Characterization of polylactic acid-polyglycolic acid composites for cartilage tissue engineering.

The objective of this study was to determine the effects of scaffold composition on the physical properties, adhesion, and growth of bovine articular chondrocytes on polylactic acid (PLA)/polyglycolic acid (PGA) composites. Nonwoven meshes of PGA were coated with PLA, using a solvent evaporation technique that resulted in composites with fractional PLA contents ranging from 0 to 68%. The compressive modulus of scaffolds increased linearly with the addition of PLA, ranging from less than 1 kPa for PGA to approximately 20 kPa for scaffolds with 68% PLA content. The characteristic degradation time of these scaffolds also increased from approximately 5 days for 0% PLA to 45 days for 68% PLA. Addition of PLA decreased cell seeding efficiency from 48% for 0% PLA scaffolds to 27% for 68% PLA scaffolds. Cells seeded onto 27% PLA scaffolds increased 3-fold in number over 4 weeks in culture, whereas cells seeded onto 68% PLA increased only 2-fold in number. Scanning electron microscopy indicated that cells attached to PGA appeared flat with many small processes, whereas those attached to PLA were more rounded. These studies provide important information for the design of scaffolds for cartilage tissue engineering.

[1]  A. Grodzinsky,et al.  Fluorometric assay of DNA in cartilage explants using Hoechst 33258. , 1988, Analytical biochemistry.

[2]  R Langer,et al.  Design of nasoseptal cartilage replacements synthesized from biodegradable polymers and chondrocytes. , 1994, Biomaterials.

[3]  Boon Chin Heng,et al.  Directing Stem Cell Differentiation into the Chondrogenic Lineage In Vitro , 2004, Stem cells.

[4]  P. Ma,et al.  Biodegradable polymer scaffolds with well-defined interconnected spherical pore network. , 2001, Tissue engineering.

[5]  Daniel I. C. Wang,et al.  Engineering cell shape and function. , 1994, Science.

[6]  A. Grodzinsky,et al.  Cartilage electromechanics--I. Electrokinetic transduction and the effects of electrolyte pH and ionic strength. , 1987, Journal of biomechanics.

[7]  Karine Anselme,et al.  Repair of osteochondral defects with autologous chondrocytes seeded onto bioceramic scaffold in sheep. , 2004, Tissue engineering.

[8]  D J Mooney,et al.  Injection molding of chondrocyte/alginate constructs in the shape of facial implants. , 2001, Journal of biomedical materials research.

[9]  Clemente Ibarra,et al.  Characteristics of cartilage engineered from human pediatric auricular cartilage. , 1999, Plastic and reconstructive surgery.

[10]  J. Vacanti,et al.  Experimental tracheal replacement using tissue-engineered cartilage. , 1994, Journal of pediatric surgery.

[11]  B. Obradovic,et al.  Bioreactor cultivation conditions modulate the composition and mechanical properties of tissue‐engineered cartilage , 1999, Journal of orthopaedic research : official publication of the Orthopaedic Research Society.

[12]  Charles A Vacanti,et al.  Tissue-Engineered Composites of Anulus Fibrosus and Nucleus Pulposus for Intervertebral Disc Replacement , 2004, Spine.

[13]  M. B. Claase,et al.  The different behaviors of skeletal muscle cells and chondrocytes on PEGT/PBT block copolymers are related to the surface properties of the substrate. , 2001, Journal of biomedical materials research.

[14]  Morgan E. Hott,et al.  Fabrication of Tissue Engineered Tympanic Membrane Patches Using Computer‐Aided Design and Injection Molding , 2004, The Laryngoscope.

[15]  Kyriacos A Athanasiou,et al.  Comparison of scaffolds and culture conditions for tissue engineering of the knee meniscus. , 2005, Tissue engineering.

[16]  L. Bonassar,et al.  Replacement of an avulsed phalanx with tissue-engineered bone. , 2001, The New England journal of medicine.

[17]  Charles A Vacanti,et al.  Tissue Engineering of Autologous Cartilage for Craniofacial Reconstruction by Injection Molding , 2003, Plastic and reconstructive surgery.

[18]  L. Bonassar,et al.  Age dependence of cellular properties of human septal cartilage: implications for tissue engineering. , 2001, Archives of otolaryngology--head & neck surgery.

[19]  D J Mooney,et al.  Engineering smooth muscle tissue with a predefined structure. , 1998, Journal of biomedical materials research.

[20]  R Langer,et al.  Neocartilage formation in vitro and in vivo using cells cultured on synthetic biodegradable polymers. , 1993, Journal of biomedical materials research.

[21]  Woo Seob Kim,et al.  Cartilage Engineered in Predetermined Shapes Employing Cell Transplantation on Synthetic Biodegradable Polymers , 1994, Plastic and reconstructive surgery.

[22]  A. Grodzinsky,et al.  Molecular electromechanics of cartilaginous tissues and polyelectrolyte gels , 1995 .

[23]  J. Vacanti,et al.  In vitro degradation of porous poly(L-lactic acid) foams. , 2000, Biomaterials.

[24]  D Amiel,et al.  The use of polylactic acid matrix and periosteal grafts for the reconstruction of rabbit knee articular defects. , 1991, Journal of biomedical materials research.

[25]  R. Langer,et al.  The stimulation of DNA synthesis and cell division in chondrocytes and 3T3 cells by a growth factor isolated from cartilage. , 1977, Experimental cell research.

[26]  Charles A. Vacanti,et al.  Transplantation of Chondrocytes Utilizing a Polymer‐Cell Construct to Produce Tissue‐Engineered Cartilage in the Shape of a Human Ear , 1997, Plastic and reconstructive surgery.

[27]  H Planck,et al.  Cartilage reconstruction in head and neck surgery: comparison of resorbable polymer scaffolds for tissue engineering of human septal cartilage. , 1998, Journal of biomedical materials research.

[28]  L. Bonassar,et al.  Comparison of Chondrogensis in Static and Perfused Bioreactor Culture , 2000, Biotechnology progress.

[29]  D. Carnes,et al.  Pretreatment with platelet derived growth factor-BB modulates the ability of costochondral resting zone chondrocytes incorporated into PLA/PGA scaffolds to form new cartilage in vivo. , 2000, Biomaterials.

[30]  P. Tresco,et al.  Relative importance of surface wettability and charged functional groups on NIH 3T3 fibroblast attachment, spreading, and cytoskeletal organization. , 1998, Journal of biomedical materials research.