In vitro measurements of absolute blood oxygen saturation using pulsed near-infrared photoacoustic spectroscopy: accuracy and resolution.

Pulsed photoacoustic spectroscopy was used to measure blood oxygen saturation in vitro. An optical parametric oscillator laser system provided nanosecond excitation pulses over the wavelength range 740-1040 nm which were used to generate photoacoustic signals in a cuvette through which a saline suspension of red blood cells was circulated. The signal amplitude and the effective attenuation coefficient were extracted from the photoacoustic signals as a function of wavelength to provide photoacoustic spectra of the blood. From these, the relative concentrations of oxy- and deoxyhaemoglobin, and therefore blood oxygen saturation (SO2), were determined using forward models of the absorbed energy distribution based on diffusion theory. A standard linear model of the dependence of absorbance on the concentration of chromophores was also used to calculate the blood oxygen saturation from the signal amplitude spectra. The diffusion approximation model was shown to produce the highest accuracy in blood SO2. The photoacoustically determined oxygen saturation was found to have an accuracy of +/-4% SO2 for signal amplitude data and +/-2.5% SO2 for effective attenuation spectra. The smallest change in oxygen saturation that can be measured using this technique was +/-1% SO2.

[1]  Massoud Motamedi,et al.  Optoacoustic technique for noninvasive monitoring of blood oxygenation: a feasibility study. , 2002, Applied optics.

[2]  Alexander A. Oraevsky,et al.  Optical properties of blood at various levels of oxygenation studied by time-resolved detection of laser-induced pressure profiles , 2002, SPIE BiOS.

[3]  L Wang,et al.  MCML--Monte Carlo modeling of light transport in multi-layered tissues. , 1995, Computer methods and programs in biomedicine.

[4]  A. P. Shepherd,et al.  Comparison of Mie theory and the light scattering of red blood cells. , 1988, Applied optics.

[5]  D. Delpy,et al.  Characterization of the near infrared absorption spectra of cytochrome aa3 and haemoglobin for the non-invasive monitoring of cerebral oxygenation. , 1988, Biochimica et biophysica acta.

[6]  Paul C. Beard,et al.  Pulsed near-infrared photoacoustic spectroscopy of blood , 2004, SPIE BiOS.

[7]  Geng Ku,et al.  Noninvasive functional photoacoustic tomography of blood-oxygen saturation in the brain , 2004, SPIE BiOS.

[8]  R B BARLOW,et al.  Absorption measurements for oxygenated and reduced hemoglobin in the range 0.6-1.88 microns. , 1962, Clinical chemistry.

[9]  B. Horecker.  THE ABSORPTION SPECTRA OF HEMOGLOBIN AND ITS DERIVATIVES IN THE VISIBLE AND NEAR INFRA-RED REGIONS , 1943 .

[10]  Martin Frenz,et al.  Spectral optoacoustic imaging using a scanning transducer , 2001, European Conference on Biomedical Optics.

[11]  Paul C. Beard.  Photoacoustic imaging of blood vessel equivalent phantoms , 2002, SPIE BiOS.

[12]  John C. Murphy,et al.  Local determination of hemoglobin concentration and degree of oxygenation in tissue by pulsed photoacoustic spectroscopy , 2000, BiOS.

[13]  Setsuo Takatani,et al.  Theoretical Analysis of Diffuse Reflectance from a Two-Layer Tissue Model , 1979, IEEE Transactions on Biomedical Engineering.

[14]  Lihong V. Wang,et al.  Noninvasive laser-induced photoacoustic tomography for structural and functional in vivo imaging of the brain , 2003, Nature Biotechnology.

[15]  Roy G. M. Kolkman,et al.  In vivo photoacoustic imaging of blood vessels using an extreme-narrow aperture sensor , 2003 .

[16]  A. Roggan,et al.  Optical Properties of Circulating Human Blood in the Wavelength Range 400-2500 nm. , 1999, Journal of biomedical optics.

[17]  Stefan Andersson-Engels,et al.  Influence of cell shape and aggregate formation on the optical properties of flowing whole blood. , 2003, Applied optics.

[18]  W. Weaver,et al.  Diffusion approximation for large absorption in radiative transfer. , 1979, Applied optics.

[19]  P C Beard.  Interrogation of free-space Fabry–Perot sensing interferometers by angle tuning , 2003 .

[20]  E. Thamm,et al.  Single scattering by red blood cells. , 1998, Applied optics.

[21]  S L Jacques,et al.  CONV--convolution for responses to a finite diameter photon beam incident on multi-layered tissues. , 1997, Computer methods and programs in biomedicine.

[22]  K. Diem.  Documenta Geigy Scientific Tables , 1970 .

[23]  Daniel R. Reinecke,et al.  Thermoacoustic in vivo determination of blood oxygenation , 2004, SPIE BiOS.

[24]  M. Schweiger,et al.  A finite element approach for modeling photon transport in tissue. , 1993, Medical physics.

[25]  G. M. Hale,et al.  Optical Constants of Water in the 200-nm to 200-microm Wavelength Region. , 1973, Applied optics.

[26]  Rebecca Lynn Johnson,et al.  Erythrocyte volume distribution in normal and abnormal subjects. , 1975, Blood.

[27]  Gerhard J. Mueller,et al.  Optical properties of circulating human blood , 1998, European Conference on Biomedical Optics.

[28]  Ashleyj . Welch,et al.  Optical-Thermal Response of Laser-Irradiated Tissue , 1995 .

[29]  F. Perennes,et al.  Transduction mechanisms of the Fabry-Perot polymer film sensing concept for wideband ultrasound detection , 1999, IEEE Transactions on Ultrasonics, Ferroelectrics and Frequency Control.

[30]  Roy G. M. Kolkman,et al.  In vivo photoacoustic imaging of blood vessels with a pulsed laser diode , 2006, Lasers in Medical Science.