Utilization of laser‐assisted analytical methods for monitoring of lead and nutrition elements distribution in fresh and dried Capsicum annuum l. leaves

Laser induced breakdown spectroscopy (LIBS) and laser ablation inductively coupled plasma mass spectrometry (LA‐ICP‐MS) have been applied for high‐resolution mapping of accumulation and distribution of heavy metal (lead) and nutrition elements (potassium, manganese) in leaves of Capsicum annuum L. samples. Lead was added in a form of Pb(NO3)2 at concentration up to 10 mmol L−1 into the vessels that contained tap water and where the 2‐months old Capsicum annuum L. plants were grown another seven days. Two dimensional maps of the elements are presented for both laser‐assisted analytical methods. Elemental mapping performed on fresh (frozen) and dried Capsicum annuum L. leaves are compared. Microsc. Res. Tech., 2011. © 2010 Wiley‐Liss, Inc.

[1]  Donghua Liu,et al.  Uptake and accumulation of lead by roots, hypocotyls and shoots of Indian mustard (Brassica juncea (L.)). , 2000 .

[2]  W. Stahel,et al.  Log-normal Distributions across the Sciences: Keys and Clues , 2001 .

[3]  Walter W. Wenzel,et al.  Role of assisted natural remediation in environmental cleanup , 2004 .

[4]  A. Elias,et al.  Metal associations in soils before and after EDTA extractive decontamination: implications for the effectiveness of further clean-up procedures. , 2001, Environmental pollution.

[5]  E. Olguín,et al.  Leaching of lead by ammonium salts and EDTA from Salvinia minima biomass produced during aquatic phytoremediation. , 2008, Journal of hazardous materials.

[6]  A. Matusch,et al.  Scaling down the bioimaging of metals by laser microdissection inductively coupled plasma mass spectrometry (LMD-ICP-MS) , 2010 .

[7]  P. Filzmoser,et al.  Normal and lognormal data distribution in geochemistry: death of a myth. Consequences for the statistical treatment of geochemical and environmental data , 2000 .

[8]  V. Adam,et al.  Investigation of heavy-metal accumulation in selected plant samples using laser induced breakdown spectroscopy and laser ablation inductively coupled plasma mass spectrometry , 2008 .

[9]  Y. Zuily-Fodil,et al.  Accumulation of lead in the roots of grass pea (Lathyrus sativus L.) plants triggers systemic variation in gene expression in the shoots. , 2009, Chemosphere.

[10]  S. Verma,et al.  Lead toxicity induces lipid peroxidation and alters the activities of antioxidant enzymes in growing rice plants , 2003 .

[11]  D. Leung,et al.  Chelated lead transport in Pinus radiata: an ultrastructural study , 2002 .

[12]  M. Del Bubba,et al.  Heavy metal distribution between contaminated soil and Paulownia tomentosa, in a pilot-scale assisted phytoremediation study: influence of different complexing agents. , 2008, Chemosphere.

[13]  Francesco Flora,et al.  Mapping of the metal intake in plants by large-field X-ray microradiography and preliminary feasibility studies in microtomography , 2005 .

[14]  Lucia Reale,et al.  Mapping of lead, magnesium and copper accumulation in plant tissues by laser-induced breakdown spectroscopy and laser-ablation inductively coupled plasma mass spectrometry , 2009 .

[15]  Jianguo Liu,et al.  Accumulation of Cd, Pb and Zn by 19 wetland plant species in constructed wetland. , 2007, Journal of hazardous materials.

[16]  Ota Samek,et al.  Sensitive and selective spectrochemical analysis of metallic samples: the combination of laser-induced breakdown spectroscopy and laser-induced fluorescence spectroscopy , 2001 .

[17]  N. Zhang,et al.  Determination of Cd, Co, Ni and Pb in biological samples by microcolumn packed with black stone (Pierre noire) online coupled with ICP-OES. , 2008, Journal of hazardous materials.

[18]  T. Cutright,et al.  Hydroponic phytoremediation of Cd, Cr, Ni, As, and Fe: can Helianthus annuus hyperaccumulate multiple heavy metals? , 2008, Chemosphere.

[19]  J. Oehlmann,et al.  Biomonitoring of metal contamination in a marine prosobranch snail (Nassarius reticulatus) by imaging laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). , 2009, Talanta.

[20]  Catherine N. Mulligan,et al.  Remediation technologies for metal-contaminated soils and groundwater: an evaluation , 2001 .

[21]  M. Arruda,et al.  Determination of cadmium and lead at low levels by using preconcentration at fullerene coupled to thermospray flame furnace atomic absorption spectrometry , 2004 .

[22]  Pavel Tlustos,et al.  The use of maize and poplar in chelant-enhanced phytoextraction of lead from contaminated agricultural soils. , 2007, Chemosphere.

[23]  F. Flora,et al.  Monitoring of the heavy‐metal hyperaccumulation in vegetal tissues by X‐ray radiography and by femto‐second laser induced breakdown spectroscopy , 2007, Microscopy research and technique.

[24]  Hua Li,et al.  EDTA-enhanced phytoremediation of lead contaminated soil by Bidens maximowicziana. , 2007, Journal of environmental sciences.

[25]  P. Römkens,et al.  Potentials and drawbacks of chelate-enhanced phytoremediation of soils. , 2002, Environmental pollution.

[26]  M. Kirkham,et al.  Heavy metal displacement in salt-water-irrigated soil during phytoremediation. , 2008, Environmental pollution.

[27]  D. Barałkiewicz,et al.  Enhancing phytoremediative ability of Pisum sativum by EDTA application. , 2003, Phytochemistry.

[28]  D. Antosiewicz Study of calcium-dependent lead-tolerance on plants differing in their level of Ca-deficiency tolerance. , 2005, Environmental pollution.

[29]  Lucia Reale,et al.  Utilization of laser induced breakdown spectroscopy for investigation of the metal accumulation in vegetal tissues , 2007 .

[30]  N. Terryn,et al.  Lead accumulation in the roots of grass pea (Lathyrus sativus L.): a novel plant for phytoremediation systems? , 2008, Comptes rendus biologies.

[31]  Karel Novotný,et al.  Implementation of an autofocus algorithm based on searching the best in-focus image into a table-top laser-induced breakdown spectroscopy setup , 2009 .

[32]  Karel Novotný,et al.  Mapping of different structures on large area of granite sample using laser-ablation based analytical techniques, an exploratory study , 2008 .

[33]  Christian L. Goueguel,et al.  Laser-induced fluorescence detection of lead atoms in a laser-induced plasma: An experimental analytical optimization study , 2009 .

[34]  H M Chen,et al.  Chemical methods and phytoremediation of soil contaminated with heavy metals. , 2000, Chemosphere.

[35]  Archana Sharma,et al.  Comparison of mercury, lead and arsenic with respect to genotoxic effects on plant systems and the development of genetic tolerance , 2004 .

[36]  P. Masson,et al.  Determination of Major and Trace Elements in Plant Samples by Inductively Coupled Plasma–Mass Spectrometry , 2010 .

[37]  C. Palm,et al.  Bioimaging of metals by laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). , 2010, Mass spectrometry reviews.

[38]  U. Krämer,et al.  Phytoremediation: novel approaches to cleaning up polluted soils. , 2005, Current opinion in biotechnology.

[39]  J. Pichtel,et al.  Distribution of Pb, Cd and Ba in soils and plants of two contaminated sites. , 2000, Environmental pollution.