Natamycin Antibiotic Produced By Streptomyces sp.: Fermentation, Purification and Biological Activities

This work was carried out for the biosynthesis of antifungal substance that demonstrated inhibitory effects against pathogenic fungi from Streptomyces sp. It is active in vitro against some fungal pathogenic viz: S. cerevisiae ATCC 9763; Candida albicans, IMRU 3669; Asp. flavus, , IMI 111023 ; Aspergillus niger IMI 31276; Aspergillus fumigatus ATCC 16424; Aspergillus flavus IMI 111023; Fusarium oxysporum; Rhizoctonia solani; Alternaria alternata; Botrytis fabae and Penicillium chrysogenium. The active metabolite was extracted using nButanol (1:1, v/v) at pH 7.0. The separation of the active ingredient of the antifungal agent and its purification was performed using both thin layer chromatography (TLC) and column chromatography (CC) techniques. The physicochemical characteristics of the purified antibiotic viz. color, melting point, solubility, elemental analysis (C, H, N, O & S) and spectroscopic characteristics (UV absorbance and IR, Mass & NMR spectra) have been investigated. This analysis indicates a suggested empirical formula of C33H47NO13. The minimum inhibition concentrations "MICs" of the purified antifungal agent were also determined. The purified antifungal agent was suggestive of being belonging to Natamycin "polyene" antibiotic produced by Streptomyces sp. [Houssam M. Atta; Sh. M. Selim and Mona S. Zayed]. [Natamycin Antibiotic Produced By Streptomyces sp.: Fermentation, Purification and Biological Activities]. [Journal of American Science 2012;8(2):469-475]. (ISSN: 1545-1003). http://www.americanscience.org. 65

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