Antioxidant and antiproliferative properties of a tocotrienol-rich fraction from grape seeds.

The antioxidant and antiproliferative activities of a tocotrienol-rich fraction (TRF) obtained from grape seeds were evaluated. TRF, a mixture of γ-tocopherol and α- and γ-tocotrienol, was prepared from a methanol-soluble fraction of grape seed oils by eluting with 10% ether (v/v) using silica gel chromatography. TRF had significantly higher antioxidant and antiproliferative activities compared to other fractions. TRF showed 3.5-, 40.0-, and 39.0-fold higher ABTS radical scavenging activity, inhibition of lipid peroxidation, and reducing power, respectively, compared to α-tocopherol fraction (5% diethyl ether fraction). TRF had higher antiproliferative activity against MCF7 (81%) and NCI-H460 (76%) cells at a concentration of 1.0 mg/ml. The results suggest that TRF from grape seeds has significant health-promoting effects, having excellent antioxidant and anticancer activities.

[1]  H. Thier.  Principles of Food Science. Part 1: Food Chemistry. Herausgegeben von O. R. Fennema. Marcel Dekker Inc., New York‐Basel 1976. 1. Aufl., XI, 792 S., geb. sfr. 170.— , 1976 .

[2]  T. Dinis,et al.  Action of phenolic derivatives (acetaminophen, salicylate, and 5-aminosalicylate) as inhibitors of membrane lipid peroxidation and as peroxyl radical scavengers. , 1994, Archives of biochemistry and biophysics.

[3]  J L Witztum,et al.  Beyond cholesterol. Modifications of low-density lipoprotein that increase its atherogenicity. , 1989, The New England journal of medicine.

[4]  W. Stone,et al.  Comparative effects of RRR-alpha- and RRR-gamma-tocopherol on proliferation and apoptosis in human colon cancer cell lines , 2006, BMC Cancer.

[5]  Fereidoon Shahidi,et al.  Functional Foods: Their Role in Health Promotion and Disease Prevention , 2006 .

[6]  L. Packer,et al.  Free radical recycling and intramembrane mobility in the antioxidant properties of alpha-tocopherol and alpha-tocotrienol. , 1991, Free radical biology & medicine.

[7]  R. Parker,et al.  Hypocholesterolemic activity of synthetic and natural tocotrienols. , 1992, Journal of medicinal chemistry.

[8]  Praveen M. Bahadduri,et al.  Molecular Basis of Vitamin E Action , 2003, Journal of Biological Chemistry.

[9]  E. Niki,et al.  Comparative study on the action of tocopherols and tocotrienols as antioxidant: chemical and physical effects. , 2003, Chemistry and physics of lipids.

[10]  M. B. Wahid,et al.  Tocotrienols and cancer: beyond antioxidant activity. , 2007 .

[11]  M. Oyaizu.  Studies on products of browning reaction--antioxidative activities of products of browning reaction prepared from glucosamine , 1986 .

[12]  F. Virgili,et al.  Tocotrienol‐Rich Fraction from Palm Oil and Gene Expression in Human Breast Cancer Cells , 2004, Annals of the New York Academy of Sciences.

[13]  M. Silvia Taga,et al.  Chia seeds as a source of natural lipid antioxidants , 1984 .

[14]  K. Lee,et al.  Vitamin C equivalent antioxidant capacity (VCEAC) of phenolic phytochemicals. , 2002, Journal of agricultural and food chemistry.

[15]  C. Rice-Evans,et al.  Functional food science and defence against reactive oxidative species , 1998, British Journal of Nutrition.

[16]  Y. Basiron,et al.  Influence of palm oil or its tocotrienol-rich fraction on the lipid peroxidation potential of rat liver mitochondria and microsomes. , 1993, Biochemistry and molecular biology international.

[17]  A. Qureshi,et al.  The structure of an inhibitor of cholesterol biosynthesis isolated from barley. , 1986, The Journal of biological chemistry.

[18]  K. Adeli,et al.  Tocotrienol: a review of its therapeutic potential. , 1999, Clinical biochemistry.

[19]  William M. Breene,et al.  Some compositional properties of camelina (camelina sativa L. Crantz) seeds and oils , 1995 .

[20]  L. Packer,et al.  Isolation and identification of novel tocotrienols from rice bran with hypocholesterolemic, antioxidant, and antitumor properties. , 2000, Journal of agricultural and food chemistry.

[21]  Junsoo Lee,et al.  Antioxidant activity of methanolic extracts from some grains consumed in Korea , 2007 .

[22]  B. Halliwell,et al.  Oxygen toxicity, oxygen radicals, transition metals and disease. , 1984, The Biochemical journal.

[23]  C. Crews,et al.  Quantitation of the main constituents of some authentic grape-seed oils of different origin. , 2006, Journal of agricultural and food chemistry.

[24]  K. Nakagawa,et al.  Anti-angiogenic Potential of Tocotrienolin vitro , 2004, Biochemistry (Moscow).

[25]  C. Rice-Evans,et al.  Antioxidant activity applying an improved ABTS radical cation decolorization assay. , 1999, Free radical biology & medicine.

[26]  P. Darbre,et al.  Tocotrienols inhibit the growth of human breast cancer cells irrespective of estrogen receptor status , 1998, Lipids.

[27]  T. Mosmann.  Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. , 1983, Journal of immunological methods.

[28]  Frank B. Hu,et al.  Dietary pattern analysis: a new direction in nutritional epidemiology , 2002, Current opinion in lipidology.

[29]  Z. Beg,et al.  Hypolipidemic and antioxidant properties of tocotrienol rich fraction isolated from rice bran oil in experimentally induced hyperlipidemic rats. , 2005, Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association.

[30]  T. Yoshikawa,et al.  Tumor suppressive effects of tocotrienol in vivo and in vitro. , 2005, Cancer letters.

[31]  P. Rahimi-Moghaddam,et al.  Free-radical scavenging capacity and antioxidant activity of selected plant species from the Canadian prairies , 2004 .