On the use of photoacoustics to detect red blood cell aggregation

The feasibility of detecting red blood cell (RBC) aggregation with photoacoustics (PAs) was investigated theoretically and experimentally using human and porcine RBCs. The theoretical PA signals and spectra generated from such samples were examined for several hematocrit levels and aggregates sizes. The effect of a finite transducer bandwidth on the received PA signal was also examined. The simulation results suggest that the dominant frequency of the PA signals from non-aggregated RBCs decreases towards clinical frequency ranges as the aggregate size increases. The experimentally measured mean spectral power increased by ~6 dB for the largest aggregate compared to the non-aggregated samples. Such results confirm the theoretical predictions and illustrate the potential of using PA imaging for detecting RBC aggregation.

[1]  C. Falcó,et al.  Erythrocyte aggregation determined with the Myrenne aggregometer at two modes (M0, M1) and at two times (5 and 10 sec). , 2003, Clinical hemorheology and microcirculation.

[2]  Geng Ku,et al.  Noninvasive imaging of hemoglobin concentration and oxygenation in the rat brain using high-resolution photoacoustic tomography. , 2006, Journal of biomedical optics.

[3]  Michael C. Kolios,et al.  Photoacoustic ultrasound spectroscopy for assessing red blood cell aggregation and oxygenation , 2012, Journal of biomedical optics.

[4]  Michael C. Kolios,et al.  Effects of erythrocyte oxygenation on optoacoustic signals. , 2011, Journal of biomedical optics.

[5]  H J Meiselman,et al.  Red blood cell aggregation in experimental sepsis. , 1997, The Journal of laboratory and clinical medicine.

[6]  O. Baskurt,et al.  Blood Rheology and Hemodynamics , 2003, Seminars in thrombosis and hemostasis.

[7]  O. Baskurt,et al.  Erythrocyte aggregation tendency and cellular properties in horse, human, and rat: a comparative study. , 1997, American journal of physiology. Heart and circulatory physiology.

[8]  I. Shapira,et al.  Enhanced Erythrocyte Aggregation in Clinically Diagnosed Pelvic Inflammatory Disease , 2005, Sexually transmitted diseases.

[9]  H J Meiselman,et al.  Cellular determinants of low-shear blood viscosity. , 1997, Biorheology.

[10]  Oguz K. Baskurt,et al.  Handbook of hemorheology and hemodynamics , 2007 .

[11]  Michael C. Kolios,et al.  A simulation study on photoacoustic signals from red blood cells. , 2011, The Journal of the Acoustical Society of America.

[12]  Johannes G. G. Dobbe,et al.  Syllectometry: the effect of aggregometer geometry in the assessment of red blood cell shape recovery and aggregation , 2003, IEEE Transactions on Biomedical Engineering.

[13]  E. Feleppa,et al.  Relationship of Ultrasonic Spectral Parameters to Features of Tissue Microstructure , 1987, IEEE Transactions on Ultrasonics, Ferroelectrics and Frequency Control.

[14]  O. Baskurt,et al.  Wavelength selection in measuring red blood cell aggregation based on light transmittance. , 2011, Journal of biomedical optics.

[15]  Guy Cloutier,et al.  Ultrasonic parametric imaging of erythrocyte aggregation using the structure factor size estimator. , 2009, Biorheology.

[16]  Lihong V. Wang,et al.  Biomedical Optics: Principles and Imaging , 2007 .

[17]  Lihong V. Wang,et al.  Photoacoustic Tomography: In Vivo Imaging from Organelles to Organs , 2012, Science.

[18]  Guy Cloutier,et al.  Experimental ultrasound characterization of red blood cell aggregation using the structure factor size estimator. , 2007, The Journal of the Acoustical Society of America.

[19]  Lihong V. Wang,et al.  Prospects of photoacoustic tomography. , 2008, Medical physics.

[20]  Herbert J Meiselman,et al.  Red blood cell aggregation: 45 years being curious. , 2009, Biorheology.

[21]  M Intaglietta,et al.  Effects of red cell aggregation on the venous microcirculation. , 1999, Biorheology.

[22]  M. Plebani,et al.  Length of Sedimentation Reaction in Undiluted Blood (Erythrocyte Sedimentation Rate): Variations with Sex and Age and Reference Limits , 2001, Clinical chemistry and laboratory medicine.

[23]  Mustafa Sarimollaoglu,et al.  In vivo flow cytometry of circulating clots using negative photothermal and photoacoustic contrasts , 2011, Cytometry. Part A : the journal of the International Society for Analytical Cytology.

[24]  Einar L. Hinrichsen,et al.  Random packing of disks in two dimensions , 1990 .

[25]  Oguz K. Baskurt,et al.  Red Blood Cell Aggregation , 2011 .

[26]  Michael C. Kolios,et al.  Validity of a theoretical model to examine blood oxygenation dependent optoacoustics. , 2012, Journal of biomedical optics.

[27]  Srivalleesha Mallidi,et al.  Combined ultrasound and photoacoustic imaging to detect and stage deep vein thrombosis: phantom and ex vivo studies. , 2008, Journal of biomedical optics.

[28]  R. Cobbold.  Foundations of Biomedical Ultrasound , 2006 .

[29]  Gerald J. Diebold,et al.  1 Photoacoustic Monopole Radiation: Waves from Objects with Symmetry in One, Two, and Three Dimensions , 2009 .

[30]  Vladimir P Zharov,et al.  Photoacoustic flow cytometry. , 2012, Methods.