Multiple fluorescence in situ hybridization.

A method for multiple fluorescence in situ hybridization is described allowing the simultaneous detection of more than three target sequences with only three fluorescent dyes (FITC, TRITC, AMCA), respectively emitting in the green, red, and blue. This procedure is based on the labeling of (DNA) probes with more than one hapten and visualisation in multiple colors. The possibility to detect multiple targets simultaneously is important for prenatal diagnosis and the detection of numerical and/or structural chromosome aberrations in tumor diagnosis. It may form the basis for an in situ hybridization based chromosome banding technique.

[1]  E. I. Budowsky,et al.  Modification of cytidine residues with a bisulfite-O-methylhydroxylamine mixture. , 1974, Biochimica et biophysica acta.

[2]  D C Ward,et al.  Enzymatic synthesis of biotin-labeled polynucleotides: novel nucleic acid affinity probes. , 1981, Proceedings of the National Academy of Sciences of the United States of America.

[3]  M. van der Ploeg,et al.  2-Acetylaminofluorene-modified probes for the indirect hybridocytochemical detection of specific nucleic acid sequences. , 1984, Experimental cell research.

[4]  T M Jovin,et al.  Fluorescence digital imaging microscopy in cell biology. , 1985, Science.

[5]  G. Poste,et al.  Improved flow cytometric analysis of leukocyte subsets: simultaneous identification of five cell subsets using two-color immunofluorescence. , 1986, Proceedings of the National Academy of Sciences of the United States of America.

[6]  P. Devilee,et al.  Two subsets of human alphoid repetitive DNA show distinct preferential localization in the pericentric regions of chromosomes 13, 18, and 21. , 1986, Cytogenetics and cell genetics.

[7]  H. Willard,et al.  Molecular analysis of a deletion polymorphism in alpha satellite of human chromosome 17: evidence for homologous unequal crossing-over and subsequent fixation. , 1986, Nucleic acids research.

[8]  H. Willard,et al.  Genomic organization of alpha satellite DNA on human chromosome 7: evidence for two distinct alphoid domains on a single chromosome , 1987, Molecular and cellular biology.

[9]  D. Pinkel,et al.  Fluorescence in situ hybridization with human chromosome-specific libraries: detection of trisomy 21 and translocations of chromosome 4. , 1988, Proceedings of the National Academy of Sciences of the United States of America.

[10]  T. Cremer,et al.  Rapid interphase and metaphase assessment of specific chromosomal changes in neuroectodermal tumor cells by in situ hybridization with chemically modified DNA probes. , 1988, Experimental cell research.

[11]  H. Willard,et al.  Detection of chromosome aneuploidy in interphase nuclei from human primary breast tumors using chromosome-specific repetitive DNA probes. , 1988, Cancer research.

[12]  P. Nederlof,et al.  Three-color fluorescence in situ hybridization for the simultaneous detection of multiple nucleic acid sequences. , 1989, Cytometry.

[13]  H. Tanke,et al.  Detection of chromosome aberrations in interphase tumor nuclei by nonradioactive in situ hybridization. , 1989, Cancer genetics and cytogenetics.

[14]  A. Waggoner,et al.  Cyanine dye labeling reagents for sulfhydryl groups. , 1989, Cytometry.

[15]  P. Devilee,et al.  Direct nonradioactive in situ hybridization of somatic cell hybrid DNA to human lymphocyte chromosomes. , 1990, Cytometry.