In vivo confocal scanning laser microscopy in dermatology

The need to improve the diagnostic accuracy and sensitivity for skin tumours has led to the development of new non-invasive, in vivo techniques including ultrasound, dermoscopy, digital photography, confocal scanning laser microscopy, magnetic resonance imaging and optical coherence tomography. Of all these in vivo techniques, only confocal scanning laser microscopy allows for the examination of the epidermis and papillary dermis at a resolution approaching histological detail. This review article looks at some of the most important applications of this new technology, highlighting its qualities and limitations.

[1]  M Rajadhyaksha,et al.  Topographic variations in normal skin, as viewed by in vivo reflectance confocal microscopy. , 2001, The Journal of investigative dermatology.

[2]  A. Marghoob,et al.  In vivo confocal scanning laser microscopy of a series of congenital melanocytic nevi suggestive of having developed malignant melanoma. , 2005, Archives of dermatology.

[3]  S. González,et al.  Confocal histopathology of irritant contact dermatitis in vivo and the impact of skin color (black vs white). , 2003, Journal of the American Academy of Dermatology.

[4]  P Altmeyer,et al.  Effects of repeated sunbed exposures on the human skin. In vivo measurements with confocal microscopy , 2004, Photodermatology, photoimmunology & photomedicine.

[5]  M. Rajadhyaksha,et al.  Confocal imaging of sebaceous gland hyperplasia in vivo to assess efficacy and mechanism of pulsed dye laser treatment , 1999, Lasers in surgery and medicine.

[6]  P Altmeyer,et al.  Investigation of basal cell carcinoma [correction of carcionoma] by confocal laser scanning microscopy in vivo. , 2002, Skin research and technology : official journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging.

[7]  Cesare Massone,et al.  Confocal examination of untreated fresh specimens from basal cell carcinoma: implications for microscopically guided surgery. , 2005, Archives of dermatology.

[8]  R. Lotan,et al.  Contrast agents for confocal microscopy: how simple chemicals affect confocal images of normal and cancer cells in suspension. , 2002, Journal of biomedical optics.

[9]  M Rajadhyaksha,et al.  Allergic contact dermatitis: correlation of in vivo confocal imaging to routine histology. , 1999, Journal of the American Academy of Dermatology.

[10]  P. Corcuff,et al.  Morphometry of human epidermis in vivo by real-time confocal microscopy , 2004, Archives of Dermatological Research.

[11]  C. Grana,et al.  In vivo confocal scanning laser microscopy of pigmented Spitz nevi: comparison of in vivo confocal images with dermoscopy and routine histopathology. , 2004, Journal of the American Academy of Dermatology.

[12]  Motoji Takahashi,et al.  Non-invasive visualization of melanin and melanocytes by reflectance-mode confocal microscopy. , 2005, The Journal of investigative dermatology.

[13]  A. Marghoob,et al.  Detection of intraepidermal malignant melanoma in vivo by confocal scanning laser microscopy , 2002, Melanoma research.

[14]  Josef Smolle,et al.  Sensitivity and specificity of confocal laser‐scanning microscopy for in vivo diagnosis of malignant skin tumors , 2006, Cancer.

[15]  Allan C Halpern,et al.  Morphologic Features of Melanocytes, Pigmented Keratinocytes, and Melanophages by In Vivo Confocal Scanning Laser Microscopy , 2001, Modern Pathology.

[16]  M Rajadhyaksha,et al.  Multimodal in vivo optical imaging, including confocal microscopy, facilitates presurgical margin mapping for clinically complex lentigo maligna melanoma , 2005, The British journal of dermatology.

[17]  A. Lacy,et al.  Near real time confocal microscopy of amelanotic tissue: detection of dysplasia in ex-vivo cervical tissue , 2002, Proceedings of the Second Joint 24th Annual Conference and the Annual Fall Meeting of the Biomedical Engineering Society] [Engineering in Medicine and Biology.

[18]  E. Piette,et al.  A Textbook of Histology , 1936, The Indian Medical Gazette.

[19]  S. González,et al.  5% Imiquimod Cream and Reflectance‐Mode Confocal Microscopy as Adjunct Modalities to Mohs Micrographic Surgery for Treatment of Basal Cell Carcinoma , 2004, Dermatologic surgery : official publication for American Society for Dermatologic Surgery [et al.].

[20]  M Rajadhyaksha,et al.  Non-invasive (real-time) imaging of histologic margin of a proliferative skin lesion in vivo. , 1998, The Journal of investigative dermatology.

[21]  A. Marghoob,et al.  Reflectance confocal microscopy of pigmented basal cell carcinoma. , 2006, Journal of the American Academy of Dermatology.

[22]  S. González,et al.  In Vivo Real‐Time Confocal Reflectance Microscopy: A Noninvasive Guide for Mohs Micrographic Surgery Facilitated by Aluminum Chloride, an Excellent Contrast Enhancer , 2003, Dermatologic surgery : official publication for American Society for Dermatologic Surgery [et al.].

[23]  T. Gambichler,et al.  Histometric data obtained by in vivo confocal laser scanning microscopy in patients with systemic sclerosis , 2002, BMC dermatology.

[24]  A. Boyde,et al.  In vivo imaging of human teeth and skin using real-time confocal microscopy , 1991 .

[25]  A. Halpern,et al.  Detection of clinically amelanotic malignant melanoma and assessment of its margins by in vivo confocal scanning laser microscopy. , 2001, Archives of dermatology.

[26]  M. Rajadhyaksha,et al.  Use of Ex Vivo Confocal Scanning Laser Microscopy during Mohs Surgery for Nonmelanoma Skin Cancers , 2004, Dermatologic surgery : official publication for American Society for Dermatologic Surgery [et al.].

[27]  R. Anderson,et al.  Confirmation of onychomycosis by in vivo confocal microscopy. , 2000, Journal of the American Academy of Dermatology.

[28]  Alexander A. Maximow,et al.  A Textbook of Histology , 1935, The Indian Medical Gazette.

[29]  C. Longo,et al.  Microscopic in vivo description of cellular architecture of dermoscopic pigment network in nevi and melanomas. , 2005, Archives of dermatology.

[30]  Thilo Gambichler,et al.  Age related changes of human skin investigated with histometric measurements by confocal laser scanning microscopy in vivo , 2002, Skin research and technology : official journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging.

[31]  S. González,et al.  Non-invasive evaluation of the kinetics of allergic and irritant contact dermatitis. , 2005, The Journal of investigative dermatology.

[32]  S. Seidenari,et al.  In vivo confocal reflectance microscopy for the characterization of melanocytic nests and correlation with dermoscopy and histology , 2005, The British journal of dermatology.

[33]  C C Harland,et al.  Skin imaging: is it clinically useful? , 2004, Clinical and experimental dermatology.

[34]  T Wilson,et al.  Depth of field in the scanning microscope. , 1978, Optics letters.

[35]  R. Anderson,et al.  Time-sequence histologic imaging of laser-treated cherry angiomas with in vivo confocal microscopy. , 2000, Journal of the American Academy of Dermatology.

[36]  M. Rajadhyaksha,et al.  Confocal reflectance imaging of folliculitis in vivo: correlation with routine histology , 1999, Journal of cutaneous pathology.

[37]  Ashfaq A Marghoob,et al.  Melanoma or pigmented basal cell carcinoma: a clinical‐pathologic correlation with dermoscopy, in vivo confocal scanning laser microscopy, and routine histology , 2002, Skin research and technology : official journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging.

[38]  P. Corcuff,et al.  In vivo vision of the human skin with the tandem scanning microscope. , 1993, Dermatology.

[39]  Salvador González,et al.  In vivo examination of lentigo maligna and malignant melanoma in situ, lentigo maligna type by near-infrared reflectance confocal microscopy: comparison of in vivo confocal images with histologic sections. , 2002, Journal of the American Academy of Dermatology.

[40]  S. González,et al.  Real-time, in vivo confocal reflectance microscopy of basal cell carcinoma. , 2002, Journal of the American Academy of Dermatology.

[41]  M. Rajadhyaksha,et al.  Characterization of psoriasis in vivo by reflectance confocal microscopy. , 1999, Journal of medicine.

[42]  P Corcuff,et al.  Skin optics revisited by in vivo confocal microscopy: melanin and sun exposure. , 2001, Journal of cosmetic science.

[43]  S. González,et al.  Sensitivity and specificity of reflectance-mode confocal microscopy for in vivo diagnosis of basal cell carcinoma: a multicenter study. , 2004, Journal of the American Academy of Dermatology.

[44]  P Corcuff,et al.  In vivo spatio-temporal visualization of the human skin by real-time confocal microscopy. , 1994, Scanning.

[45]  S. Gonźalezb,et al.  In vivo examination of lentigo maligna and malignant melanoma in situ, lentigo maligna type by near-infrared reflectance confocal microscopy: Comparison of in vivo confocal images with histologic sections ☆ ☆☆ , 2002 .

[46]  R. Anderson,et al.  A better potassium hydroxide preparation? In vivo diagnosis of tinea with confocal microscopy. , 2001, Archives of dermatology.

[47]  R. Anderson,et al.  The optics of human skin. , 1981, The Journal of investigative dermatology.

[48]  S. González,et al.  Confocal microscopy oriented cryosurgery , 2002, International journal of dermatology.

[49]  R. Webb,et al.  In vivo confocal scanning laser microscopy of human skin II: advances in instrumentation and comparison with histology. , 1999, The Journal of investigative dermatology.

[50]  S. González,et al.  Real-time, in vivo quantification of melanocytes by near-infrared reflectance confocal microscopy in the Guinea pig animal model. , 2002, The Journal of investigative dermatology.

[51]  S. González,et al.  Detection of Residual Basal Cell Carcinoma by In Vivo Confocal Microscopy , 2005, Dermatologic surgery : official publication for American Society for Dermatologic Surgery [et al.].

[52]  R. Anderson,et al.  Confocal laser microscopic imaging of actinic keratoses in vivo: a preliminary report. , 2000, Journal of the American Academy of Dermatology.

[53]  S. González,et al.  Use of in vivo confocal microscopy in malignant melanoma: an aid in diagnosis and assessment of surgical and nonsurgical therapeutic approaches. , 2004, Archives of dermatology.

[54]  R. Webb,et al.  In vivo confocal scanning laser microscopy of human skin: melanin provides strong contrast. , 1995, The Journal of investigative dermatology.

[55]  M. Mihm,et al.  Reflectance confocal microscopy may differentiate acute allergic and irritant contact dermatitis in vivo. , 2002, Journal of the American Academy of Dermatology.

[56]  M. Rajadhyaksha,et al.  Elucidating the pulsed-dye laser treatment of sebaceous hyperplasia in vivo with real-time confocal scanning laser microscopy. , 2000, Journal of the American Academy of Dermatology.

[57]  M. Rajadhyaksha,et al.  Confocal scanning laser microscopy of benign and malignant melanocytic skin lesions in vivo. , 2001, Journal of the American Academy of Dermatology.