Glycoprotein Structure Determination by Mass Spectrometry

The human genome encodes 30,000 to 40,000 proteins, and a major challenge is to understand how posttranslational events, such as glycosylation, affect the activities and functions of these proteins in health and disease. Glycosylated proteins are ubiquitous components of extracellular matrices and cellular surfaces where their oligosaccharide moieties are implicated in a wide range of cell-cell and cell-matrix recognition events. The power of ultrahigh-sensitivity mass spectrometric strategies for defining the primary structures of highly complex mixtures of glycoprotein glycoforms is set to revolutionize structural glycobiology in the coming postgenomic era.

[1]  A. Dell,et al.  High sensitivity collisionally-activated decomposition tandem mass spectrometry on a novel quadrupole/orthogonal-acceleration time-of-flight mass spectrometer. , 1996, Rapid communications in mass spectrometry : RCM.

[2]  K. Yamashita,et al.  Electrospray ionization-mass spectrometric analysis of serum transferrin isoforms in patients with carbohydrate-deficient glycoprotein syndrome. , 1993, Journal of biochemistry.

[3]  H. Rahmoune,et al.  Structures of monosialyl oligosaccharides isolated from the respiratory mucins of a non-secretor (O, Lea+b-) patient suffering from chronic bronchitis. Characterization of a novel type of mucin carbohydrate core structure. , 1994, Glycobiology.

[4]  A. Dell,et al.  A role for glycoconjugates in human development: the human feto-embryonic defence system hypothesis. , 1996, Human reproduction.

[5]  Gary F. Clark,et al.  Structural Analysis of the Oligosaccharides Derived from Glycodelin, a Human Glycoprotein with Potent Immunosuppressive and Contraceptive Activities (*) , 1995, The Journal of Biological Chemistry.

[6]  K. Khoo,et al.  Immunogenic glycoconjugates implicated in parasitic nematode diseases. , 1999, Biochimica et biophysica acta.

[7]  Andrew F. Hill,et al.  Molecular analysis of prion strain variation and the aetiology of 'new variant' CJD , 1996, Nature.

[8]  L. Hood,et al.  Structural studies of the scrapie prion protein using mass spectrometry and amino acid sequencing. , 1993, Biochemistry.

[9]  R. U. Margolis,et al.  High prevalence of 2-mono- and 2,6-di-substituted manol-terminating sequences among O-glycans released from brain glycopeptides by reductive alkaline hydrolysis. , 1999, European journal of biochemistry.

[10]  Mark Sutton-Smith,et al.  A rapid mass spectrometric strategy suitable for the investigation of glycan alterations in knockout mice , 2000 .

[11]  John B. Fenn,et al.  Electrospray ionization–principles and practice , 1990 .

[12]  A. Chong,et al.  Biochemical typing of scrapie strains , 1997, Nature.

[13]  S. Prusiner,et al.  Glycosylation differences between the normal and pathogenic prion protein isoforms. , 1999, Proceedings of the National Academy of Sciences of the United States of America.

[14]  J. Peter-Katalinic,et al.  High Density O-Glycosylation on Tandem Repeat Peptide from Secretory MUC1 of T47D Breast Cancer Cells* , 1999, The Journal of Biological Chemistry.

[15]  J. Vliegenthart,et al.  Isolation and structural characterization of novel sialylated oligosaccharide-alditols from respiratory-mucus glycoproteins of a patient suffering from bronchiectasis. , 1993, European journal of biochemistry.

[16]  A. Dell,et al.  Structural Analysis of Murine Zona Pellucida Glycans , 2000, The Journal of Biological Chemistry.

[17]  A. Shevchenko,et al.  MALDI quadrupole time-of-flight mass spectrometry: a powerful tool for proteomic research. , 2000, Analytical chemistry.

[18]  Gary F. Clark,et al.  Pregnancy-associated Changes in the Glycosylation of Tamm-Horsfall Glycoprotein , 2000, The Journal of Biological Chemistry.

[19]  Gary F. Clark,et al.  Gender-specific Glycosylation of Human Glycodelin Affects Its Contraceptive Activity* , 1996, The Journal of Biological Chemistry.

[20]  M. Glick,et al.  Terminal glycosylation in cystic fibrosis. , 1999, Biochimica et biophysica acta.

[21]  Trevor S. Smith,et al.  Haemonchus contortus Glycoproteins Contain N-Linked Oligosaccharides with Novel Highly Fucosylated Core Structures* , 1996, The Journal of Biological Chemistry.

[22]  K. Miyamoto,et al.  Expression of luteinizing hormone/human chorionic gonadotrophin (LH/HCG) receptor mRNA in the human ovary. , 1997, Molecular human reproduction.

[23]  K. Khoo,et al.  Structural definition of the non-reducing termini of mannose-capped LAM from Mycobacterium tuberculosis through selective enzymatic degradation and fast atom bombardment-mass spectrometry. , 1993, Glycobiology.

[24]  M. Guilhaus,et al.  Orthogonal acceleration time-of-flight mass spectrometry. , 2000, Mass spectrometry reviews.

[25]  J R Yates,et al.  Protein sequencing by tandem mass spectrometry. , 1986, Proceedings of the National Academy of Sciences of the United States of America.

[26]  R. Hermann,et al.  Human protein C inhibits selectin-mediated cell adhesion: role of unique fucosylated oligosaccharide. , 1994, Glycobiology.

[27]  G. Hansson,et al.  Sequencing of sulfated oligosaccharides from mucins by liquid chromatography and electrospray ionization tandem mass spectrometry. , 2000, Analytical chemistry.

[28]  H. Morris.  Biomolecular structure determination by mass spectrometry , 1980, Nature.

[29]  J. Marth,et al.  Genetic remodeling of protein glycosylation in vivo induces autoimmune disease. , 2001, Proceedings of the National Academy of Sciences of the United States of America.

[30]  S. Prusiner,et al.  Prion protein conformation in a patient with sporadic fatal insomnia. , 1999, The New England journal of medicine.

[31]  W. Ens,et al.  Daughter ion mass spectra from cationized molecules of small oligopeptides in a reflecting time-of-flight mass spectrometer. , 1988, Analytical chemistry.

[32]  J. Taylor‐Papadimitriou,et al.  Biology, biochemistry and immunology of carcinoma-associated mucins. , 1997, Immunology today.

[33]  M. Karas,et al.  Ultraviolet–laser desorption/ionization mass spectrometry of femtomolar amounts of large proteins , 1989 .

[34]  A. Dell,et al.  Characterization of the N-linked glycans of adult Trichinella spiralis. , 2000, Molecular and biochemical parasitology.

[35]  F. Cohen,et al.  Eight prion strains have PrPSc molecules with different conformations , 1998, Nature Medicine.

[36]  M R Thompson,et al.  Antifreeze glycoproteins from the blood of an antarctic fish. The structure of the proline-containing glycopeptides. , 1978, The Journal of biological chemistry.

[37]  A. Dell,et al.  Structural characterization of the N-glycans of Dictyocaulus viviparus: discovery of the Lewis(x) structure in a nematode. , 2000, Glycobiology.

[38]  K. Khoo,et al.  Structural mapping of the glycans from the egg glycoproteins of Schistosoma mansoni and Schistosoma japonicum: identification of novel core structures and terminal sequences. , 1997, Glycobiology.

[39]  A. Dell,et al.  Structural Studies of N-Glycans of Filarial Parasites , 1999, The Journal of Biological Chemistry.

[40]  Y. Wada,et al.  Structure of serum transferrin in carbohydrate-deficient glycoprotein syndrome. , 1992, Biochemical and biophysical research communications.

[41]  A. Burlingame,et al.  Site-specific characterization of the N-linked glycans of murine prion protein by high-performance liquid chromatography/electrospray mass spectrometry and exoglycosidase digestions. , 1999, Biochemistry.

[42]  A. Dell,et al.  F.A.B.-mass spectrometry of carbohydrates. , 1987, Advances in carbohydrate chemistry and biochemistry.

[43]  K. Biemann.  Sequencing of peptides by tandem mass spectrometry and high-energy collision-induced dissociation. , 1990, Methods in enzymology.

[44]  R. Bateman,et al.  Ionisation and fragmentation of complex glycans with a quadrupole time-of-flight mass spectrometer fitted with a matrix-assisted laser desorption/ionisation ion source. , 2000, Rapid communications in mass spectrometry : RCM.