Effects of metabolic inhibitors on mouse preimplantation embryo development and the energy metabolism of isolated inner cell masses

The effects of two metabolic inhibitors, methyl palmoxirate (MP) and amino‐oxyacetate (AOA), on mouse preimplantation embryo development and cell number, and inner cell mass (ICM) cell metabolism have been examined. Two‐cell embryos were cultured in media supplemented with either MP, which inhibits fatty acid oxidation, or AOA, which inhibits the transamination of glutamate into α‐ketoglutarate. Embryos were scored for development daily. On day 5, expanded blastocysts were differentially labeled with fluorochromes to visualize TE and ICM cell nuclei, or the ICMs isolated by immunosurgery and their energy metabolism determined using microfluorometric methods.

[1]  H. Leese.  Metabolic control during preimplantation mammalian development. , 1995, Human reproduction update.

[2]  H. Leese,et al.  Uptake of a mixture of amino acids by mouse blastocysts. , 1994, Journal of reproduction and fertility.

[3]  H. Leese,et al.  Energy metabolism of the trophectoderm and inner cell mass of the mouse blastocyst. , 1993, The Journal of experimental zoology.

[4]  D. Gardner,et al.  Amino acids and ammonium regulate mouse embryo development in culture. , 1993, Biology of reproduction.

[5]  S. McKiernan,et al.  Regulation of Hamster Embryo Development In Vitro by Amino Acids , 1993 .

[6]  N. Winston,et al.  Effects of glucose, glutamine, ethylenediaminetetraacetic acid and oxygen tension on the concentration of reactive oxygen species and on development of the mouse preimplantation embryo in vitro. , 1992, Journal of reproduction and fertility.

[7]  R. J. Tasca,et al.  Glutamine uptake and utilization by preimplantation mouse embryos in CZB medium. , 1990, Journal of reproduction and fertility.

[8]  A. Archibong,et al.  Glucose, glutamine and inorganic phosphate in early development of the pig embryo in vitro. , 1990, Journal of reproduction and fertility.

[9]  J. Williams,et al.  Preliminary characterization of pyruvate uptake by one-cell ovine embryos. , 1990, Theriogenology.

[10]  H. Leese,et al.  Concentrations of nutrients in mouse oviduct fluid and their effects on embryo development and metabolism in vitro. , 1990, Journal of reproduction and fertility.

[11]  J. Biggers,et al.  Development of a noninvasive ultramicrofluorometric method for measuring net uptake of glutamine by single preimplantation mouse embryos. , 1989, Gamete research.

[12]  A. Handyside,et al.  The human blastocyst: cell number, death and allocation during late preimplantation development in vitro. , 1989, Development.

[13]  J. Lewis,et al.  An improved culture medium supports development of random-bred 1-cell mouse embryos in vitro. , 1989, Journal of reproduction and fertility.

[14]  D. Rieger,et al.  Measurement of the metabolism of energy substrates in individual bovine blastocysts. , 1988, Journal of reproduction and fertility.

[15]  M. Kane.  In Vitro Growth of Preimplantation Rabbit Embryos , 1987 .

[16]  H. Leese,et al.  Non-invasive measurement of nutrient uptake by single cultured pre-implantation mouse embryos. , 1986, Human reproduction.

[17]  Anthony N. Martonosi,et al.  The Enzymes of Biological Membranes , 1985, Springer US.

[18]  L. Wiley.  Cavitation in the mouse preimplantation embryo: Na/K-ATPase and the origin of nascent blastocoele fluid. , 1984, Developmental biology.

[19]  H. Leese,et al.  Pyruvate and glucose uptake by mouse ova and preimplantation embryos. , 1984, Journal of reproduction and fertility.

[20]  A. Handyside,et al.  A rapid procedure for visualising the inner cell mass and trophectoderm nuclei of mouse blastocysts in situ using polynucleotide-specific fluorochromes. , 1984, The Journal of experimental zoology.

[21]  L. Weaner,et al.  Identification of 2-tetradecylglycidyl coenzyme A as the active form of methyl 2-tetradecylglycidate (methyl palmoxirate) and its characterization as an irreversible, active site-directed inhibitor of carnitine palmitoyltransferase A in isolated rat liver mitochondria. , 1984, The Journal of biological chemistry.

[22]  D. Benos,et al.  Blastocyst Fluid Formation , 1981 .

[23]  H. Leese.  The stimulation of pyruvate appearance in the rabbit oviduct lumen by sodium propionate. , 1980, Journal of reproduction and fertility.

[24]  A. Spindle,et al.  Role of the blastocoele microenvironment in early mouse embryo differentiation , 1980, Nature.

[25]  E. Mroz,et al.  Fluorescence analysis of picoliter samples. , 1980, Analytical biochemistry.

[26]  O. H. Lowry,et al.  Measurement of metabolites in single preimplantation embryos; a new means to study metabolic control in early embryos. , 1978, Journal of embryology and experimental morphology.

[27]  J. Biggers,et al.  Studies on the composition and formation of mouse blastocoele fluid using electron probe microanalysis. , 1977, Developmental biology.

[28]  D. Solter,et al.  Immunosurgery of mouse blastocyst. , 1975, Proceedings of the National Academy of Sciences of the United States of America.

[29]  G. Nicolson,et al.  Ultrastructural localization of lectin-binding sites on the zonae pellucidae and plasma membranes of mammalian eggs , 1975, The Journal of cell biology.

[30]  R. Foote,et al.  Utilization of glutamine for energy and protein synthesis by cultured rabbit follicular oocytes. , 1975, Experimental cell research.

[31]  A. Spindle,et al.  Hatching, attachment, and outgrowth of mouse blastocysts in vitro: fixed nitrogen requirements. , 1973, The Journal of experimental zoology.

[32]  P. Quinn,et al.  Uptake and metabolism of pyruvate and lactate during preimplantation development of the mouse embryo in vitro. , 1973, Journal of reproduction and fertility.

[33]  R. Gwatkin,et al.  Requirements for the maturation of hamster oocytes in vitro. , 1973, Experimental cell research.

[34]  R. Gwatkin.  Amino acid requirements for attachment and outgrowth of the mouse blastocyst in vitro , 1966 .