Procedure for simultaneous phenotyping of genetic variants in cow's milk by isoelectric focusing.

A method is described which allows the simultaneous separation of all polymorphic protein fractions in cow's milk in one single run. The separation could be achieved by isoelectric focusing in ultrathin-layer polyacrylamide gels. The method is especially suitable for screening purposes because it combines low costs, high resolution and short separation time.

[1]  C. A. Ernstrom,et al.  Nomenclature of Proteins of Cow's Milk: Fifth Revision , 1984 .

[2]  A. Andrews,et al.  Proteolysis of caseins and the proteose-peptone fraction of bovine milk , 1983, Journal of Dairy Research.

[3]  P. Trieu-Cuot,et al.  Electrofocusing and two-dimensional electrophoresis of bovine caseins , 1981, Journal of Dairy Research.

[4]  J. R. Brunner,et al.  Cow Milk Proteins: Twenty-Five Years of Progress ~ , 1981 .

[5]  B. Radola.  Ultrathin‐layer isoelectric focusing in 50–100 μm polyacrylamide gels on silanized glass plates or polyester films , 1980 .

[6]  W. J. Gelsema,et al.  Isoelectric points of proteins, determined by isoelectric focusing in the presence of urea and ethanol. , 1979, Journal of chromatography.

[7]  R. Josephson.  Isoelectric focusing of bovine milk caseins. , 1972, Journal of dairy science.

[8]  J. P. Zikakis,et al.  Discontinuous polyacrylamide electrophoresis for -lactoglobulin typing of cow's milk. , 1972, Journal of dairy science.

[9]  M. P. Thompson.  Phenotyping milk proteins. A review. , 1970, Journal of dairy science.

[10]  R. Aschaffenburg,et al.  Simultaneous phenotyping procedure for milk proteins--improved resolution of the beta-lactoglobulins. , 1968, Journal of dairy science.

[11]  C. W. Arave.  Procedure for Simultaneous Phenotyping of β-Casein and β-Lactoglobulin Variants in Cow's Milk , 1967 .

[12]  M. Thymann,et al.  Studies on Milk Protein Polymorphism in Danish Cattle and the Interaction of the Controlling Genes , 1966, Acta Veterinaria Scandinavica.

[13]  F. Kopfler,et al.  Detection of new types of β-casein by polyacrylamide gel electrophoresis at acid pH: A proposed nomenclature , 1966 .

[14]  R. Aschaffenburg,et al.  Simultaneous Phenotyping Procedure for the Principal Proteins of Cow's Milk , 1965 .

[15]  R. L. Baldwin,et al.  Analysis of casein fractions by zone electrophoresis in concentrated urea. , 1961, Biochimica et biophysica acta.

[16]  R. Aschaffenburg,et al.  Occurrence of Different Beta-Lactoglobulins in Cow's Milk , 1955, Nature.