Methionine-Mediated Regulation of Intestinal Lipid Transportation Induced by High-Fat Diet in Rice Field Eel (Monopterus Albus)

An eight-week feeding trial explored the mechanism that supplemented methionine (0 g/kg, 4 g/kg, 8 g/kg, and 12 g/kg) in a high-fat diet (120 g/kg fat) on intestinal lipid transportation and gut microbiota of M. Albus (initial weight 25.03 ± 0.13 g) based on the diet (60 g/kg fat), named as Con, HFD+M0, HFD+M4, HFD+M8, and HFD+M12, respectively. Compared with Con, gastric amylase, lipase, trypsin (P < 0.05), and intestinal lipase, amylase, trypsin, Na+/K+ -Adenosinetriphosphatase, depth of gastric fovea, and the number of intestinal villus goblet cells of HFD+M0 were markedly declined (P < 0.05), while intestinal high-density lipoprotein-cholesterol, very low-density lipoprotein-cholesterol and microsomal triglyceride transfer protein of HFD+M0 were markedly enhanced (P < 0.05); compared with HFD+M0, gastric lipase, amylase, trypsin, and intestinal lipase, trypsin, Na+/K+ -Adenosinetriphosphatase, microsomal triglyceride transfer protein, very low-density lipoprotein-cholesterol, and apolipoprotein -A, the height of intestinal villus and the number of intestinal villus goblet cells of HFD+M8 were remarkably enhanced (P < 0.05). Compared with Con, intestinal occ, cl12, cl15, zo-1, zo-2 of HFD + M0 were markedly down-regulated (P <0.05), while intestinal vldlr, npc1l1, cd36, fatp1, fatp2, fatp6, fatp7, apo, apoa, apob, apof, apoo, mct1, mct2, mct4, mct7, mct12, lpl, mttp, moat2, dgat2 of HFD M0 were remarkably upregulated (P < 0.05); compared with HFD+M0, intestinal gcn2 and eif2α of HFD+M8 were remarkably downregulated (P < 0.05), intestinal occ, cl12, cl15, zo-1, zo-2, hdlbp, ldlrap, vldlr, cd36, fatp1, fatp2, fatp6, apo, apoa, apob, apof, apoo, mct1, mct2, mct8, mct12, lpl, mttp, moat2, and dgat2 were remarkably upregulated (P < 0.05). Compared with Con, the diversity of gut microbiota of HFD+M0 was significantly declined (P < 0.05), while the diversity of gut microbiota in HFD+M8 was significantly higher than that in HFD+M0 (P < 0.05). In conclusion, a high-fat methionine deficiency diet destroyed the intestinal barrier, reduced the capacity of intestinal digestion and absorption, and disrupted the balance of gut microbiota; supplemented methionine promoted the digestion and absorption of lipids, and also improved the balance of gut microbiota.

[1]  W. Chu,et al.  A study on methionine-mediated regulation of muscle fiber growth, development and differentiation in the rice field eel (Monopterus albus) , 2022, Aquaculture.

[2]  K. Mai,et al.  Taurine supplements in high-fat diets improve survival of juvenile Monopterus albus by reducing lipid deposition and intestinal damage , 2022, Aquaculture.

[3]  R. Hardy,et al.  An initial evaluation of fishmeal replacement with soy protein sources on growth and immune responses of burbot (Lota lota maculosa) , 2021 .

[4]  Huan Zhong,et al.  A Study on How Methionine Restriction Decreases the Body’s Hepatic and Lipid Deposition in Rice Field Eel (Monopterus albus) , 2021, International Journal of Molecular Sciences.

[5]  Dongyu Huang,et al.  Dietary Clostridium autoethanogenum protein modulates intestinal absorption, antioxidant status, and immune response in GIFT ( Oreochromis niloticus ) juveniles , 2021, Aquaculture Research.

[6]  Xinli Li,et al.  Apple Polyphenol Extract Improves High-Fat Diet-Induced Hepatic Steatosis by Regulating Bile Acid Synthesis and Gut Microbiota in C57BL/6 Male Mice. , 2021, Journal of agricultural and food chemistry.

[7]  Y. Hu,et al.  Using unessential sulfur amino acids to overcome methionine deficient diets on rice field eel (Monopterus albus) , 2021 .

[8]  Yuanfa He,et al.  Changes in the PI3K/Akt/TOR signalling pathway after methionine treatment in the primary muscle cells of cobia ( rachycentron canadum ) , 2021 .

[9]  W. Younas,et al.  Dietary supplementations of methionine improve growth performances, innate immunity, digestive enzymes, and antioxidant activities of rohu (Labeo rohita) , 2021, Fish Physiology and Biochemistry.

[10]  Hua Tang,et al.  ApoPred: Identification of Apolipoproteins and Their Subfamilies With Multifarious Features , 2021, Frontiers in Cell and Developmental Biology.

[11]  Y. Hu,et al.  Effects of dietary soy isoflavone and soy saponin on growth performance, intestinal structure, intestinal immunity and gut microbiota community on rice field eel (Monopterus albus) , 2020, Aquaculture.

[12]  B. Han,et al.  Replacement of fishmeal with soybean meal affects the growth performance, digestive enzymes, intestinal microbiota and immunity of Carassius auratus gibelio♀ × Cyprinus carpio♂ , 2020 .

[13]  K. Mai,et al.  Total replacement of fish meal with soybean meal in diets for bullfrog (Lithobates catesbeianus): Effects on growth performance and gut microbial composition , 2020 .

[14]  Wenbing Zhang,et al.  Dietary taurine improves muscle growth and texture characteristics in juvenile turbot (Scophthalmus maximus) , 2020 .

[15]  F. Coppedè,et al.  Polymorphisms of genes required for methionine synthesis and DNA methylation influence mitochondrial DNA methylation. , 2020, Epigenomics.

[16]  Y. Hu,et al.  Effect of partial black soldier fly (Hermetia illucens L.) larvae meal replacement of fish meal in practical diets on the growth, digestive enzyme and related gene expression for rice field eel (Monopterus albus) , 2020 .

[17]  P. Domagała,et al.  Monocarboxylate Transporter 1 (MCT1) in Liver Pathology , 2020, International journal of molecular sciences.

[18]  K. Chiu,et al.  Decreased PEDF Promotes Hepatic Fatty Acid Uptake and Lipid Droplet Formation in the Pathogenesis of NAFLD , 2020, Nutrients.

[19]  Y. Hu,et al.  Effects of dietary vitamin C on growth, antioxidant activity, and immunity in ricefield eel ( Monopterus albus ) , 2020, Journal of the World Aquaculture Society.

[20]  Y. Taketani,et al.  High-fat diets provoke phosphorus absorption from the small intestine in rats. , 2019, Nutrition.

[21]  Tetsuya Nakamura,et al.  High-fat diet-derived free fatty acids impair the intestinal immune system and increase sensitivity to intestinal epithelial damage. , 2019, Biochemical and biophysical research communications.

[22]  R. Jannathulla,et al.  Fishmeal availability in the scenarios of climate change: Inevitability of fishmeal replacement in aquafeeds and approaches for the utilization of plant protein sources , 2019, Aquaculture Research.

[23]  Y. Hu,et al.  Replacement of fish meal with soy protein concentrate in diet of juvenile rice field eel Monopterus albus , 2019, Aquaculture Reports.

[24]  Y. Hu,et al.  Dysbiosis of intestinal microbiota induced by dietary oxidized fish oil and recovery of diet-induced dysbiosis via taurine supplementation in rice field eel (Monopterus albus) , 2019, Aquaculture.

[25]  Yuhui Yang,et al.  Dietary methionine restriction improves the gut microbiota and reduces intestinal permeability and inflammation in high-fat-fed mice. , 2019, Food & function.

[26]  W. Chu,et al.  Effects of High Dietary Levels of Cottonseed Meal and Rapeseed Meal on Growth Performance, Muscle Texture, and Expression of Muscle‐Related Genes in Grass Carp , 2019, North American Journal of Aquaculture.

[27]  S. Liao,et al.  Physiological Effects of Dietary Amino Acids on Gut Health and Functions of Swine , 2019, Front. Vet. Sci..

[28]  Junjing Xue,et al.  Disturbance in the homeostasis of intestinal microbiota by a high-fat diet in the rice field eel ( albus) , 2019, Aquaculture.

[29]  R. Finn,et al.  A new genomic blueprint of the human gut microbiota , 2019, Nature.

[30]  S. Snyder,et al.  Regulators of the transsulfuration pathway , 2018, British journal of pharmacology.

[31]  Zhi Luo,et al.  Upstream regulators of apoptosis mediates methionine-induced changes of lipid metabolism. , 2018, Cellular signalling.

[32]  Y. Duan,et al.  Optimal branched-chain amino acid ratio improves cell proliferation and protein metabolism of porcine enterocytesin in vivo and in vitro. , 2018, Nutrition.

[33]  Zhaolin Li,et al.  Effect of dietary taurine supplementation on growth, digestive enzyme, immunity and resistant to dry stress of rice field eel (Monopterus albus) fed low fish meal diets , 2018 .

[34]  Qicun Zhou,et al.  Effects of dietary soy protein concentrate meal on growth, immunity, enzyme activity and protein metabolism in relation to gene expression in large yellow croaker Larimichthys crocea , 2017 .

[35]  Ó. Monroig,et al.  Dietary DHA/EPA ratio affected tissue fatty acid profiles, antioxidant capacity, hematological characteristics and expression of lipid-related genes but not growth in juvenile black seabream (Acanthopagrus schlegelii) , 2017, PloS one.

[36]  X. Leng,et al.  Substitute of soy protein concentrate for fish meal in diets of white shrimp (Litopenaeus vannamei Boone) , 2017, Aquaculture International.

[37]  Ye Zhao,et al.  Effects of lysine and methionine supplementation on growth, body composition and digestive function of grass carp (Ctenopharyngodon idella) fed plant protein diets using high-level canola meal , 2016 .

[38]  Brian J. Bennett,et al.  Expanding role of gut microbiota in lipid metabolism , 2016, Current opinion in lipidology.

[39]  P. Puigserver,et al.  The Methionine Transamination Pathway Controls Hepatic Glucose Metabolism through Regulation of the GCN5 Acetyltransferase and the PGC-1α Transcriptional Coactivator* , 2016, The Journal of Biological Chemistry.

[40]  Kazumi Take,et al.  Pharmacological Inhibition of Monoacylglycerol O-Acyltransferase 2 Improves Hyperlipidemia, Obesity, and Diabetes by Change in Intestinal Fat Utilization , 2016, PloS one.

[41]  Wei Xu,et al.  Dietary methionine level influences growth and lipid metabolism via GCN2 pathway in cobia (Rachycentron canadum) , 2016 .

[42]  Yan Wang,et al.  Replacement of dietary fish meal by soybean meal supplemented with crystalline methionine for Japanese seabass (Lateolabrax japonicus). , 2016 .

[43]  P. Ferket,et al.  Effects of feed grade L-methionine on intestinal redox status, intestinal development, and growth performance of young chickens compared with conventional DL-methionine. , 2015, Journal of animal science.

[44]  B. van de Sluis,et al.  The life cycle of the low-density lipoprotein receptor: insights from cellular and in-vivo studies , 2015, Current opinion in lipidology.

[45]  D. Sabatini,et al.  Nutrient-sensing mechanisms and pathways , 2015, Nature.

[46]  S. W. Kim,et al.  Effect of feed grade L-methionine on growth performance and gut health in nursery pigs compared with conventional DL-methionine. , 2014, Journal of animal science.

[47]  T. Dinan,et al.  Minireview: Gut microbiota: the neglected endocrine organ. , 2014, Molecular endocrinology.

[48]  P. Besnard,et al.  From fatty-acid sensing to chylomicron synthesis: role of intestinal lipid-binding proteins. , 2014, Biochimie.

[49]  Q. Ai,et al.  Effects of practical dietary protein to lipid levels on growth, digestive enzyme activities and body composition of juvenile rice field eel (Monopterus albus) , 2014, Aquaculture International.

[50]  Chang-geng Yang,et al.  Changes in the activities and mRNA expression levels of lipoprotein lipase (LPL), hormone-sensitive lipase (HSL) and fatty acid synthetase (FAS) of Nile tilapia (Oreochromis niloticus) during fasting and re-feeding , 2013 .

[51]  P. M. Craig,et al.  Methionine restriction affects the phenotypic and transcriptional response of rainbow trout (Oncorhynchus mykiss) to carbohydrate-enriched diets. , 2013, The British journal of nutrition.

[52]  Rob Knight,et al.  Microbiota regulate intestinal absorption and metabolism of fatty acids in the zebrafish. , 2012, Cell host & microbe.

[53]  M. Hussain,et al.  Regulation of microsomal triglyceride transfer protein , 2011, Clinical lipidology.

[54]  P. Moughan,et al.  Regulation of tight junction permeability by intestinal bacteria and dietary components. , 2011, The Journal of nutrition.

[55]  S. Koonawootrittriron,et al.  Effects of adding liquid DL-methionine hydroxy analogue-free acid to drinking water on growth performance and small intestinal morphology of nursery pigs. , 2009, Journal of animal physiology and animal nutrition.

[56]  B. Stoll,et al.  Intestinal metabolism of sulfur amino acids , 2009, Nutrition Research Reviews.

[57]  S. Qiao,et al.  Amino acids and gut function , 2009, Amino Acids.

[58]  G. Shulman,et al.  Suppression of Diacylglycerol Acyltransferase-2 (DGAT2), but Not DGAT1, with Antisense Oligonucleotides Reverses Diet-induced Hepatic Steatosis and Insulin Resistance* , 2007, Journal of Biological Chemistry.

[59]  A. Lonardo,et al.  Hepatic steatosis and insulin resistance , 2005 .

[60]  Jen-Leih Wu,et al.  Molecular cloning and functional analysis of zebrafish high-density lipoprotein-binding protein. , 2003, Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology.

[61]  Thomas D. Schmittgen,et al.  Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. , 2001, Methods.

[62]  P. Kwiterovich.  The metabolic pathways of high-density lipoprotein, low-density lipoprotein, and triglycerides: a current review. , 2000, The American journal of cardiology.

[63]  G. Anantharamaiah,et al.  Structural models of human apolipoprotein A-I. , 1995, Biochimica et biophysica acta.

[64]  Thomas D. Schmittgen,et al.  Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2 2 DD C T Method , 2022 .