Micropropagation of Different Carob (Ceratonia siliqua L.) Genotypes by Classical and New Generation Tissue Culture Techniques

In this study, micropropagation studies were carried out in comparative Plantform bioreactor system and solid media with three different carob genotypes which are growing naturally in the Mediterranean Region. First of all, micropropagation of carob genotyps were evaluated in solid MS, 1⁄2 MS and WPM media supplemented with BA (0, 0.5, 1.0, 1.5, 2.0 mg/L) and GA3 (0.1, 0.5 mg/L) for micropropagation. Based on the solid media, the best results in all three genotypes were obtained from MS and 1⁄2 MS medium containing 0.5 and 2 mg/L BA+0.5 mg/L GA3 plant growth regulatory concentrations for micropropagation. Micropropagation studies were carried out in the Plantform system with the best-defined media content. As a result of studies, Plantform system showed better plant growth, multiplication coefficient and plant quality than solid culture system in all three genotypes propagation medium. Genetic stability of plants grown in solid culture and Plantform systems was tested by SSR markers.

[1]  Sugiharto,et al.  Production of biomass and flavonoid of Gynura procumbens (Lour.) Merr shoots culture in temporary immersion system , 2018, Journal, genetic engineering & biotechnology.

[2]  J. Silva,et al.  In vitro propagation of Gerbera jamesonii Bolus ex Hooker f. in a temporary immersion bioreactor , 2017, Plant Cell, Tissue and Organ Culture (PCTOC).

[3]  V. Cavallaro,et al.  Improving in vitro mass proliferation of carob (Ceratonia siliqua L.) from seedling apices by temporary immersion systems , 2017 .

[4]  Jishuang Chen,et al.  Micropropagation of Pinellia ternata (Thunb.) Berit. Plantlets Using Temporary Immersion Bioreactors , 2017 .

[5]  J. A. Teixeira da Silva,et al.  In vitro propagation of Gerbera jamesonii Bolus ex Hooker f. in a temporary immersion bioreactor , 2017 .

[6]  P. Poonnoy,et al.  Effects of Explants Division by Cutting, Concentrations of TDZ and Number of Sub-culture Cycles on Propagation of ‘Kluai Hom Thong’ Banana in a Temporary Immersion Bioreactor System , 2017 .

[7]  Luis Gonzaga Guti errez,et al.  Micropropagation of Guadua angustifolia Kunth (Poaceae) using a temporary immersion system RITA , 2016 .

[8]  L. Iglesias-Andreu,et al.  Evaluation of different temporary immersion systems (BIT®, BIG, and RITA®) in the micropropagation of Vanilla planifolia Jacks , 2016, In Vitro Cellular & Developmental Biology - Plant.

[9]  Jiang Wen,et al.  Optimization of Sagittaria sagittifolia Rapid Propagation in Temporary Immersion Bioreactors System , 2016 .

[10]  R. Repin,et al.  Micropropagation of an endangered Borneo Orchid, Paphiopedilum rothschildianum Callus using Temporary Immersion Bioreactor System , 2016 .

[11]  B. Ruffoni,et al.  EVALUATION OF TEMPORARY IMMERSION SYSTEM FOR IN VITRO PROPAGATION OF STEVIA REBAUDIANA BERTONI , 2015 .

[12]  T. Liwang,et al.  Micropropagation of Embryogenic Callus of Oil Palm (Elaeis Guineensis Jacq.) Using Temporary Immersion System , 2015 .

[13]  E. Sgarbi,et al.  Comparison between a conventional culture system and Plantform bioreactor in Quercus robur micropropagation , 2015 .

[14]  Li-Hua Zhu,et al.  Evaluation of a new vessel system based on temporary immersion system for micropropagation , 2014 .

[15]  S. L. Malfa,et al.  Genetic diversity revealed by EST-SSR markers in carob tree (Ceratonia siliqua L.) , 2014 .

[16]  E. Déchamp,et al.  Bioreactors in coffee micropropagation , 2006 .

[17]  H. Etienne,et al.  Temporary immersion systems in plant micropropagation , 2002, Plant Cell, Tissue and Organ Culture.

[18]  S. Takayama,et al.  Mass Propagation of Begonia × hiemalis Plantlets by Shake Culture , 1981 .