Efficient Sampling of Protein Sequence Space for Multiple Mutants

[1]  J. Sambrook,et al.  Molecular Cloning: A Laboratory Manual , 2001 .

[2]  D. Youvan,et al.  Searching Sequence Space to Engineer Proteins: Exponential Ensemble Mutagenesis , 1993, Bio/Technology.

[3]  K. Struhl,et al.  Regional codon randomization: defining a TATA-binding protein surface required for RNA polymerase III transcription. , 1993, Science.

[4]  D. Shortle,et al.  Patterns of nonadditivity between pairs of stability mutations in staphylococcal nuclease. , 1993, Biochemistry.

[5]  G. Winter,et al.  Combinatorial infection and in vivo recombination: a strategy for making large phage antibody repertoires. , 1993, Nucleic acids research.

[6]  H. Lowman,et al.  Rapid evolution of peptide and protein binding properties in vitro , 1992, Current Biology.

[7]  David J Weber,et al.  Quantitative interpretations of double mutations of enzymes. , 1992, Archives of biochemistry and biophysics.

[8]  C. Barbas,et al.  In vitro selection and affinity maturation of antibodies from a naive combinatorial immunoglobulin library. , 1992, Proceedings of the National Academy of Sciences of the United States of America.

[9]  A. Pühler,et al.  Gene disruption and gene replacement in Streptomyces via single stranded DNA transformation of integration vectors. , 1991, Nucleic acids research.

[10]  J. Wells,et al.  Additivity of mutational effects in proteins. , 1990, Biochemistry.

[11]  J. Bailey,et al.  Expression of Recombinant Proteins in Escherichia coli Using an Oxygen-Responsive Promoter , 1990, Bio/Technology.

[12]  M. Eshoo,et al.  Selection, mapping, and characterization of osmoregulatory mutants of Escherichia coli blocked in the choline-glycine betaine pathway , 1986, Journal of bacteriology.

[13]  W. Rutter,et al.  Homology requirements for recombination in Escherichia coli. , 1985, Proceedings of the National Academy of Sciences of the United States of America.

[14]  Stanley N Cohen,et al.  Phenotypic expression in E. coli of a DNA sequence coding for mouse dihydrofolate reductase , 1978, Nature.

[15]  D. Goeddel,et al.  A method for random mutagenesis of a defined DNA segment using a modified polymerase chain reaction , 1989 .

[16]  A. Clark,et al.  6 – Pathways and Systems of Homologous Recombination in Escherichia coli , 1988 .

[17]  J. Vieira,et al.  Production of single-stranded plasmid DNA. , 1987, Methods in enzymology.

[18]  P. H. Son,et al.  Kilo-sequencing: creation of an ordered nest of asymmetric deletions across a large target sequence carried on phage M13. , 1983, Methods in enzymology.