Biological and environmental conditionings for a sperm DNA fragmentation.

The objective of the presented study was determination of the effect of selected agents on sperm DNA fragmentation--superoxide dismutase in seminal plasma, the patients' age, and burdening with the tobacco smoking habit. An attempt was also undertaken to evaluate the effect of DNA fragmentation on the effectiveness of infertility treatment. The study covered 186 men who received treatment due to infertility. The database and statistical analyses were performed using computer software STATISTICA 7.1. A relationship was observed between sperm DNA fragmentation and superoxide dismutase activity, the higher the SOD activity, the lower the percentage of sperm fragmentation (rs=-0.324; P=0.000; r = -0.2110). A statistical relationship was found between sperm DNA fragmentation and the percentage of pregnancies obtained during the first year of treatment--patients with the lower DFI more frequently became fathers during the first year of trying, compared to the remainder (t=2.51; P=0.013). A statistically significant relationship was confirmed (rs=-0.370; P=0.000) consisting in an increase in the DFI with respondents' age. No significant differences were noted between the DFI and the tobacco smoking habit (Chi2=0.29; P=0.926). The percentage of sperm DNA fragmentation was inversely proportional to superoxide dismutase activity in seminal plasma. DNA fragmentation becomes intensified with patients' age. Cigarette smoking has no effect on sperm DNA fragmentation. DNA fragmentation exerts an effect on the effectiveness of infertility treatment.

[1]  D. Wells,et al.  Increased numbers of DNA-damaged spermatozoa in samples presenting an elevated rate of numerical chromosome abnormalities. , 2013, Human reproduction.

[2]  P. Cieślik,et al.  Erythrocyte antioxidant parameters in workers occupationally exposed to low levels of ionizing radiation. , 2008, Annals of agricultural and environmental medicine : AAEM.

[3]  A. Giwercman,et al.  Sperm chromatin structure assay (SCSA): a tool in diagnosis and treatment of infertility. , 2011, Asian journal of andrology.

[4]  H. Rodríguez-Martínez,et al.  Sperm chromatin structure and sperm morphology: their association with fertility in AI-dairy Ayrshire sires. , 2013, Theriogenology.

[5]  R. Casaccia.  Sperm chromatin structure and male fertility:biological and clinical aspects , 2006 .

[6]  Sunil Kumar,et al.  Association between sperm quality, oxidative stress, and seminal antioxidant activity. , 2011, Clinical biochemistry.

[7]  R. Dada,et al.  Antioxidant levels in blood and seminal plasma and their impact on sperm parameters in infertile men. , 2010, Indian journal of biochemistry & biophysics.

[8]  C. Flamigni,et al.  Sperm DNA fragmentation: paternal effect on early post-implantation embryo development in ART. , 2006, Human reproduction.

[9]  A. Giwercman,et al.  Sperm DNA damage in men from infertile couples. , 2008, Asian journal of andrology.

[10]  Zhao-xia Wang,et al.  The alteration of protein profile induced by cigarette smoking via oxidative stress in mice epididymis. , 2013, The international journal of biochemistry & cell biology.

[11]  S. Arsenijević,et al.  Activity of superoxide dismutase and catalase and content of malondialdehyde in seminal plasma of infertile patients. , 2010, Medicinski pregled.

[12]  F. Chapuis,et al.  Antioxidants to reduce sperm DNA fragmentation: an unexpected adverse effect. , 2007, Reproductive biomedicine online.

[13]  A. Kadıoğlu,et al.  Mystery of idiopathic male infertility: is oxidative stress an actual risk? , 2013, Fertility and sterility.

[14]  J. Saczko,et al.  Evaluation of superoxide dismutase activity and its impact on semen quality parameters of infertile men. , 2007, Folia histochemica et cytobiologica.

[15]  A. Kerkeni,et al.  Effects of reduced seminal enzymatic antioxidants on sperm DNA fragmentation and semen quality of Tunisian infertile men , 2017, Journal of Assisted Reproduction and Genetics.

[16]  M. Dobrzyńska,et al.  Is concentration and motility of male gametes related to DNA damage measured by comet assay? , 2010, Annals of agricultural and environmental medicine : AAEM.

[17]  J. R. González,et al.  Effect of the addition of two superoxide dismutase analogues (Tempo and Tempol) to alpaca semen extender for cryopreservation , 2013 .

[18]  M. Bakhiet,et al.  DNA integrity is a critical molecular indicator for the assessment of male infertility. , 2013, Molecular medicine reports.

[19]  B. Tartibian,et al.  Correlation Between Seminal Oxidative Stress Biomarkers and Antioxidants with Sperm DNA Damage in Elite Athletes and Recreationally Active Men , 2012, Clinical journal of sport medicine : official journal of the Canadian Academy of Sport Medicine.

[20]  M. Godycki-Cwirko,et al.  PREVALENCE OF CIGARETTE SMOKING AMONG ADULT POPULATION IN EASTERN POLAND , 2010 .