The rhodopsin gene of the cuttlefish Sepia officinalis: sequence and spectral tuning.

The cephalopod molluscs are a group of invertebrates that occupy a wide range of oceanic photic environments. They are an ideal group of animals, therefore, in which to study the evolution of rhodopsin. The cDNA sequence of the rhodopsin gene of the cuttlefish Sepia officinalis (L.) (Sub-class Coleoidea, Order Sepiida) is presented, together with an analysis of the structure of the gene. A proline-rich C terminus is present; this structure is characteristic of cephalopod rhodopsins. In common with all invertebrate opsins studied so far, the equivalent site to the counterion in vertebrate opsins is occupied by an aromatic amino acid. An intron is present that splits codon 107, in contrast to the intronless rhodopsin gene in two species of myopsid squid. A spectral tuning model involving substitutions at only three amino acid sites is proposed for the spectral shifts between the rhodopsins of Sepia officinalis, three species of squid and Paroctopus defleini.

[1]  H. Takeuchi,et al.  Ultraviolet resonance Raman evidence for the absence of tyrosinate in octopus rhodopsin and the participation of Trp residues in the transition to acid metarhodopsin , 1996, FEBS letters.

[2]  Fumio Tokunaga,et al.  Isolation and sequence determination of the chicken rhodopsin gene , 1988, Vision Research.

[3]  R. Hara,et al.  Cloning and nucleotide sequence of cDNA for rhodopsin of the squid Todarodes pacificus , 1993, FEBS letters.

[4]  S. Yokoyama,et al.  Molecular characterization of a blue visual pigment gene in the fish Astyanax fasciatus , 1993, FEBS letters.

[5]  D. Oprian,et al.  Molecular determinants of human red/green color discrimination , 1994, Neuron.

[6]  D. Oprian,et al.  Effect of carboxylic acid side chains on the absorption maximum of visual pigments. , 1989, Science.

[7]  J. Baldwin.  The probable arrangement of the helices in G protein‐coupled receptors. , 1993, The EMBO journal.

[8]  J. Messenger.  Comparative Physiology of Vision in Molluscs , 1981 .

[9]  G. Rubin,et al.  An opsin gene expressed in only one photoreceptor cell type of the Drosophila eye , 1986, Cell.

[10]  Y. Shimonishi,et al.  Identification of Two Palmitoyl Groups in Octopus Rhodopsin , 1997, Photochemistry and photobiology.

[11]  N. Ryba,et al.  Molecular cloning and primary structure of squid (Loligo forbesi) rhodopsin, a phospholipase C-directed G-protein-linked receptor. , 1991, The Biochemical journal.

[12]  H. Khorana,et al.  Orientation of retinal in bovine rhodopsin determined by cross-linking using a photoactivatable analog of 11-cis-retinal. , 1990, The Journal of biological chemistry.

[13]  J. Nathans.  Determinants of visual pigment absorbance: role of charged amino acids in the putative transmembrane segments. , 1990, Biochemistry.

[14]  Rainer Fuchs,et al.  CLUSTAL V: improved software for multiple sequence alignment , 1992, Comput. Appl. Biosci..

[15]  E. Clarkson.  Invertebrate Palaeontology and Evolution , 1979 .

[16]  W. Baehr,et al.  Isolation and analysis of the mouse opsin gene , 1988, FEBS letters.

[17]  J. Nathans,et al.  Molecular genetics of human color vision: the genes encoding blue, green, and red pigments. , 1986, Science.

[18]  J. Partridge,et al.  Mechanisms of wavelength tuning in the rod opsins of deep-sea fishes , 1997, Proceedings of the Royal Society of London. Series B: Biological Sciences.

[19]  G. Lauder.  PHYLOGENY AND PHYSIOLOGY , 1988 .

[20]  H. Khorana,et al.  Rhodopsin mutants that bind but fail to activate transducin. , 1990, Science.

[21]  H. Saibil,et al.  Effect of the C‐terminal proline repeats on ordered packing of squid rhodopsin and its mobility in membranes , 1995, FEBS letters.

[22]  J Nathans,et al.  ROLE OF HYDROXYL‐BEARING AMINO ACIDS IN DIFFERENTIALLY TUNING THE ABSORPTION SPECTRA OF THE HUMAN RED AND GREEN CONE PIGMENTS , 1993, Photochemistry and photobiology.

[23]  C. López-Larrea,et al.  Gene walking by unpredictably primed PCR. , 1994, Nucleic acids research.

[24]  D M Hunt,et al.  Characterisation of the ultraviolet-sensitive opsin gene in the honey bee, Apis mellifera. , 1997, European journal of biochemistry.

[25]  J. Bowmaker,et al.  The rhodopsin-encoding gene of bony fish lacks introns. , 1995, Gene.

[26]  G. Rubin,et al.  A second opsin gene expressed in the ultraviolet-sensitive R7 photoreceptor cells of Drosophila melanogaster , 1987, The Journal of neuroscience : the official journal of the Society for Neuroscience.

[27]  H. Khorana,et al.  Cysteine residues 110 and 187 are essential for the formation of correct structure in bovine rhodopsin. , 1988, Proceedings of the National Academy of Sciences of the United States of America.

[28]  B. Gowen,et al.  Projection structure of an invertebrate rhodopsin. , 1996, Journal of structural biology.

[29]  Y. Ovchinnikov,et al.  Octopus rhodopsin Amino acid sequence deduced from cDNA , 1988, FEBS letters.

[30]  J. Bowmaker,et al.  The molecular basis of a spectral shift in the rhodopsins of two species of squid from different photic environments , 1993, Proceedings of the Royal Society of London. Series B: Biological Sciences.

[31]  J. Nathans,et al.  Isolation and nucleotide sequence of the gene encoding human rhodopsin. , 1984, Proceedings of the National Academy of Sciences of the United States of America.

[32]  D. Hunt,et al.  Spectral tuning and molecular evolution of rod visual pigments in the species flock of cottoid fish in Lake Baikal , 1996, Vision Research.

[33]  Gebhard F. X. Schertler,et al.  Projection structure of rhodopsin , 1993, Nature.

[34]  Richard L. Martin,et al.  The Drosophila ninaE gene encodes an opsin , 1985, Cell.