Displacement electrophoresis of ampholytes in a continuous pH gradient moving in a capillary with a non-constant cross-section

Abstract Our previous contribution described the construction and function of miniaturized automated instrumentation for displacement electrophoresis using a separation compartment consisting of a tapered channel coupled to a constant cross-section detection fused-silica capillary. In this work, the above instrumentation is used to study the separation and focusing of the zones of ampholytes during their movement on the background of continuous pH gradient generated by carrier ampholytes. Dependencies of the resolution of chosen colored low-molecular-mass ampholytes on the working parameters - including the length of the detection fused-silica capillary, concentration of counterion in the leading electrolyte, electric current, amount of carrier ampholytes and the analyte acidobasic properties - are evaluated. A reasonable speed of separation and resolution can be achived at a potential drop over the separation compartment down to 200 V.

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