Effects of ice structuring proteins on freeze-thaw stability of corn and wheat starch gels.

ABSTRACT The effect of ice structuring proteins (ISP, 0.5%, starch weight basis) on the freeze-thaw stability of corn and wheat starch gels (CS and WS, respectively) was studied. Syneresis measurement, hardness, thermal properties using differential scanning calorimetry (DSC), X-ray diffractions, and scanning electron microscopy (SEM) were determined with starch gels (SG) subjected up to nine freeze-thaw cycles (FTC). The gelatinized SG were stored at –20°C for 22 hr and thawed at 30°C for 2 hr. As the number of FTC increased, syneresis was increased; however, ISP significantly (P < 0.05) reduced the syneresis for all FTC except at FTC 1. Hardness was increased up to FTC 4, then decreased for both SG. ISP significantly lowered the hardness of both SG. The ice melting enthalpy (ΔHice sample) of SG increased as FTC increased but SG with ISP had significantly lower enthalpy. The retrogradation ratio (RR) from DSC was significantly increased as the number of FTC increased. ISP reduced RR but most of RR was no...

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