The influence of structural design of PLGA/collagen hybrid scaffolds in cartilage tissue engineering.

3D biodegradable porous scaffold plays a very important role in articular cartilage tissue engineering. We developed hybrid structures of 3D scaffolds that combined the advantages of natural type I collagen and synthetic PLGA knitted mesh. The mechanically strong PLGA mesh served as a skeleton while the collagen microsponges facilitated cell seeding and tissue formation. The scaffolds were divided into 3 groups: (1) THIN: collagen microsponge formed in interstices of PLGA mesh; (2) SEMI: collagen microsponge formed on one side of PLGA mesh; (3) SANDWICH: collagen sponge formed on both sides of PLGA mesh. Bovine chondrocytes were cultured in these scaffolds and transplanted subcutaneously into nude mice for 2, 4, and 8 weeks. All three groups of transplants showed homogeneous cell distribution, natural chondrocyte morphology, and abundant cartilaginous ECM deposition. Production of GAGs per DNA and the expression of type II collagen and aggrecan mRNA were much higher in the SEMI and SANDWICH groups than in the THIN group. When compared to native articular cartilage, the mechanical strength of the engineered cartilage reached 54.8%, 49.3% in Young's modulus and 68.8%, 62.7% in stiffness, respectively, in SEMI and SANDWICH. These scaffolds could be used for the tissue engineering of articular cartilage with adjustable thickness. The design of the hybrid structures provides a strategy for the preparation of 3D porous scaffolds.

[1]  S. Kili.  Mechanical testing of fixation techniques for scaffold-based tissue-engineered grafts. , 2008, Journal of biomedical materials research. Part B, Applied biomaterials.

[2]  W. Richter,et al.  An in vivo study of a growth-factor enhanced, cell free, two-layered collagen-tricalcium phosphate in deep osteochondral defects. , 2006, Biomaterials.

[3]  W. Dunn,et al.  Treatment of Focal Articular Cartilage Defects in the Knee , 2008, Clinical orthopaedics and related research.

[4]  R. Kandel,et al.  The use of specific chondrocyte populations to modulate the properties of tissue‐engineered cartilage , 2003, Journal of orthopaedic research : official publication of the Orthopaedic Research Society.

[5]  A. Grodzinsky,et al.  Effects of a cultured autologous chondrocyte‐seeded type II collagen scaffold on the healing of a chondral defect in a canine model , 2003, Journal of orthopaedic research : official publication of the Orthopaedic Research Society.

[6]  Moon Suk Kim,et al.  An in vivo study of the host tissue response to subcutaneous implantation of PLGA- and/or porcine small intestinal submucosa-based scaffolds. , 2007, Biomaterials.

[7]  Wei Liu,et al.  Collagen Tissue Engineering: Development of Novel Biomaterials and Applications , 2008, Pediatric Research.

[8]  Lars Engebretsen,et al.  Clinical application of scaffolds for cartilage tissue engineering , 2008, Knee Surgery, Sports Traumatology, Arthroscopy.

[9]  R M Nerem,et al.  Tissue engineering: from biology to biological substitutes. , 1995, Tissue engineering.

[10]  Makarand V Risbud,et al.  Tissue engineering: advances in in vitro cartilage generation. , 2002, Trends in biotechnology.

[11]  T. Uemura,et al.  Stimulation of osteogenic activity in mesenchymal stem cells by FK506. , 2008, Journal of biomedical materials research. Part A.

[12]  E. Sachlos,et al.  Making tissue engineering scaffolds work. Review: the application of solid freeform fabrication technology to the production of tissue engineering scaffolds. , 2003, European cells & materials.

[13]  Scott Hollister,et al.  Tissue-engineered cartilage constructs using composite hyaluronic acid/collagen I hydrogels and designed poly(propylene fumarate) scaffolds. , 2007, Tissue engineering.

[14]  Bernd Baumann,et al.  Chondrogenic differentiation of human mesenchymal stem cells in collagen type I hydrogels. , 2007, Journal of biomedical materials research. Part A.

[15]  E B Hunziker,et al.  Articular cartilage repair: basic science and clinical progress. A review of the current status and prospects. , 2002, Osteoarthritis and cartilage.

[16]  Scott C. Brown,et al.  A three-dimensional osteochondral composite scaffold for articular cartilage repair. , 2002, Biomaterials.

[17]  Pauline M Doran,et al.  Tissue engineering of human cartilage and osteochondral composites using recirculation bioreactors. , 2005, Biomaterials.

[18]  L. Hangody,et al.  Autologous Osteochondral Mosaicplasty for the Treatment of Full-Thickness Defects of Weight-Bearing Joints: Ten Years of Experimental and Clinical Experience , 2003, The Journal of bone and joint surgery. American volume.

[19]  Stephen B Doty,et al.  In vivo evaluation of a multiphased scaffold designed for orthopaedic interface tissue engineering and soft tissue-to-bone integration. , 2008, Journal of biomedical materials research. Part A.

[20]  D. Magne,et al.  Cartilage formation in growth plate and arteries: from physiology to pathology , 2005, BioEssays : news and reviews in molecular, cellular and developmental biology.

[21]  W. Uhl,et al.  IN VITRO COMPARISON OF THREE DIFFERENT MESH CONSTRUCTIONS , 2008, ANZ journal of surgery.

[22]  Characteristics of Tissue‐Engineered Cartilage on Macroporous Biodegradable PLGA Scaffold , 2006, The Laryngoscope.

[23]  Ching-Chuan Jiang,et al.  Repair of articular cartilage defects: review and perspectives. , 2009, Journal of the Formosan Medical Association = Taiwan yi zhi.

[24]  Koji Hattori,et al.  Cartilage regeneration using mesenchymal stem cells and a three-dimensional poly-lactic-glycolic acid (PLGA) scaffold. , 2005, Biomaterials.

[25]  J. Mason,et al.  Cartilage tissue engineering: current limitations and solutions. , 1999, Clinical orthopaedics and related research.

[26]  K A Athanasiou,et al.  Basic science of articular cartilage repair. , 2001, Clinics in sports medicine.

[27]  C. L. Murphy,et al.  Topographical variation in glycosaminoglycan content in human articular cartilage. , 2006, The Journal of bone and joint surgery. British volume.

[28]  Yu Suk Choi,et al.  Adipose tissue engineering using mesenchymal stem cells attached to injectable PLGA spheres. , 2005, Biomaterials.

[29]  R E Guldberg,et al.  Variations in matrix composition and GAG fine structure among scaffolds for cartilage tissue engineering. , 2005, Osteoarthritis and cartilage.

[30]  Takehisa Matsuda,et al.  Mesoscopic spatial designs of nano- and microfiber meshes for tissue-engineering matrix and scaffold based on newly devised multilayering and mixing electrospinning techniques. , 2005, Biomaterials.

[31]  Robert Langer,et al.  Polymeric Biomaterials in Tissue Engineering , 2008, Pediatric Research.

[32]  W. Widuchowski,et al.  Untreated Asymptomatic Deep Cartilage Lesions Associated with Anterior Cruciate Ligament Injury , 2009, The American journal of sports medicine.

[33]  David J Mooney,et al.  New materials for tissue engineering: towards greater control over the biological response. , 2008, Trends in biotechnology.

[34]  B. Swoboda,et al.  Measurement of cartilage thickness in the human knee-joint by magnetic resonance imaging using a three-dimensional gradient-echo sequence , 1999, International Orthopaedics.

[35]  V C Mow,et al.  Quantitation of articular surface topography and cartilage thickness in knee joints using stereophotogrammetry. , 1991, Journal of biomechanics.

[36]  F P T Baaijens,et al.  Design of scaffolds for blood vessel tissue engineering using a multi-layering electrospinning technique. , 2005, Acta biomaterialia.

[37]  G. Vunjak‐Novakovic,et al.  Composition of cell‐polymer cartilage implants , 1994, Biotechnology and bioengineering.

[38]  Guoping Chen,et al.  The use of a novel PLGA fiber/collagen composite web as a scaffold for engineering of articular cartilage tissue with adjustable thickness. , 2003, Journal of biomedical materials research. Part A.

[39]  Adam R. Johnson,et al.  〈 Review 〉 Cartilage Repair Current and Emerging Options in Treatment , 2009 .

[40]  A. Bhosale,et al.  Articular cartilage: structure, injuries and review of management. , 2008, British medical bulletin.

[41]  Guoping Chen,et al.  Evaluation of diaphragmatic hernia repair using PLGA mesh–collagen sponge hybrid scaffold: an experimental study in a rat model , 2008, Pediatric Surgery International.

[42]  Julie Glowacki,et al.  Collagen scaffolds for tissue engineering. , 2008, Biopolymers.

[43]  Z. Gugala,et al.  In vitro growth and activity of primary chondrocytes on a resorbable polylactide three-dimensional scaffold. , 2000, Journal of biomedical materials research.

[44]  E. Bueno,et al.  Hydrodynamic parameters modulate biochemical, histological, and mechanical properties of engineered cartilage. , 2009, Tissue engineering. Part A.