual cell level and in whole blood samples of shear stress preventing red blood cells aggregation. , 2016, Journal of biomechanics.

[27]  O. Baskurt,et al.  Erythrocyte aggregation: basic aspects and clinical importance. , 2013, Clinical hemorheology and microcirculation.

[28]  Halina Rubinsztein-Dunlop,et al.  Physics of optical tweezers. , 2007, Methods in cell biology.

[29]  Alexander V. Priezzhev,et al.  Measurement of interaction forces between red blood cells in aggregates by optical tweezers , 2012 .

[30]  A. Ashkin,et al.  Optical trapping and manipulation of viruses and bacteria. , 1987, Science.

[31]  M. Kinnunen,et al.  Probing the Red Blood Cells Aggregating Force With Optical Tweezers , 2016, IEEE Journal of Selected Topics in Quantum Electronics.

[32]  Jian Zhu,et al.  Effect of intravascular irradiation of He-Ne laser on cerebral infarction: hemorrheology and apoptosis , 2005, Shanghai International Conference on Laser Medicine and Surgery.

[33]  D. Siposan,et al.  Effect of low-level laser radiation on some rheological factors in human blood: an in vitro study. , 2000, Journal of clinical laser medicine & surgery.

[34]  M W Rampling,et al.  Influence of cell-specific factors on red blood cell aggregation. , 2004, Biorheology.

[35]  O. Baskurt,et al.  Hemodynamic effects of red blood cell aggregation. , 2007, Indian journal of experimental biology.