Inheritance of Functional Foreign Genes in Plants

Morphologically normal plants were regenerated from Nicotiana plumbaginifolia cells transformed with an Agrobacterium tumefaciens strain containing a tumor-inducing plasmid with a chimeric gene for kanamycin resistance. The presence of the chimeric gene in regenerated plants was demonstrated by Southern hybridization analysis, and its expression in plant tissues was confirmed by the ability of leaf segments to form callus on media containing kanamycin at concentrations that were normally inhibitory. Progeny derived from several transformed plants inherited the foreign gene in a Mendelian manner.

[1]  P. Sanders,et al.  Expression of bacterial genes in plant cells. , 1983, Proceedings of the National Academy of Sciences of the United States of America.

[2]  M. Chilton,et al.  Short direct repeats flank the T-DNA on a nopaline Ti plasmid. , 1982, Proceedings of the National Academy of Sciences of the United States of America.

[3]  D. Garfinkel,et al.  Genetic analysis of crown gall: Fine structure map of the T-DNA by site-directed mutagenesis , 1981, Cell.

[4]  M. Chilton,et al.  T-DNA of a crown gall teratoma is covalently joined to host plant DNA , 1980, Nature.

[5]  M. Montagu,et al.  DNA from Ti plasmid present in nucleus and absent from plastids of crown gall plant cells , 1980, Nature.

[6]  M. Chilton,et al.  T-DNA from Agrobacterium Ti plasmid is in the nuclear DNA fraction of crown gall tumor cells. , 1980, Proceedings of the National Academy of Sciences of the United States of America.

[7]  M. Chilton,et al.  Stable incorporation of plasmid DNA into higher plant cells: the molecular basis of crown gall tumorigenesis , 1977, Cell.

[8]  A. C. Braun,et al.  Suppression of the neoplastic state with the acquisition of specialized functions in cells, tissues, and organs of crown gall teratomas of tobacco. , 1976, Proceedings of the National Academy of Sciences of the United States of America.

[9]  J. Schell,et al.  Large plasmid in Agrobacterium tumefaciens essential for crown gall-inducing ability , 1974, Nature.

[10]  A. C. Braun.  The activation of two growth-substance systems accompanying the conversion of normal to tumor cells in crown gall. , 1956, Cancer Research.