Comparative Evaluation of Different DNA Extraction Procedures from Food Samples

Five methodologies for extracting DNA from food samples are described. The food products analyzed are from either soybean or maize. They were selected on the basis of the mechanical, thermal, and chemical treatments that they had been subjected to during industrial processing. DNA preparations were evaluated for purity, yield, and average fragment size. Two endogenous genes, soybean lectin gene and alcohol dehydrogenase gene (adh1), were used to assess the degree of DNA degradation at different stages of the transformation chain. The goal of this study was to determine the role that extraction methods play in DNA amplification in order to select the best protocol for a food sample. This comparative evaluation can be specifically useful for detection of genetically modified ingredients in a variety of food matrices.

[1]  A. Zimmermann,et al.  Quantitative and qualitative evaluation of nine different extraction methods for nucleic acids on soya bean food samples , 1998 .

[2]  M. Sprenger-Haussels,et al.  PCR-based detection of genetically modified soybean and maize in raw and highly processed foodstuffs. , 2001, BioTechniques.

[3]  J. Cadet,et al.  Simple chromatographic systems permitting both DNA purification and separation of 2'-deoxyribonucleoside 3'-monophosphates as substrates for 32P-postlabelling studies. , 1993, Journal of chromatography.

[4]  J. Bernhagen,et al.  An agent cleaving glucose-derived protein crosslinks in vitro and in vivo , 1996, Nature.

[5]  Linus U. Opara,et al.  Traceability in agriculture and food supply chain: A review of basic concepts, technological implications, and future prospects , 2002 .

[6]  C. Hurst,et al.  PCR detection of genetically modified soya and maize in foodstuffs , 1999, Molecular breeding.

[7]  U. Galderisi,et al.  Methods To Improve the Yield and Quality of DNA from Dried and Processed Figs , 2008, Biotechnology progress.

[8]  W. F. Thompson,et al.  RAPID ISOLATION OF HIGH-MOLECULAR WEIGHT DNA , 1980 .

[9]  H. Hartings,et al.  Plant DNA extraction based on grinding by reciprocal shaking of dried tissue. , 2000, Analytical biochemistry.

[10]  Donna S. Smith,et al.  Comparison of several methods for the extraction of DNA from potatoes and potato-derived products. , 2005, Journal of agricultural and food chemistry.

[11]  S. Kanaya,et al.  Detection of tRNA Based on the Cloverleaf Secondary Structure , 2000 .

[12]  Kathy Messens,et al.  Evaluation and optimisation of five different extraction methods for soy DNA in chocolate and biscuits. Extraction of DNA as a first step in GMO analysis , 2004 .

[13]  Nelson Marmiroli,et al.  Qualitative and quantitative evaluation of the genomic DNA extracted from GMO and non-GMO foodstuffs with four different extraction methods. , 2004, Journal of agricultural and food chemistry.

[14]  THE AMERICAN ASSOCIATION OF CEREAL CHEMISTS. , 1942, Science.

[15]  Elke Anklam,et al.  Method development in relation to regulatory requirements for detection of GMOs in the food chain. , 2002, Journal of AOAC International.

[16]  S. Pääbo,et al.  Molecular coproscopy: dung and diet of the extinct ground sloth Nothrotheriops shastensis. , 1998, Science.