Anti-HIV-1 activity of propolis in CD4(+) lymphocyte and microglial cell cultures.

An urgent need for additional agents to treat human immunodeficiency virus type 1 (HIV-1) infection led us to assess the anti-HIV-1 activity of the natural product propolis in CD4(+) lymphocytes and microglial cell cultures. Propolis inhibited viral expression in a concentration-dependent manner (maximal suppression of 85 and 98% was observed at 66.6 microg/ml propolis in CD4(+) and microglial cell cultures, respectively). Similar anti-HIV-1 activity was observed with propolis samples from several geographic regions. The mechanism of propolis antiviral property in CD4(+) lymphocytes appeared to involve, in part, inhibition of viral entry. While propolis had an additive antiviral effect on the reverse transcriptase inhibitor zidovudine, it had no noticeable effect on the protease inhibitor indinavir. The results of this in vitro study support the need for clinical trials of propolis or one or more of its components in the treatment of HIV-1 infection.

[1]  L. Farias,et al.  Brazilian Propolis: physicochemical properties, plant origin and antibacterial activity on periodontopathogens , 2003, Phytotherapy research : PTR.

[2]  J. Boustie,et al.  Comparison of the anti-herpes simplex virus activities of propolis and 3-methyl-but-2-enyl caffeate. , 1994, Journal of natural products.

[3]  H. Gendelman,et al.  Glutamate is a mediator of neurotoxicity in secretions of activated HIV-1-infected macrophages , 2001, Journal of Neuroimmunology.

[4]  C. Broder,et al.  Fusogenic mechanisms of enveloped-virus glycoproteins analyzed by a novel recombinant vaccinia virus-based assay quantitating cell fusion-dependent reporter gene activation , 1994, Journal of virology.

[5]  J. Falloon,et al.  The effect of garlic supplements on the pharmacokinetics of saquinavir. , 2002, Clinical infectious diseases : an official publication of the Infectious Diseases Society of America.

[6]  J. Falloon,et al.  Indinavir concentrations and St John's wort , 2000, The Lancet.

[7]  Yi Xia,et al.  Recent advances in the discovery and development of flavonoids and their analogues as antitumor and anti-HIV agents. , 1998, Advances in experimental medicine and biology.

[8]  A. Rubinstein,et al.  Suppression of HIV-1 replication by propolis and its immunoregulatory effect. , 1997, Drugs under experimental and clinical research.

[9]  G. Burdock,et al.  Review of the biological properties and toxicity of bee propolis (propolis). , 1998, Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association.

[10]  Shigetoshi Kadota,et al.  Recent progress in pharmacological research of propolis , 2001, Phytotherapy research : PTR.

[11]  T. Folks,et al.  Inhibition of HIV activation in latently infected cells by flavonoid compounds. , 1996, AIDS research and human retroviruses.

[12]  P. But,et al.  Inhibitory activity of flavonoids and tannins against HIV-1 protease. , 2000, Biological & pharmaceutical bulletin.

[13]  P. Peterson,et al.  Naltrexone potentiates anti-HIV-1 activity of antiretroviral drugs in CD4+ lymphocyte cultures. , 2001, Drug and alcohol dependence.

[14]  M. C. Marcucci,et al.  Propolis: recent advances in chemistry and plant origin , 2000 .

[15]  Daciana Ciocan,et al.  PLANT PRODUCTS AS ANTIMICROBIAL AGENTS , 2007 .

[16]  E. De Clercq.  Current lead natural products for the chemotherapy of human immunodeficiency virus (HIV) infection , 2000, Medicinal research reviews.

[17]  R. Krell,et al.  Value-added products from beekeeping , 1996 .

[18]  C. Broder,et al.  Consistent and significant inhibition of human immunodeficiency virus type 1 envelope-mediated membrane fusion by beta-chemokines (RANTES) in primary human macrophages. , 2000, The Journal of infectious diseases.

[19]  Current lead natural products for the chemotherapy of human immunodeficiency virus (HIV) infection , 2000 .

[20]  K. Kohn,et al.  Hydroxylated aromatic inhibitors of HIV-1 integrase. , 1995, Journal of medicinal chemistry.

[21]  P. Peterson,et al.  Kappa-opioid receptor agonist inhibition of HIV-1 envelope glycoprotein-mediated membrane fusion and CXCR4 expression on CD4(+) lymphocytes. , 2002, Biochemical pharmacology.

[22]  C. Pannecouque,et al.  Antiviral activity against human immunodeficiency virus type 1 (HIV‐1) and type 2 (HIV‐2) of ethnobotanically selected Ethiopian medicinal plants , 2001, Phytotherapy research : PTR.

[23]  F. Chang,et al.  Anti-AIDS agents. 48.(1) Anti-HIV activity of moronic acid derivatives and the new melliferone-related triterpenoid isolated from Brazilian propolis. , 2001, Journal of natural products.

[24]  P. Portoghese,et al.  Endomorphin-1 potentiates HIV-1 expression in human brain cell cultures: implication of an atypical μ-opoid receptor , 1999, Neuropharmacology.

[25]  J. Serkedjieva,et al.  Anti-influenza virus effect of some propolis constituents and their analogues (esters of substituted cinnamic acids). , 1992, Journal of natural products.

[26]  N. Vynograd,et al.  A comparative multi-centre study of the efficacy of propolis, acyclovir and placebo in the treatment of genital herpes (HSV). , 2000, Phytomedicine : international journal of phytotherapy and phytopharmacology.